Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction
We report the complete genome of Thermofilum pendens, a deep-branching, hyperthermophilic member of the order Thermoproteales within the archaeal kingdom Crenarchaeota. T. pendens is a sulfur-dependent, anaerobic heterotroph isolated from a solfatara in Iceland. It is an extracellular commensal, req...
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ftosti:oai:osti.gov:926162 2023-07-30T04:04:26+02:00 Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction Kyrpides, Nikos Anderson, Iain Rodriguez, Jason Susanti, Dwi Porat, Iris Reich, Claudia Ulrich, Luke E. Elkins, James G. Mavromatis, Kostas Lykidis, Athanasios Kim, Edwin Thompson, Linda S. Nolan, Matt Land, Miriam Copeland, Alex Lapidus, Alla Lucas, Susan Detter, Chris Zhulin, Igor B. Olsen, Gary J. Whitman, William Mukhopadhyay, Biswarup Bristow, James 2009-12-16 application/pdf http://www.osti.gov/servlets/purl/926162 https://www.osti.gov/biblio/926162 unknown http://www.osti.gov/servlets/purl/926162 https://www.osti.gov/biblio/926162 59 AMINO ACIDS BINDING ENERGY CARBOHYDRATES ELECTRONS ENERGY SOURCES ENZYMES FERMENTATION FORMATES GENES HYDROGEN NUTRIENTS PARASITES PEPTIDES PHOSPHOTRANSFERASES PROTEINS PURINES SOLFATARAS SULFUR VALENCE 2009 ftosti 2023-07-11T08:45:56Z We report the complete genome of Thermofilum pendens, a deep-branching, hyperthermophilic member of the order Thermoproteales within the archaeal kingdom Crenarchaeota. T. pendens is a sulfur-dependent, anaerobic heterotroph isolated from a solfatara in Iceland. It is an extracellular commensal, requiring an extract of Thermoproteus tenax for growth, and the genome sequence reveals that biosynthetic pathways for purines, most amino acids, and most cofactors are absent. In fact T. pendens has fewer biosynthetic enzymes than obligate intracellular parasites, although it does not display other features common among obligate parasites and thus does not appear to be in the process of becoming a parasite. It appears that T. pendens has adapted to life in an environment rich in nutrients. T. pendens was known to utilize peptides as an energy source, but the genome reveals substantial ability to grow on carbohydrates. T. pendens is the first crenarchaeote and only the second archaeon found to have a transporter of the phosphotransferase system. In addition to fermentation, T. pendens may gain energy from sulfur reduction with hydrogen and formate as electron donors. It may also be capable of sulfur-independent growth on formate with formate hydrogenlyase. Additional novel features are the presence of a monomethylamine:corrinoid methyltransferase, the first time this enzyme has been found outside of Methanosarcinales, and a presenilin-related protein. Predicted highly expressed proteins do not include housekeeping genes, and instead include ABC transporters for carbohydrates and peptides, and CRISPR-associated proteins. Other/Unknown Material Iceland SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
institution |
Open Polar |
collection |
SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
topic |
59 AMINO ACIDS BINDING ENERGY CARBOHYDRATES ELECTRONS ENERGY SOURCES ENZYMES FERMENTATION FORMATES GENES HYDROGEN NUTRIENTS PARASITES PEPTIDES PHOSPHOTRANSFERASES PROTEINS PURINES SOLFATARAS SULFUR VALENCE |
spellingShingle |
59 AMINO ACIDS BINDING ENERGY CARBOHYDRATES ELECTRONS ENERGY SOURCES ENZYMES FERMENTATION FORMATES GENES HYDROGEN NUTRIENTS PARASITES PEPTIDES PHOSPHOTRANSFERASES PROTEINS PURINES SOLFATARAS SULFUR VALENCE Kyrpides, Nikos Anderson, Iain Rodriguez, Jason Susanti, Dwi Porat, Iris Reich, Claudia Ulrich, Luke E. Elkins, James G. Mavromatis, Kostas Lykidis, Athanasios Kim, Edwin Thompson, Linda S. Nolan, Matt Land, Miriam Copeland, Alex Lapidus, Alla Lucas, Susan Detter, Chris Zhulin, Igor B. Olsen, Gary J. Whitman, William Mukhopadhyay, Biswarup Bristow, James Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction |
