Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year
Introduction: Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. Methodology: The s...
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ftpamukkaleuniv:oai:acikerisim.pau.edu.tr:11499/32156 2023-05-15T16:04:56+02:00 Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE Caliskan, A Cicek, AC Ejder, NA Karagoz, A Kirisci, O Kilic, S 2019 http://hdl.handle.net/11499/32156 English eng J INFECTION DEVELOPING COUNTRIES 10.3855/jidc.11171 JOURNAL OF INFECTION IN DEVELOPING COUNTRIES 1972-2680 J9 J INFECT DEV COUNTR http://hdl.handle.net/11499/32156 13 7 634 639 WOS Pulsed Field Gel Electrophoresis clonal outbreak antibiotic sensitivity Article 2019 ftpamukkaleuniv 2020-11-08T10:04:40Z Introduction: Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. Methodology: The study included 153 S. maltophilia strains isolated from clinical samples sent to our hospital laboratory between 1 January 2014 and 30 June 2018. The bacteria were identified and their antibiotic sensitivity was determined using the VITEK-2 automated system. PFGE (Pulsed Field Gel Electrophoresis): The strains isolated from 34 patient clinical samples and from 1 patient bedcover were taken for PFGE examination. Results: The TMP/SXT and levofloxacin sensitivity of 153 S. maltophilia strains was examined. TMP/SXT resistance was determined to be 39% and levofloxacin resistance at 5%. Among 35 S. maltophilia strains, seven genotypes were identified using the PFGE method. While three strains showed a specific genotype profile, the other 32 were determined to consist of four clusters. The cluster rate was therefore 91.4% (32/35). Conclusions: There was a clonal relationship between the vast majority of the 35 S. maltophilia isolates, which suggests that there was a cross-contamination problem in the hospital. One strain (#4) was identified by dendrogram analysis showed a high rate of similarity to the other strains and was determined to be the common source of the cross-contamination. C1 [Caliskan, Ahmet] Pamukkale Univ, Fac Med, Dept Med Microbiol, Denizli, Turkey. [Cicek, Aysegul Copur; Ejder, Nebahat Aydogan] Recep Tayyip Erdogan Univ, Fac Med, Dept Med Microbiol, Rize, Turkey. [Karagoz, Alper] Usak Univ, Dept Microbiol Mol Biol & Genet, Usak, Turkey. [Kirisci, Ozlem] Necip Fazil City Hosp, Microbiol Lab, Kahramanmaras, Turkey. [Kilic, Selcuk] Publ Hlth Inst Turkey, Microbiol Reference Labs Dept, Ankara, Turkey. Article in Journal/Newspaper ejder Pamukkale University Repository |
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Pamukkale University Repository |
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English |
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Pulsed Field Gel Electrophoresis clonal outbreak antibiotic sensitivity |
spellingShingle |
Pulsed Field Gel Electrophoresis clonal outbreak antibiotic sensitivity Caliskan, A Cicek, AC Ejder, NA Karagoz, A Kirisci, O Kilic, S Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year |
topic_facet |
Pulsed Field Gel Electrophoresis clonal outbreak antibiotic sensitivity |
description |
Introduction: Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. Methodology: The study included 153 S. maltophilia strains isolated from clinical samples sent to our hospital laboratory between 1 January 2014 and 30 June 2018. The bacteria were identified and their antibiotic sensitivity was determined using the VITEK-2 automated system. PFGE (Pulsed Field Gel Electrophoresis): The strains isolated from 34 patient clinical samples and from 1 patient bedcover were taken for PFGE examination. Results: The TMP/SXT and levofloxacin sensitivity of 153 S. maltophilia strains was examined. TMP/SXT resistance was determined to be 39% and levofloxacin resistance at 5%. Among 35 S. maltophilia strains, seven genotypes were identified using the PFGE method. While three strains showed a specific genotype profile, the other 32 were determined to consist of four clusters. The cluster rate was therefore 91.4% (32/35). Conclusions: There was a clonal relationship between the vast majority of the 35 S. maltophilia isolates, which suggests that there was a cross-contamination problem in the hospital. One strain (#4) was identified by dendrogram analysis showed a high rate of similarity to the other strains and was determined to be the common source of the cross-contamination. C1 [Caliskan, Ahmet] Pamukkale Univ, Fac Med, Dept Med Microbiol, Denizli, Turkey. [Cicek, Aysegul Copur; Ejder, Nebahat Aydogan] Recep Tayyip Erdogan Univ, Fac Med, Dept Med Microbiol, Rize, Turkey. [Karagoz, Alper] Usak Univ, Dept Microbiol Mol Biol & Genet, Usak, Turkey. [Kirisci, Ozlem] Necip Fazil City Hosp, Microbiol Lab, Kahramanmaras, Turkey. [Kilic, Selcuk] Publ Hlth Inst Turkey, Microbiol Reference Labs Dept, Ankara, Turkey. |
format |
Article in Journal/Newspaper |
author |
Caliskan, A Cicek, AC Ejder, NA Karagoz, A Kirisci, O Kilic, S |
author_facet |
Caliskan, A Cicek, AC Ejder, NA Karagoz, A Kirisci, O Kilic, S |
author_sort |
Caliskan, A |
title |
Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year |
title_short |
Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year |
title_full |
Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year |
title_fullStr |
Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year |
title_full_unstemmed |
Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year |
title_sort |
antibiotic sensitivity of stenotrophomonas maltophilia in a 5-year |
publisher |
J INFECTION DEVELOPING COUNTRIES |
publishDate |
2019 |
url |
http://hdl.handle.net/11499/32156 |
genre |
ejder |
genre_facet |
ejder |
op_relation |
10.3855/jidc.11171 JOURNAL OF INFECTION IN DEVELOPING COUNTRIES 1972-2680 J9 J INFECT DEV COUNTR http://hdl.handle.net/11499/32156 13 7 634 639 WOS |
_version_ |
1766400558771470336 |