Remarkably coherent population structure for a dominant Antarctic Chlorobium species
Background: In Antarctica, summer sunlight enables phototrophic microorganisms to drive primary production, thereby “feeding” ecosystems to enable their persistence through the long, dark winter months. In Ace Lake, a stratified marine-derived system in the Vestfold Hills of East Antarctica, a Chlor...
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Online Access: | http://www.osti.gov/servlets/purl/1904122 https://www.osti.gov/biblio/1904122 https://doi.org/10.1186/s40168-021-01173-z |
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ftosti:oai:osti.gov:1904122 2023-07-30T03:59:09+02:00 Remarkably coherent population structure for a dominant Antarctic Chlorobium species Panwar, Pratibha Allen, Michelle A. Williams, Timothy J. Haque, Sabrina Brazendale, Sarah Hancock, Alyce M. Paez-Espino, David Cavicchioli, Ricardo 2023-07-10 application/pdf http://www.osti.gov/servlets/purl/1904122 https://www.osti.gov/biblio/1904122 https://doi.org/10.1186/s40168-021-01173-z unknown http://www.osti.gov/servlets/purl/1904122 https://www.osti.gov/biblio/1904122 https://doi.org/10.1186/s40168-021-01173-z doi:10.1186/s40168-021-01173-z 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1186/s40168-021-01173-z 2023-07-11T10:16:52Z Background: In Antarctica, summer sunlight enables phototrophic microorganisms to drive primary production, thereby “feeding” ecosystems to enable their persistence through the long, dark winter months. In Ace Lake, a stratified marine-derived system in the Vestfold Hills of East Antarctica, a Chlorobium species of green sulphur bacteria (GSB) is the dominant phototroph, although its seasonal abundance changes more than 100-fold. Here, we analysed 413 Gb of Antarctic metagenome data including 59 Chlorobium metagenome-assembled genomes (MAGs) from Ace Lake and nearby stratified marine basins to determine how genome variation and population structure across a 7-year period impacted ecosystem function. Results: A single species, Candidatus Chlorobium antarcticum (most similar to Chlorobium phaeovibrioides DSM265) prevails in all three aquatic systems and harbours very little genomic variation (≥ 99% average nucleotide identity). A notable feature of variation that did exist related to the genomic capacity to biosynthesize cobalamin. The abundance of phylotypes with this capacity changed seasonally ~ 2-fold, consistent with the population balancing the value of a bolstered photosynthetic capacity in summer against an energetic cost in winter. The very high GSB concentration (> 10 8 cells ml –1 in Ace Lake) and seasonal cycle of cell lysis likely make Ca. Chlorobium antarcticum a major provider of cobalamin to the food web. Analysis of Ca. Chlorobium antarcticum viruses revealed the species to be infected by generalist (rather than specialist) viruses with a broad host range (e.g., infecting Gammaproteobacteria) that were present in diverse Antarctic lakes. The marked seasonal decrease in Ca. Chlorobium antarcticum abundance may restrict specialist viruses from establishing effective lifecycles, whereas generalist viruses may augment their proliferation using other hosts. Conclusion: The factors shaping Antarctic microbial communities are gradually being defined. In addition to the cold, the annual variation in ... Other/Unknown Material Antarc* Antarctic Antarctica East Antarctica SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Antarctic East Antarctica Vestfold Vestfold Hills Microbiome 9 1 |
institution |
Open Polar |
collection |
SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
topic |
59 BASIC BIOLOGICAL SCIENCES |
spellingShingle |
59 BASIC BIOLOGICAL SCIENCES Panwar, Pratibha Allen, Michelle A. Williams, Timothy J. Haque, Sabrina Brazendale, Sarah Hancock, Alyce M. Paez-Espino, David Cavicchioli, Ricardo Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Background: In Antarctica, summer sunlight enables phototrophic microorganisms to drive primary production, thereby “feeding” ecosystems to enable their persistence through the long, dark winter months. In Ace Lake, a stratified marine-derived system in the Vestfold Hills of East Antarctica, a Chlorobium species of green sulphur bacteria (GSB) is the dominant phototroph, although its seasonal abundance changes more than 100-fold. Here, we analysed 413 Gb of Antarctic metagenome data including 59 Chlorobium metagenome-assembled genomes (MAGs) from Ace Lake and nearby stratified marine basins to determine how genome variation and population structure across a 7-year period impacted ecosystem function. Results: A single species, Candidatus Chlorobium antarcticum (most similar to Chlorobium phaeovibrioides DSM265) prevails in all three aquatic systems and harbours very little genomic variation (≥ 99% average nucleotide identity). A notable feature of variation that did exist related to the genomic capacity to biosynthesize cobalamin. The abundance of phylotypes with this capacity changed seasonally ~ 2-fold, consistent with the population balancing the value of a bolstered photosynthetic capacity in summer against an energetic cost in winter. The very high GSB concentration (> 10 8 cells ml –1 in Ace Lake) and seasonal cycle of cell lysis likely make Ca. Chlorobium antarcticum a major provider of cobalamin to the food web. Analysis of Ca. Chlorobium antarcticum viruses revealed the species to be infected by generalist (rather than specialist) viruses with a broad host range (e.g., infecting Gammaproteobacteria) that were present in diverse Antarctic lakes. The marked seasonal decrease in Ca. Chlorobium antarcticum abundance may restrict specialist viruses from establishing effective lifecycles, whereas generalist viruses may augment their proliferation using other hosts. Conclusion: The factors shaping Antarctic microbial communities are gradually being defined. In addition to the cold, the annual variation in ... |
author |
Panwar, Pratibha Allen, Michelle A. Williams, Timothy J. Haque, Sabrina Brazendale, Sarah Hancock, Alyce M. Paez-Espino, David Cavicchioli, Ricardo |
author_facet |
Panwar, Pratibha Allen, Michelle A. Williams, Timothy J. Haque, Sabrina Brazendale, Sarah Hancock, Alyce M. Paez-Espino, David Cavicchioli, Ricardo |
author_sort |
Panwar, Pratibha |
title |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_short |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_full |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_fullStr |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_full_unstemmed |
Remarkably coherent population structure for a dominant Antarctic Chlorobium species |
title_sort |
remarkably coherent population structure for a dominant antarctic chlorobium species |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1904122 https://www.osti.gov/biblio/1904122 https://doi.org/10.1186/s40168-021-01173-z |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) |
geographic |
Ace Lake Antarctic East Antarctica Vestfold Vestfold Hills |
geographic_facet |
Ace Lake Antarctic East Antarctica Vestfold Vestfold Hills |
genre |
Antarc* Antarctic Antarctica East Antarctica |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica |
op_relation |
http://www.osti.gov/servlets/purl/1904122 https://www.osti.gov/biblio/1904122 https://doi.org/10.1186/s40168-021-01173-z doi:10.1186/s40168-021-01173-z |
op_doi |
https://doi.org/10.1186/s40168-021-01173-z |
container_title |
Microbiome |
container_volume |
9 |
container_issue |
1 |
_version_ |
1772809866124984320 |