topic_facet |
59 AMINO ACIDS BINDING ENERGY CARBOHYDRATES ELECTRONS ENERGY SOURCES ENZYMES FERMENTATION FORMATES GENES HYDROGEN NUTRIENTS PARASITES PEPTIDES PHOSPHOTRANSFERASES PROTEINS PURINES SOLFATARAS SULFUR VALENCE |
description |
We report the complete genome of Thermofilum pendens, a deep-branching, hyperthermophilic member of the order Thermoproteales within the archaeal kingdom Crenarchaeota. T. pendens is a sulfur-dependent, anaerobic heterotroph isolated from a solfatara in Iceland. It is an extracellular commensal, requiring an extract of Thermoproteus tenax for growth, and the genome sequence reveals that biosynthetic pathways for purines, most amino acids, and most cofactors are absent. In fact T. pendens has fewer biosynthetic enzymes than obligate intracellular parasites, although it does not display other features common among obligate parasites and thus does not appear to be in the process of becoming a parasite. It appears that T. pendens has adapted to life in an environment rich in nutrients. T. pendens was known to utilize peptides as an energy source, but the genome reveals substantial ability to grow on carbohydrates. T. pendens is the first crenarchaeote and only the second archaeon found to have a transporter of the phosphotransferase system. In addition to fermentation, T. pendens may gain energy from sulfur reduction with hydrogen and formate as electron donors. It may also be capable of sulfur-independent growth on formate with formate hydrogenlyase. Additional novel features are the presence of a monomethylamine:corrinoid methyltransferase, the first time this enzyme has been found outside of Methanosarcinales, and a presenilin-related protein. Predicted highly expressed proteins do not include housekeeping genes, and instead include ABC transporters for carbohydrates and peptides, and CRISPR-associated proteins. |
author |
Kyrpides, Nikos Anderson, Iain Rodriguez, Jason Susanti, Dwi Porat, Iris Reich, Claudia Ulrich, Luke E. Elkins, James G. Mavromatis, Kostas Lykidis, Athanasios Kim, Edwin Thompson, Linda S. Nolan, Matt Land, Miriam Copeland, Alex Lapidus, Alla Lucas, Susan Detter, Chris Zhulin, Igor B. Olsen, Gary J. Whitman, William Mukhopadhyay, Biswarup Bristow, James |
author_facet |
Kyrpides, Nikos Anderson, Iain Rodriguez, Jason Susanti, Dwi Porat, Iris Reich, Claudia Ulrich, Luke E. Elkins, James G. Mavromatis, Kostas Lykidis, Athanasios Kim, Edwin Thompson, Linda S. Nolan, Matt Land, Miriam Copeland, Alex Lapidus, Alla Lucas, Susan Detter, Chris Zhulin, Igor B. Olsen, Gary J. Whitman, William Mukhopadhyay, Biswarup Bristow, James |
author_sort |
Kyrpides, Nikos |
title |
Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction |
title_short |
Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction |
title_full |
Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction |
title_fullStr |
Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction |
title_full_unstemmed |
Genome sequence of Thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction |
title_sort |
genome sequence of thermofilum pendens reveals an exceptional loss of biosynthetic pathways without genome reduction |
publishDate |
2009 |
url |
http://www.osti.gov/servlets/purl/926162 https://www.osti.gov/biblio/926162 |
genre |
Iceland |
genre_facet |
Iceland |
op_relation |
http://www.osti.gov/servlets/purl/926162 https://www.osti.gov/biblio/926162 |
_version_ |
1772815890733072384 |