Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000
We have measured the stable carbon isotopic composition of bulk organic matter (POC), alkenones, sterols, fatty acids, and phytol in the coccolithophorid Emiliania huxleyi grown in dilute batch cultures over a wide range of CO2 concentrations (1.1-53.5 micromol L-1). The carbon isotope fractionation...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.727288 2024-09-15T18:24:30+00:00 Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 Riebesell, Ulf Revill, Andrew T Holdsworth, Daniel G Volkman, J K 2000 text/tab-separated-values, 286 data points https://doi.pangaea.de/10.1594/PANGAEA.727288 https://doi.org/10.1594/PANGAEA.727288 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.727288 https://doi.org/10.1594/PANGAEA.727288 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Riebesell, Ulf; Revill, Andrew T; Holdsworth, Daniel G; Volkman, J K (2000): The effects of varying CO2 concentration on lipid composition and carbon isotope fractionation in Emiliania huxleyi. Geochimica et Cosmochimica Acta, 64(24), 4179-4192, https://doi.org/10.1016/S0016-7037(00)00474-9 Alkalinity Gran titration (Gran 1950) total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated see reference(s) Calculated after Freeman & Hayes (1992) Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbon per cell Chromista Coulometric titration CTD Sea-Bird SBE 911plus Emiliania huxleyi EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification EXP Experiment Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haptophyta Isotopic fractionation during photosynthis Laboratory experiment Laboratory strains Mass spectrometer ANCA-SL 20-20 Europa Scientific Mass spectrometer Finnigan Delta-S Nitrogen/Carbon ratio Nitrogen per cell North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process dataset 2000 ftpangaea https://doi.org/10.1594/PANGAEA.72728810.1016/S0016-7037(00)00474-9 2024-07-24T02:31:30Z We have measured the stable carbon isotopic composition of bulk organic matter (POC), alkenones, sterols, fatty acids, and phytol in the coccolithophorid Emiliania huxleyi grown in dilute batch cultures over a wide range of CO2 concentrations (1.1-53.5 micromol L-1). The carbon isotope fractionation of POC (POC) varied by ca. 7 per mil and was positively correlated with aqueous CO2 concentration [CO2aq]. While this result confirms general trends observed for the same alga grown in nitrogen-limited chemostat cultures, considerable differences were obtained in absolute values of POC and in the slope of the relationship of POC with growth rate and [CO2aq]. Also, a significantly greater offset was obtained between the delta13C of alkenones and bulk organic matter in this study compared with previous work (5.4, cf. 3.8 per mil). This suggests that the magnitude of the isotope offset may depend on growth conditions. Relative to POC, individual fatty acids were depleted in 13C by 2.3 per mil to 4.1 per mil, phytol was depleted in 13C by 1.9 per mil, and the major sterol 24-methylcholesta-5,22E-dien-3beta-ol was depleted in 13C by 8.5 per mil. This large spread of delta13C values for different lipid classes in the same alga indicates the need for caution in organic geochemical studies when assigning different sources to lipids that might have delta13C values differing by just a few per mil. Increases in [CO2aq] led to dramatic increases in the alkenone contents per cell and as a proportion of organic carbon, but there was no systematic effect on values of U37k- used for reconstructions of paleo sea surface temperature. Dataset North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
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Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity Gran titration (Gran 1950) total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated see reference(s) Calculated after Freeman & Hayes (1992) Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbon per cell Chromista Coulometric titration CTD Sea-Bird SBE 911plus Emiliania huxleyi EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification EXP Experiment Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haptophyta Isotopic fractionation during photosynthis Laboratory experiment Laboratory strains Mass spectrometer ANCA-SL 20-20 Europa Scientific Mass spectrometer Finnigan Delta-S Nitrogen/Carbon ratio Nitrogen per cell North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process |
spellingShingle |
