Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011
This study investigated the impact of photon flux and elevated CO2 concentrations on growth and photosynthetic electron transport on the marine diatom Chaetoceros muelleri and looked for evidence for the presence of a CO2-concentrating mechanism (CCM). pH drift experiments clearly showed that C. mue...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.769749 2024-09-15T18:28:13+00:00 Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 Ihnken, Sven Roberts, Simon Beardall, John 2011 text/tab-separated-values, 981 data points https://doi.pangaea.de/10.1594/PANGAEA.769749 https://doi.org/10.1594/PANGAEA.769749 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.769749 https://doi.org/10.1594/PANGAEA.769749 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Ihnken, Sven; Roberts, Simon; Beardall, John (2011): Differential responses of growth and photosynthesis in the marine diatom Chaetoceros muelleri to CO2 and light availability. Phycologia, 50(2), 182-193, https://doi.org/10.2216/10-11.1 Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros muelleri Chromista EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate standard deviation Identification Laboratory experiment Laboratory strains Light Light capturing capacity standard devitation Light saturation point Maximal electron transport rate relative OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH pH meter (Metrohm electrodes) Phytoplankton dataset 2011 ftpangaea https://doi.org/10.1594/PANGAEA.76974910.2216/10-11.1 2024-07-24T02:31:31Z This study investigated the impact of photon flux and elevated CO2 concentrations on growth and photosynthetic electron transport on the marine diatom Chaetoceros muelleri and looked for evidence for the presence of a CO2-concentrating mechanism (CCM). pH drift experiments clearly showed that C. muelleri has the capacity to use bicarbonate to acquire inorganic carbon through one or multiple CCMs. The final pH achieved in unbuffered cultures was not changed by light intensity, even under very low photon flux, implying a low energy demand of bicarbonate use via a CCM. In short-term pH drift experiments, only treatment with the carbonic anhydrase inhibitor ethoxyzolamide (EZ) slowed down the rise in pH considerably. EZ was also the only inhibitor that altered the final pH attained, although marginally. In growth experiments, CO2 availability was manipulated by changing the pH in closed flasks at a fixed dissolved inorganic carbon (DIC) concentration. Low-light-treated samples showed lower growth rates in elevated CO2conditions. No CO2 effect was recorded under high light exposure. The maximal photosynthetic capacity, however, increased with CO2 concentration in saturating, but not in subsaturating, light intensities. Growth and photosynthetic capacity therefore responded in opposite ways to increasing CO2 availability. The capacity to photoacclimate to high and low photon flux appeared not to be affected by CO2treatments. However, photoacclimation was restricted to growth photon fluxes between 30 and 300 µmol photons m-2 s-1. The light saturation points for photosynthetic electron transport and for growth coincided at 100 µmol photons m-2 s-1. Below 100 µmol photons m-2 s-1 the light saturation point for photosynthesis was higher than the growth photon flux (i.e. photosynthesis was not light saturated under growth conditions), whereas at higher growth photon flux, photosynthesis was saturated below growth light levels. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros muelleri Chromista EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate standard deviation Identification Laboratory experiment Laboratory strains Light Light capturing capacity standard devitation Light saturation point Maximal electron transport rate relative OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH pH meter (Metrohm electrodes) Phytoplankton |
spellingShingle |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros muelleri Chromista EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate standard deviation Identification Laboratory experiment Laboratory strains Light Light capturing capacity standard devitation Light saturation point Maximal electron transport rate relative OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH pH meter (Metrohm electrodes) Phytoplankton Ihnken, Sven Roberts, Simon Beardall, John Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 |
topic_facet |
Alkalinity total Aragonite saturation state Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chaetoceros muelleri Chromista EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate standard deviation Identification Laboratory experiment Laboratory strains Light Light capturing capacity standard devitation Light saturation point Maximal electron transport rate relative OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH pH meter (Metrohm electrodes) Phytoplankton |
description |
This study investigated the impact of photon flux and elevated CO2 concentrations on growth and photosynthetic electron transport on the marine diatom Chaetoceros muelleri and looked for evidence for the presence of a CO2-concentrating mechanism (CCM). pH drift experiments clearly showed that C. muelleri has the capacity to use bicarbonate to acquire inorganic carbon through one or multiple CCMs. The final pH achieved in unbuffered cultures was not changed by light intensity, even under very low photon flux, implying a low energy demand of bicarbonate use via a CCM. In short-term pH drift experiments, only treatment with the carbonic anhydrase inhibitor ethoxyzolamide (EZ) slowed down the rise in pH considerably. EZ was also the only inhibitor that altered the final pH attained, although marginally. In growth experiments, CO2 availability was manipulated by changing the pH in closed flasks at a fixed dissolved inorganic carbon (DIC) concentration. Low-light-treated samples showed lower growth rates in elevated CO2conditions. No CO2 effect was recorded under high light exposure. The maximal photosynthetic capacity, however, increased with CO2 concentration in saturating, but not in subsaturating, light intensities. Growth and photosynthetic capacity therefore responded in opposite ways to increasing CO2 availability. The capacity to photoacclimate to high and low photon flux appeared not to be affected by CO2treatments. However, photoacclimation was restricted to growth photon fluxes between 30 and 300 µmol photons m-2 s-1. The light saturation points for photosynthetic electron transport and for growth coincided at 100 µmol photons m-2 s-1. Below 100 µmol photons m-2 s-1 the light saturation point for photosynthesis was higher than the growth photon flux (i.e. photosynthesis was not light saturated under growth conditions), whereas at higher growth photon flux, photosynthesis was saturated below growth light levels. |
format |
Dataset |
author |
Ihnken, Sven Roberts, Simon Beardall, John |
author_facet |
Ihnken, Sven Roberts, Simon Beardall, John |
author_sort |
Ihnken, Sven |
title |
Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 |
title_short |
Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 |
title_full |
Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 |
title_fullStr |
Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 |
title_full_unstemmed |
Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 |
title_sort |
seawater carbonate chemistry, growth rate and light sensitivity of marine diatom chaetoceros muelleri (strain csiro cs-176) during experiments, 2011 |
publisher |
PANGAEA |
publishDate |
2011 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.769749 https://doi.org/10.1594/PANGAEA.769749 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Ihnken, Sven; Roberts, Simon; Beardall, John (2011): Differential responses of growth and photosynthesis in the marine diatom Chaetoceros muelleri to CO2 and light availability. Phycologia, 50(2), 182-193, https://doi.org/10.2216/10-11.1 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.769749 https://doi.org/10.1594/PANGAEA.769749 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.76974910.2216/10-11.1 |
_version_ |
1810469547093786624 |