Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean
Melatonin, the chief secretory product of the vertebrate pineal gland is suspected to be a ubiquitous molecule principally involved in the transduction of photoperiodic information. Besides vertebrates, melatonin has been detected throughout phylogeny in numerous non-vertebrate taxa. In the present...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.667467 2023-05-15T13:42:09+02:00 Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean Pape, Carsten Teschke, Mathias Meyer, Bettina MEDIAN LATITUDE: -65.154100 * MEDIAN LONGITUDE: 0.236875 * SOUTH-BOUND LATITUDE: -69.500600 * WEST-BOUND LONGITUDE: -3.027200 * NORTH-BOUND LATITUDE: -56.014800 * EAST-BOUND LONGITUDE: 3.368700 * DATE/TIME START: 2005-12-07T03:44:00 * DATE/TIME END: 2006-08-14T18:30:00 2007-11-08 application/zip, 14 datasets https://doi.pangaea.de/10.1594/PANGAEA.667467 https://doi.org/10.1594/PANGAEA.667467 en eng PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.667467 https://doi.org/10.1594/PANGAEA.667467 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess CC-BY Supplement to: Pape, Carsten; Teschke, Mathias; Meyer, Bettina (2008): Melatonin and its possible role in mediating seasonal metabolic changes of Antarctic krill, Euphausia superba. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 149(4), 426-434, https://doi.org/10.1016/j.cbpa.2008.02.001 ANT-XXIII/2 ANT-XXIII/6 AWI_BioOce Biological Oceanography @ AWI Polarstern PS69 PS69/043-2 PS69/046-1 PS69/078-1 PS69/092-1 PS69/474-1 PS69/489-1 PS69/497-1 PS69/506-7 PS69/518-1 PS69/520-1 PS69/534-1 Rectangular midwater trawl RMT Dataset 2007 ftpangaea https://doi.org/10.1594/PANGAEA.667467 https://doi.org/10.1016/j.cbpa.2008.02.001 2023-01-20T07:31:05Z Melatonin, the chief secretory product of the vertebrate pineal gland is suspected to be a ubiquitous molecule principally involved in the transduction of photoperiodic information. Besides vertebrates, melatonin has been detected throughout phylogeny in numerous non-vertebrate taxa. In the present study, the occurrence of melatonin in Antarctic krill Euphausia superba and its possible role in mediating seasonal metabolic changes was evaluated. Melatonin was quantified by enzyme linked immunosorbent assay (ELISA) in high performance liquid chromatography (HPLC) purified extracts of eyestalks and hemolymph of krill sampled in the Lazarev Sea during the Antarctic winter and summer. In addition, oxygen uptake rates and the activities of the metabolic enzyme malate dehydrogenase (MDH) were recorded to assess the metabolic status of krill. Validation of melatonin measurements was carried out on the basis of three different extraction methods with parallel determination of melatonin by ELISA in crude extracts and in HPLC purified extracts, and after derivatization of melatonin under alkaline conditions in the presence of hydrogen peroxide. A significantly higher respiration rate and MDH activity was found in summer krill than in winter krill indicating that krill was in a state of reduced metabolic activity during winter. However, neither during winter nor during summer there were detectable melatonin concentrations in the visual system or hemolymph of krill. Based on these results, we question a mediating role of melatonin in the control of seasonal metabolic changes in Antarctic krill in particular and its physiological significance in krill in general. Dataset Antarc* Antarctic Antarctic Krill Euphausia superba Lazarev Sea South Atlantic Ocean PANGAEA - Data Publisher for Earth & Environmental Science Antarctic Lazarev ENVELOPE(12.917,12.917,-69.967,-69.967) Lazarev Sea ENVELOPE(7.000,7.000,-68.000,-68.000) The Antarctic ENVELOPE(-3.027200,3.368700,-56.014800,-69.500600) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
ANT-XXIII/2 ANT-XXIII/6 AWI_BioOce Biological Oceanography @ AWI Polarstern PS69 PS69/043-2 PS69/046-1 PS69/078-1 PS69/092-1 PS69/474-1 PS69/489-1 PS69/497-1 PS69/506-7 PS69/518-1 PS69/520-1 PS69/534-1 Rectangular midwater trawl RMT |
spellingShingle |
