Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea
During the 'Polarstern' expedition ARK-IV/2 in June 1987, water samples from 8 stations were taken to study biomass and substrate utilization of cold adapted bacteria. Bacterial biomasses determined from acridine orange direct counts (AODC) were between 0.4 and 31.4 µ/g C/l, and ATP concen...
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1991
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.743935 2023-05-15T16:18:07+02:00 Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea Tan, Tjhing Lok Rüger, Hans-Jürgen MEDIAN LATITUDE: 75.512360 * MEDIAN LONGITUDE: -9.818920 * SOUTH-BOUND LATITUDE: 71.946660 * WEST-BOUND LONGITUDE: -17.520000 * NORTH-BOUND LATITUDE: 78.991670 * EAST-BOUND LONGITUDE: -0.205000 * DATE/TIME START: 1987-06-02T12:54:00 * DATE/TIME END: 1987-06-26T14:03:00 1991-08-05 application/zip, 2 datasets https://doi.pangaea.de/10.1594/PANGAEA.743935 https://doi.org/10.1594/PANGAEA.743935 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.743935 https://doi.org/10.1594/PANGAEA.743935 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Tan, Tjhing Lok; Rüger, Hans-Jürgen (1991): Biomass and nutritional requirements of psychrotrophic bacterial communities in Fram Strait and western Greenland Sea. Proceedings of the Fourth European Marine Micobiology Symposium, Kieler Meeresforschung, 8, 219-224, hdl:10013/epic.13649.d001 ARK-IV/2 CTD/Rosette CTD-RO North Greenland Sea Polarstern PS11 PS11/185-1 PS11/195-1 PS11/223-1 PS11/227-1 PS11/235-1 PS11/242-1 PS11/248-1 PS11/253-1 Dataset 1991 ftpangaea https://doi.org/10.1594/PANGAEA.743935 2023-01-20T07:31:52Z During the 'Polarstern' expedition ARK-IV/2 in June 1987, water samples from 8 stations were taken to study biomass and substrate utilization of cold adapted bacteria. Bacterial biomasses determined from acridine orange direct counts (AODC) were between 0.4 and 31.4 µ/g C/l, and ATP concentrations amounted from <0.1 to 40 ng/l. Colony counts on seawater agar reached only 0.1% of AODC, but with the MPN-method 1 to 10% of AODC were recorded. With 14C-glutamic acid or 14C-glucose as tracer substrate in oligotrophic broth containing 0.5 mg trypticase and 0.05 mg yeast extract per liter of seawater, obligately oligotrophic bacteria could be detected in one water sample. Although incubation was at 2 °C, only psychrotrophic bacteria showing growth temperatures between 1 and 30 °C were obtained. Organic substrate utilizations by 106 isolates were tested at 4 and 20 °C. Most carbohydrates, organic acids, alcohols, and alanine were assimilated at both temperatures, but arginine, aspartate and ornithine were utilized only at 20 °C by almost all strains. Dataset Fram Strait Greenland Greenland Sea North Greenland PANGAEA - Data Publisher for Earth & Environmental Science Greenland ENVELOPE(-17.520000,-0.205000,78.991670,71.946660) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
ARK-IV/2 CTD/Rosette CTD-RO North Greenland Sea Polarstern PS11 PS11/185-1 PS11/195-1 PS11/223-1 PS11/227-1 PS11/235-1 PS11/242-1 PS11/248-1 PS11/253-1 |
spellingShingle |
ARK-IV/2 CTD/Rosette CTD-RO North Greenland Sea Polarstern PS11 PS11/185-1 PS11/195-1 PS11/223-1 PS11/227-1 PS11/235-1 PS11/242-1 PS11/248-1 PS11/253-1 Tan, Tjhing Lok Rüger, Hans-Jürgen Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea |
topic_facet |
ARK-IV/2 CTD/Rosette CTD-RO North Greenland Sea Polarstern PS11 PS11/185-1 PS11/195-1 PS11/223-1 PS11/227-1 PS11/235-1 PS11/242-1 PS11/248-1 PS11/253-1 |
description |
During the 'Polarstern' expedition ARK-IV/2 in June 1987, water samples from 8 stations were taken to study biomass and substrate utilization of cold adapted bacteria. Bacterial biomasses determined from acridine orange direct counts (AODC) were between 0.4 and 31.4 µ/g C/l, and ATP concentrations amounted from <0.1 to 40 ng/l. Colony counts on seawater agar reached only 0.1% of AODC, but with the MPN-method 1 to 10% of AODC were recorded. With 14C-glutamic acid or 14C-glucose as tracer substrate in oligotrophic broth containing 0.5 mg trypticase and 0.05 mg yeast extract per liter of seawater, obligately oligotrophic bacteria could be detected in one water sample. Although incubation was at 2 °C, only psychrotrophic bacteria showing growth temperatures between 1 and 30 °C were obtained. Organic substrate utilizations by 106 isolates were tested at 4 and 20 °C. Most carbohydrates, organic acids, alcohols, and alanine were assimilated at both temperatures, but arginine, aspartate and ornithine were utilized only at 20 °C by almost all strains. |
format |
Dataset |
author |
Tan, Tjhing Lok Rüger, Hans-Jürgen |
author_facet |
Tan, Tjhing Lok Rüger, Hans-Jürgen |
author_sort |
Tan, Tjhing Lok |
title |
Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea |
title_short |
Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea |
title_full |
Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea |
title_fullStr |
Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea |
title_full_unstemmed |
Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea |
title_sort |
biomass and substrate utilization of cold adapted bacteria in fram strait and the western greenlad sea |
publisher |
PANGAEA |
publishDate |
1991 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.743935 https://doi.org/10.1594/PANGAEA.743935 |
op_coverage |
MEDIAN LATITUDE: 75.512360 * MEDIAN LONGITUDE: -9.818920 * SOUTH-BOUND LATITUDE: 71.946660 * WEST-BOUND LONGITUDE: -17.520000 * NORTH-BOUND LATITUDE: 78.991670 * EAST-BOUND LONGITUDE: -0.205000 * DATE/TIME START: 1987-06-02T12:54:00 * DATE/TIME END: 1987-06-26T14:03:00 |
long_lat |
ENVELOPE(-17.520000,-0.205000,78.991670,71.946660) |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Fram Strait Greenland Greenland Sea North Greenland |
genre_facet |
Fram Strait Greenland Greenland Sea North Greenland |
op_source |
Supplement to: Tan, Tjhing Lok; Rüger, Hans-Jürgen (1991): Biomass and nutritional requirements of psychrotrophic bacterial communities in Fram Strait and western Greenland Sea. Proceedings of the Fourth European Marine Micobiology Symposium, Kieler Meeresforschung, 8, 219-224, hdl:10013/epic.13649.d001 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.743935 https://doi.org/10.1594/PANGAEA.743935 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.743935 |
_version_ |
1766004250085687296 |