qPCR counts of mRNA for hepatic reference gene selection in adult female Atlantic salmon from perturbation experiments under artificial temperature conditions

The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for any...

Full description

Bibliographic Details
Main Authors: Anderson, Kelli, Elizur, Abigail
Format: Dataset
Language:English
Published: PANGAEA 2011
Subjects:
DATE/TIME
Event label
Exp_Tank_4m3
Experimental tank (4 cubic metre) under artificial conditions
Fluorescence-based quantitative real-time PCR (qPCR)
Life Cycle: state
qPCR counts
mean
ratio
standard error of mean
SALTAS_Experimental_Tank(14degC)_April_2008
SALTAS_Experimental_Tank(14degC)_August_2007
SALTAS_Experimental_Tank(14degC)_February_2008
SALTAS_Experimental_Tank(14degC)_January_2008
SALTAS_Experimental_Tank(14degC)_March_2008
SALTAS_Experimental_Tank(14degC)_November_2007
SALTAS_Experimental_Tank(22degC)_April_2008
SALTAS_Experimental_Tank(22degC)_February_2008
SALTAS_Experimental_Tank(22degC)_March_2008
Treatment: temperature
Atlantic salmon
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.772212
https://doi.org/10.1594/PANGAEA.772212
Description
Summary:The dataset contains raw data (quantification cycle) for a study which determined the most suitable hepatic reference genes for normalisation of qPCR data orginating from adult (entire reproductive season) Atlantic salmon (14 days) exposed to 14 and 22 degrees C. These results will be useful for anyone wanting to study the effects of climate change/elevated temperature on reproductive physiology of fish (and perhaphs other vertebrates). In addition, a target gene (vitellogenin) has normalised using an inappropriate and an 'ideal' reference gene to demonstrate the consequences of using an unstable reference gene for normalisation. For the adult experiment, maiden and repeat adult females were held at the Salmon Enterprises of Tasmania (SALTAS) Wayatinah Hatchery (Tasmania, Australia) at ambient temperature and photoperiod in either 200 (maidens) or 50 (repeats) m3 circular tanks at stocking densities of 12-18, and 24-36 kg m-3 for maidens and repeats, respectively, until transfered to the experimental tanks.

Similar Items