Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of...
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ftosti:oai:osti.gov:1878507 2023-07-30T04:01:03+02:00 Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie 2023-07-10 application/pdf http://www.osti.gov/servlets/purl/1878507 https://www.osti.gov/biblio/1878507 https://doi.org/10.1038/s42003-022-03461-2 unknown http://www.osti.gov/servlets/purl/1878507 https://www.osti.gov/biblio/1878507 https://doi.org/10.1038/s42003-022-03461-2 doi:10.1038/s42003-022-03461-2 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1038/s42003-022-03461-2 2023-07-11T10:13:45Z Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na + -H + antiporters and Na + -Pi symporters as salinity decreases, but the K + channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. Other/Unknown Material Arctic Sea ice SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic Communications Biology 5 1 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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59 BASIC BIOLOGICAL SCIENCES |
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59 BASIC BIOLOGICAL SCIENCES Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Little is known at the transcriptional level about microbial eukaryotic adaptations to short-term salinity change. Arctic microalgae are exposed to low salinity due to sea-ice melt and higher salinity with brine channel formation during freeze-up. Here, we investigate the transcriptional response of an ice-associated microalgae over salinities from 45 to 8. Our results show a bracketed response of differential gene expression when the cultures were exposed to progressively decreasing salinity. Key genes associated with salinity changes were involved in specific metabolic pathways, transcription factors and regulators, protein kinases, carbohydrate active enzymes, and inorganic ion transporters. The pelagophyte seemed to use a strategy involving overexpression of Na + -H + antiporters and Na + -Pi symporters as salinity decreases, but the K + channel complex at higher salinities. Specific adaptation to cold saline arctic conditions was seen with differential expression of several antifreeze proteins, an ice-binding protein and an acyl-esterase involved in cold adaptation. |
author |
Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie |
author_facet |
Freyria, Nastasia J. Kuo, Alan Chovatia, Mansi Johnson, Jenifer Lipzen, Anna Barry, Kerrie W. Grigoriev, Igor V. Lovejoy, Connie |
author_sort |
Freyria, Nastasia J. |
title |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_short |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_fullStr |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_full_unstemmed |
Salinity tolerance mechanisms of an Arctic Pelagophyte using comparative transcriptomic and gene expression analysis |
title_sort |
salinity tolerance mechanisms of an arctic pelagophyte using comparative transcriptomic and gene expression analysis |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1878507 https://www.osti.gov/biblio/1878507 https://doi.org/10.1038/s42003-022-03461-2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Sea ice |
genre_facet |
Arctic Sea ice |
op_relation |
http://www.osti.gov/servlets/purl/1878507 https://www.osti.gov/biblio/1878507 https://doi.org/10.1038/s42003-022-03461-2 doi:10.1038/s42003-022-03461-2 |
op_doi |
https://doi.org/10.1038/s42003-022-03461-2 |
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Communications Biology |
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5 |
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1 |
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1772811764830830592 |