Seawater carbonate chemistry and biological processes of Emiliania huxleyi (strains RCC1256 and NZEH) during experiments, 2011

• Main Authors: Hoppe, Clara Jule Marie, Langer, Gerald, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2011
• Subjects: Alkalinity; Gran titration (Gran; 1950); total; Aragonite saturation state; Bicarbonate ion; BIOACID; Biological Impacts of Ocean Acidification; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Calcite saturation state; Calculated; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; particulate; per cell; organic; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chromista; Comment; Emiliania huxleyi; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Haptophyta; Laboratory experiment; Laboratory strains; Mass spectrometer ANCA-SL 20-20 Europa Scientific; Mediterranean Sea Acidification in a Changing Climate; MedSeA; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate inorganic carbon/particulate organic carbon ratio; Rodriguez; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.763842; https://doi.org/10.1594/PANGAEA.763842

With respect to their sensitivity to ocean acidification, calcifiers such as the coccolithophore Emiliania huxleyi have received special attention, as the process of calcification seems to be particularly sensitive to changes in the marine carbonate system. For E. huxleyi, apparently conflicting results regarding its sensitivity to ocean acidification have been published (Iglesias-Rodriguez et al., 2008a; Riebesell et al., 2000). As possible causes for discrepancies, intra-specific variability and different effects of CO2 manipulation methods, i.e. the manipulation of total alkalinity (TA) or total dissolved inorganic carbon (DIC), have been discussed. While Langer et al. (2009) demonstrate a high degree of intra-specific variability between strains of E. huxleyi, the question whether different CO2 manipulation methods influence the cellular responses has not been resolved yet. In this study, closed TA as well as open and closed DIC manipulation methods were compared with respect to E. huxleyi's CO2-dependence in growth rate, POC- and PIC-production. The differences in the carbonate chemistry between TA and DIC manipulations were shown not to cause any differences in response patterns, while the latter differed between open and closed DIC manipulation. The two strains investigated showed different sensitivities to acidification of seawater, RCC1256 being more negatively affected in growth rates and PIC production than NZEH.