Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004
Opal accumulation rates in sediments have been used as a proxy for carbon flux, but there is poor understanding of the factors that regulate the Si quota of diatoms. Natural variation in silicon isotopes (delta.lc.gif - 54 Bytes30Si) in diatom frustules recovered from sediment cores are an alternati...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.721775 2024-09-15T18:28:11+00:00 Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004 Milligan, Allen J Varela, Diana E Brzezinski, Mark A Morel, Francois M M 2004 text/tab-separated-values, 371 data points https://doi.pangaea.de/10.1594/PANGAEA.721775 https://doi.org/10.1594/PANGAEA.721775 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.721775 https://doi.org/10.1594/PANGAEA.721775 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Milligan, Allen J; Varela, Diana E; Brzezinski, Mark A; Morel, Francois M M (2004): Dynamics of silicon metabolism and silicon isotopic discrimination in a marine diatom as a function of pCO2. Limnology and Oceanography, 49(2), 322-329, https://doi.org/10.4319/lo.2004.49.2.0322 Alkalinity total Aragonite saturation state Bicarbonate ion Biogenic silicate quota in diatom Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Colorimetry EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Event label EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Measured Milligan_etal_04/F2A Milligan_etal_04/F2B Milligan_etal_04/F5A Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton Radiation photosynthetically active Salinity Silicate efflux in diatom Silicate quota in diatom Single species Temperature water Thalassiosira weissflogii Time in minutes dataset 2004 ftpangaea https://doi.org/10.1594/PANGAEA.72177510.4319/lo.2004.49.2.0322 2024-07-24T02:31:30Z Opal accumulation rates in sediments have been used as a proxy for carbon flux, but there is poor understanding of the factors that regulate the Si quota of diatoms. Natural variation in silicon isotopes (delta.lc.gif - 54 Bytes30Si) in diatom frustules recovered from sediment cores are an alternative to opal mass for reconstructing diatom Si use and potential C export over geological timescales. Understanding the physiological factors that may influence the Si quota and the delta.lc.gif - 54 Bytes30Si isotopic signal is vital for interpreting biogenic silica as a paleoproxy. We investigated the influence of pCO2 on the Si quota, fluxes across the cell membrane, and frustule dissolution in the marine diatom Thalassiosira weissflogii and determined the effect that pCO2 has on the isotopic fractionation of Si. We found that our Si flux estimates mass balance and, for the first time, describe the Si budget of a diatom. The Si quota rose in cells grown with low pCO2 (100 ppm) compared with controls (370 ppm), and the increased quota was the result of greater retention of Si (i.e., lower losses of Si through efflux and dissolution). The ratio of efflux : influx decreased twofold as pCO2 decreased from 750 to 100 ppm. The efflux of silicon is shown to significantly bias measurements of silica dissolution rates determined by isotope dilution, but no effect on the Si isotopic enrichment factor (epsilon.lc.gif - 51 Bytes) was observed. The latter effect suggests that silicon isotopic discrimination in diatoms is set by the Si transport step rather than by the polymerization step. This observation supports the use of the v signal of biogenic silica as an indicator of the percentage utilization of silicic acid. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Aragonite saturation state Bicarbonate ion Biogenic silicate quota in diatom Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Colorimetry EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Event label EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Measured Milligan_etal_04/F2A Milligan_etal_04/F2B Milligan_etal_04/F5A Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton Radiation photosynthetically active Salinity Silicate efflux in diatom Silicate quota in diatom Single species Temperature water Thalassiosira weissflogii Time in minutes |
spellingShingle |
Alkalinity total Aragonite saturation state Bicarbonate ion Biogenic silicate quota in diatom Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Colorimetry EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Event label EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Measured Milligan_etal_04/F2A Milligan_etal_04/F2B Milligan_etal_04/F5A Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton Radiation photosynthetically active Salinity Silicate efflux in diatom Silicate quota in diatom Single species Temperature water Thalassiosira weissflogii Time in minutes Milligan, Allen J Varela, Diana E Brzezinski, Mark A Morel, Francois M M Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004 |
topic_facet |
Alkalinity total Aragonite saturation state Bicarbonate ion Biogenic silicate quota in diatom Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chromista Colorimetry EPOCA EUR-OCEANS European network of excellence for Ocean Ecosystems Analysis European Project on Ocean Acidification Event label EXP Experiment Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Laboratory strains Measured Milligan_etal_04/F2A Milligan_etal_04/F2B Milligan_etal_04/F5A Not applicable OA-ICC Ocean Acidification International Coordination Centre Ochrophyta Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pelagos pH Phytoplankton Radiation photosynthetically active Salinity Silicate efflux in diatom Silicate quota in diatom Single species Temperature water Thalassiosira weissflogii Time in minutes |
description |
Opal accumulation rates in sediments have been used as a proxy for carbon flux, but there is poor understanding of the factors that regulate the Si quota of diatoms. Natural variation in silicon isotopes (delta.lc.gif - 54 Bytes30Si) in diatom frustules recovered from sediment cores are an alternative to opal mass for reconstructing diatom Si use and potential C export over geological timescales. Understanding the physiological factors that may influence the Si quota and the delta.lc.gif - 54 Bytes30Si isotopic signal is vital for interpreting biogenic silica as a paleoproxy. We investigated the influence of pCO2 on the Si quota, fluxes across the cell membrane, and frustule dissolution in the marine diatom Thalassiosira weissflogii and determined the effect that pCO2 has on the isotopic fractionation of Si. We found that our Si flux estimates mass balance and, for the first time, describe the Si budget of a diatom. The Si quota rose in cells grown with low pCO2 (100 ppm) compared with controls (370 ppm), and the increased quota was the result of greater retention of Si (i.e., lower losses of Si through efflux and dissolution). The ratio of efflux : influx decreased twofold as pCO2 decreased from 750 to 100 ppm. The efflux of silicon is shown to significantly bias measurements of silica dissolution rates determined by isotope dilution, but no effect on the Si isotopic enrichment factor (epsilon.lc.gif - 51 Bytes) was observed. The latter effect suggests that silicon isotopic discrimination in diatoms is set by the Si transport step rather than by the polymerization step. This observation supports the use of the v signal of biogenic silica as an indicator of the percentage utilization of silicic acid. |
format |
Dataset |
author |
Milligan, Allen J Varela, Diana E Brzezinski, Mark A Morel, Francois M M |
author_facet |
Milligan, Allen J Varela, Diana E Brzezinski, Mark A Morel, Francois M M |
author_sort |
Milligan, Allen J |
title |
Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004 |
title_short |
Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004 |
title_full |
Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004 |
title_fullStr |
Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004 |
title_full_unstemmed |
Seawater carbonate chemistry and silica during an experiment with a marine diatom Thalassiosira weissflogii, 2004 |
title_sort |
seawater carbonate chemistry and silica during an experiment with a marine diatom thalassiosira weissflogii, 2004 |
publisher |
PANGAEA |
publishDate |
2004 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.721775 https://doi.org/10.1594/PANGAEA.721775 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Milligan, Allen J; Varela, Diana E; Brzezinski, Mark A; Morel, Francois M M (2004): Dynamics of silicon metabolism and silicon isotopic discrimination in a marine diatom as a function of pCO2. Limnology and Oceanography, 49(2), 322-329, https://doi.org/10.4319/lo.2004.49.2.0322 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.721775 https://doi.org/10.1594/PANGAEA.721775 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.72177510.4319/lo.2004.49.2.0322 |
_version_ |
1810469505732706304 |