Alkalinity Gran titration (Gran 1950) total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated see reference(s) Calculated after Freeman & Hayes (1992) Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbon per cell Chromista Coulometric titration CTD Sea-Bird SBE 911plus Emiliania huxleyi EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification EXP Experiment Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haptophyta Isotopic fractionation during photosynthis Laboratory experiment Laboratory strains Mass spectrometer ANCA-SL 20-20 Europa Scientific Mass spectrometer Finnigan Delta-S Nitrogen/Carbon ratio Nitrogen per cell North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Riebesell, Ulf Revill, Andrew T Holdsworth, Daniel G Volkman, J K Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 |
topic_facet |
Alkalinity Gran titration (Gran 1950) total Aragonite saturation state Bicarbonate ion Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated see reference(s) Calculated after Freeman & Hayes (1992) Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Carbon per cell Chromista Coulometric titration CTD Sea-Bird SBE 911plus Emiliania huxleyi EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification EXP Experiment Experimental treatment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Growth/Morphology Growth rate Haptophyta Isotopic fractionation during photosynthis Laboratory experiment Laboratory strains Mass spectrometer ANCA-SL 20-20 Europa Scientific Mass spectrometer Finnigan Delta-S Nitrogen/Carbon ratio Nitrogen per cell North Atlantic OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process |
description |
We have measured the stable carbon isotopic composition of bulk organic matter (POC), alkenones, sterols, fatty acids, and phytol in the coccolithophorid Emiliania huxleyi grown in dilute batch cultures over a wide range of CO2 concentrations (1.1-53.5 micromol L-1). The carbon isotope fractionation of POC (POC) varied by ca. 7 per mil and was positively correlated with aqueous CO2 concentration [CO2aq]. While this result confirms general trends observed for the same alga grown in nitrogen-limited chemostat cultures, considerable differences were obtained in absolute values of POC and in the slope of the relationship of POC with growth rate and [CO2aq]. Also, a significantly greater offset was obtained between the delta13C of alkenones and bulk organic matter in this study compared with previous work (5.4, cf. 3.8 per mil). This suggests that the magnitude of the isotope offset may depend on growth conditions. Relative to POC, individual fatty acids were depleted in 13C by 2.3 per mil to 4.1 per mil, phytol was depleted in 13C by 1.9 per mil, and the major sterol 24-methylcholesta-5,22E-dien-3beta-ol was depleted in 13C by 8.5 per mil. This large spread of delta13C values for different lipid classes in the same alga indicates the need for caution in organic geochemical studies when assigning different sources to lipids that might have delta13C values differing by just a few per mil. Increases in [CO2aq] led to dramatic increases in the alkenone contents per cell and as a proportion of organic carbon, but there was no systematic effect on values of U37k- used for reconstructions of paleo sea surface temperature. |
format |
Dataset |
author |
Riebesell, Ulf Revill, Andrew T Holdsworth, Daniel G Volkman, J K |
author_facet |
Riebesell, Ulf Revill, Andrew T Holdsworth, Daniel G Volkman, J K |
author_sort |
Riebesell, Ulf |
title |
Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 |
title_short |
Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 |
title_full |
Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 |
title_fullStr |
Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 |
title_full_unstemmed |
Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 |
title_sort |
seawater carbonate chemistry and processes during experiments with emiliania huxleyi (pml b92/11), 2000 |
publisher |
PANGAEA |
publishDate |
2000 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.727288 https://doi.org/10.1594/PANGAEA.727288 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_source |
Supplement to: Riebesell, Ulf; Revill, Andrew T; Holdsworth, Daniel G; Volkman, J K (2000): The effects of varying CO2 concentration on lipid composition and carbon isotope fractionation in Emiliania huxleyi. Geochimica et Cosmochimica Acta, 64(24), 4179-4192, https://doi.org/10.1016/S0016-7037(00)00474-9 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.727288 https://doi.org/10.1594/PANGAEA.727288 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.72728810.1016/S0016-7037(00)00474-9 |
_version_ |
1810464871490256896 |