Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health
Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status...
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ftosti:oai:osti.gov:1905046 2023-07-30T04:06:31+02:00 Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. 2023-05-18 application/pdf http://www.osti.gov/servlets/purl/1905046 https://www.osti.gov/biblio/1905046 https://doi.org/10.1038/s41598-022-04846-3 unknown http://www.osti.gov/servlets/purl/1905046 https://www.osti.gov/biblio/1905046 https://doi.org/10.1038/s41598-022-04846-3 doi:10.1038/s41598-022-04846-3 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1038/s41598-022-04846-3 2023-07-11T10:17:01Z Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status was identified for 64/140 (45.7%); 49 (35.0%) were positive by dark-field microscopy, 60 (42.9%) by culture, 63 (45.0%) by fluorescent antibody testing, and 61 (43.6%) by real-time polymerase chain reaction (rtPCR). Molecular typing indicated that 48 isolates were L. borgpetersenii and 3 were L. kirschneri; the remaining nine comprised mixed species. In the single culture-negative sample that was rtPCR positive, genotyping directly from the kidney identified L. interrogans. Serotyping of L. borgpetersenii isolates identified serogroup Ballum and L. kirschneri isolates as serogroup Icterohaemorrhagiae. These results demonstrate that rodents are significant Leptospira carriers and adds to understanding the ecoepidemiology of leptospirosis in USVI. Other/Unknown Material Rattus rattus SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Scientific Reports 12 1 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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59 BASIC BIOLOGICAL SCIENCES |
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59 BASIC BIOLOGICAL SCIENCES Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the genus Leptospira. We sought to determine if rodents in U.S. Virgin Islands (USVI) are carriers of Leptospira. In total, 140 rodents were sampled, including 112 Mus musculus and 28 Rattus rattus. A positive carrier status was identified for 64/140 (45.7%); 49 (35.0%) were positive by dark-field microscopy, 60 (42.9%) by culture, 63 (45.0%) by fluorescent antibody testing, and 61 (43.6%) by real-time polymerase chain reaction (rtPCR). Molecular typing indicated that 48 isolates were L. borgpetersenii and 3 were L. kirschneri; the remaining nine comprised mixed species. In the single culture-negative sample that was rtPCR positive, genotyping directly from the kidney identified L. interrogans. Serotyping of L. borgpetersenii isolates identified serogroup Ballum and L. kirschneri isolates as serogroup Icterohaemorrhagiae. These results demonstrate that rodents are significant Leptospira carriers and adds to understanding the ecoepidemiology of leptospirosis in USVI. |
author |
Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. |
author_facet |
Hamond, Camila Browne, A. Springer de Wilde, Leah H. Hornsby, Richard L. LeCount, Karen Anderson, Tammy Stuber, Tod Cranford, Hannah M. Browne, Stephanie K. Blanchard, Gerard Horner, David Taylor, Marissa L. Evans, Michael Angeli, Nicole F. Roth, Joseph Bisgard, Kristine M. Salzer, Johanna S. Schafer, Ilana J. Ellis, Brett R. Alt, David P. Schlater, Linda Nally, Jarlath E. Ellis, Esther M. |
author_sort |
Hamond, Camila |
title |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_short |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_full |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_fullStr |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_full_unstemmed |
Assessing rodents as carriers of pathogenic Leptospira species in the U.S. Virgin Islands and their risk to animal and public health |
title_sort |
assessing rodents as carriers of pathogenic leptospira species in the u.s. virgin islands and their risk to animal and public health |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1905046 https://www.osti.gov/biblio/1905046 https://doi.org/10.1038/s41598-022-04846-3 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
http://www.osti.gov/servlets/purl/1905046 https://www.osti.gov/biblio/1905046 https://doi.org/10.1038/s41598-022-04846-3 doi:10.1038/s41598-022-04846-3 |
op_doi |
https://doi.org/10.1038/s41598-022-04846-3 |
container_title |
Scientific Reports |
container_volume |
12 |
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1 |
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1772819162381418496 |