ANT-XXIII/2 ANT-XXIII/6 AWI_BioOce Biological Oceanography @ AWI Polarstern PS69 PS69/043-2 PS69/046-1 PS69/078-1 PS69/092-1 PS69/474-1 PS69/489-1 PS69/497-1 PS69/506-7 PS69/518-1 PS69/520-1 PS69/534-1 Rectangular midwater trawl RMT Pape, Carsten Teschke, Mathias Meyer, Bettina Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean |
topic_facet |
ANT-XXIII/2 ANT-XXIII/6 AWI_BioOce Biological Oceanography @ AWI Polarstern PS69 PS69/043-2 PS69/046-1 PS69/078-1 PS69/092-1 PS69/474-1 PS69/489-1 PS69/497-1 PS69/506-7 PS69/518-1 PS69/520-1 PS69/534-1 Rectangular midwater trawl RMT |
description |
Melatonin, the chief secretory product of the vertebrate pineal gland is suspected to be a ubiquitous molecule principally involved in the transduction of photoperiodic information. Besides vertebrates, melatonin has been detected throughout phylogeny in numerous non-vertebrate taxa. In the present study, the occurrence of melatonin in Antarctic krill Euphausia superba and its possible role in mediating seasonal metabolic changes was evaluated. Melatonin was quantified by enzyme linked immunosorbent assay (ELISA) in high performance liquid chromatography (HPLC) purified extracts of eyestalks and hemolymph of krill sampled in the Lazarev Sea during the Antarctic winter and summer. In addition, oxygen uptake rates and the activities of the metabolic enzyme malate dehydrogenase (MDH) were recorded to assess the metabolic status of krill. Validation of melatonin measurements was carried out on the basis of three different extraction methods with parallel determination of melatonin by ELISA in crude extracts and in HPLC purified extracts, and after derivatization of melatonin under alkaline conditions in the presence of hydrogen peroxide. A significantly higher respiration rate and MDH activity was found in summer krill than in winter krill indicating that krill was in a state of reduced metabolic activity during winter. However, neither during winter nor during summer there were detectable melatonin concentrations in the visual system or hemolymph of krill. Based on these results, we question a mediating role of melatonin in the control of seasonal metabolic changes in Antarctic krill in particular and its physiological significance in krill in general. |
format |
Dataset |
author |
Pape, Carsten Teschke, Mathias Meyer, Bettina |
author_facet |
Pape, Carsten Teschke, Mathias Meyer, Bettina |
author_sort |
Pape, Carsten |
title |
Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean |
title_short |
Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean |
title_full |
Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean |
title_fullStr |
Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean |
title_full_unstemmed |
Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean |
title_sort |
oxygen respiration and metabolic enzyme malate dehydrogenase of euphasia superba in the south atlantic ocean |
publisher |
PANGAEA |
publishDate |
2007 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.667467 https://doi.org/10.1594/PANGAEA.667467 |
op_coverage |
MEDIAN LATITUDE: -65.154100 * MEDIAN LONGITUDE: 0.236875 * SOUTH-BOUND LATITUDE: -69.500600 * WEST-BOUND LONGITUDE: -3.027200 * NORTH-BOUND LATITUDE: -56.014800 * EAST-BOUND LONGITUDE: 3.368700 * DATE/TIME START: 2005-12-07T03:44:00 * DATE/TIME END: 2006-08-14T18:30:00 |
long_lat |
ENVELOPE(12.917,12.917,-69.967,-69.967) ENVELOPE(7.000,7.000,-68.000,-68.000) ENVELOPE(-3.027200,3.368700,-56.014800,-69.500600) |
geographic |
Antarctic Lazarev Lazarev Sea The Antarctic |
geographic_facet |
Antarctic Lazarev Lazarev Sea The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Lazarev Sea South Atlantic Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Lazarev Sea South Atlantic Ocean |
op_source |
Supplement to: Pape, Carsten; Teschke, Mathias; Meyer, Bettina (2008): Melatonin and its possible role in mediating seasonal metabolic changes of Antarctic krill, Euphausia superba. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 149(4), 426-434, https://doi.org/10.1016/j.cbpa.2008.02.001 |
op_relation |
https://doi.pangaea.de/10.1594/PANGAEA.667467 https://doi.org/10.1594/PANGAEA.667467 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/PANGAEA.667467 https://doi.org/10.1016/j.cbpa.2008.02.001 |
_version_ |
1766163252827389952 |