The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE
INTRODUCTION: Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. METHODOLOGY: The s...
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ftpamukkaleuniv:oai:acikerisim.pau.edu.tr:11499/30111 2023-05-15T16:04:56+02:00 The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE Çalışkan, Ahmet Çopur Çicek, A. Aydogan Ejder, N. Karagöz, A. Kirişci, Ö. Kılıç, S. orcid:0000-0002-1156-3787 2019 application/pdf http://hdl.handle.net/11499/30111 English eng NLM (Medline) 10.3855/jidc.11171 Uluslararası Hakemli Dergi 19722680 (ISSN) http://hdl.handle.net/11499/30111 13 7 634 639 Scopus WOS PubMed info:eu-repo/semantics/openAccess antibiotic sensitivity clonal outbreak PFGE Pulsed Field Gel Electrophoresis Article 2019 ftpamukkaleuniv 2020-11-08T10:04:32Z INTRODUCTION: Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. METHODOLOGY: The study included 153 S. maltophilia strains isolated from clinical samples sent to our hospital laboratory between 1 January 2014 and 30 June 2018. The bacteria were identified and their antibiotic sensitivity was determined using the VITEK-2 automated system. PFGE (Pulsed Field Gel Electrophoresis): The strains isolated from 34 patient clinical samples and from 1 patient bedcover were taken for PFGE examination. RESULTS: The TMP/SXT and levofloxacin sensitivity of 153 S. maltophilia strains was examined. TMP/SXT resistance was determined to be 39% and levofloxacin resistance at 5%. Among 35 S. maltophilia strains, seven genotypes were identified using the PFGE method. While three strains showed a specific genotype profile, the other 32 were determined to consist of four clusters. The cluster rate was therefore 91.4% (32/35). CONCLUSIONS: There was a clonal relationship between the vast majority of the 35 S. maltophilia isolates, which suggests that there was a cross-contamination problem in the hospital. One strain (#4) was identified by dendrogram analysis showed a high rate of similarity to the other strains and was determined to be the common source of the cross-contamination. Copyright (c) 2019 Ahemr Caliksan, Aysegul Copur Cicek, Nebahat Aydogan Ejder, Alper Karagoz, Ozlem Kirisci, Selcuk Kilic. Article in Journal/Newspaper ejder Pamukkale University Repository |
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Pamukkale University Repository |
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ftpamukkaleuniv |
language |
English |
topic |
antibiotic sensitivity clonal outbreak PFGE Pulsed Field Gel Electrophoresis |
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antibiotic sensitivity clonal outbreak PFGE Pulsed Field Gel Electrophoresis Çalışkan, Ahmet Çopur Çicek, A. Aydogan Ejder, N. Karagöz, A. Kirişci, Ö. Kılıç, S. The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE |
topic_facet |
antibiotic sensitivity clonal outbreak PFGE Pulsed Field Gel Electrophoresis |
description |
INTRODUCTION: Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. METHODOLOGY: The study included 153 S. maltophilia strains isolated from clinical samples sent to our hospital laboratory between 1 January 2014 and 30 June 2018. The bacteria were identified and their antibiotic sensitivity was determined using the VITEK-2 automated system. PFGE (Pulsed Field Gel Electrophoresis): The strains isolated from 34 patient clinical samples and from 1 patient bedcover were taken for PFGE examination. RESULTS: The TMP/SXT and levofloxacin sensitivity of 153 S. maltophilia strains was examined. TMP/SXT resistance was determined to be 39% and levofloxacin resistance at 5%. Among 35 S. maltophilia strains, seven genotypes were identified using the PFGE method. While three strains showed a specific genotype profile, the other 32 were determined to consist of four clusters. The cluster rate was therefore 91.4% (32/35). CONCLUSIONS: There was a clonal relationship between the vast majority of the 35 S. maltophilia isolates, which suggests that there was a cross-contamination problem in the hospital. One strain (#4) was identified by dendrogram analysis showed a high rate of similarity to the other strains and was determined to be the common source of the cross-contamination. Copyright (c) 2019 Ahemr Caliksan, Aysegul Copur Cicek, Nebahat Aydogan Ejder, Alper Karagoz, Ozlem Kirisci, Selcuk Kilic. |
author2 |
orcid:0000-0002-1156-3787 |
format |
Article in Journal/Newspaper |
author |
Çalışkan, Ahmet Çopur Çicek, A. Aydogan Ejder, N. Karagöz, A. Kirişci, Ö. Kılıç, S. |
author_facet |
Çalışkan, Ahmet Çopur Çicek, A. Aydogan Ejder, N. Karagöz, A. Kirişci, Ö. Kılıç, S. |
author_sort |
Çalışkan, Ahmet |
title |
The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE |
title_short |
The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE |
title_full |
The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE |
title_fullStr |
The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE |
title_full_unstemmed |
The Antibiotic sensitivity of Stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with PFGE |
title_sort |
antibiotic sensitivity of stenotrophomonas maltophilia in a 5-year period and investigation of clonal outbreak with pfge |
publisher |
NLM (Medline) |
publishDate |
2019 |
url |
http://hdl.handle.net/11499/30111 |
genre |
ejder |
genre_facet |
ejder |
op_relation |
10.3855/jidc.11171 Uluslararası Hakemli Dergi 19722680 (ISSN) http://hdl.handle.net/11499/30111 13 7 634 639 Scopus WOS PubMed |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766400555555487744 |