Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations
Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in peatlands underlain with permafrost, little is known...
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Online Access: | http://www.osti.gov/servlets/purl/1845648 https://www.osti.gov/biblio/1845648 https://doi.org/10.1186/s40168-021-01156-0 |
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ftosti:oai:osti.gov:1845648 2023-07-30T04:06:19+02:00 Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. 2023-07-04 application/pdf http://www.osti.gov/servlets/purl/1845648 https://www.osti.gov/biblio/1845648 https://doi.org/10.1186/s40168-021-01156-0 unknown http://www.osti.gov/servlets/purl/1845648 https://www.osti.gov/biblio/1845648 https://doi.org/10.1186/s40168-021-01156-0 doi:10.1186/s40168-021-01156-0 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1186/s40168-021-01156-0 2023-07-11T10:10:13Z Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in peatlands underlain with permafrost, little is known about viruses in other peatlands. More generally, soil viral biogeography and its potential drivers are poorly understood at both local and global scales. Here, 87 metagenomes and five viral size-fraction metagenomes (viromes) from a boreal peatland in northern Minnesota (the SPRUCE whole-ecosystem warming experiment and surrounding bog) were analyzed for dsDNA viral community ecological patterns, and the recovered viral populations (vOTUs) were compared with our curated PIGEON database of 266,125 vOTUs from diverse ecosystems. Within the SPRUCE experiment, viral community composition was significantly correlated with peat depth, water content, and carbon chemistry, including CH 4 and CO 2 concentrations, but not with temperature during the first 2 years of warming treatments. Peat vOTUs with aquatic-like signatures (shared predicted protein content with marine and/or freshwater vOTUs) were significantly enriched in more waterlogged surface peat depths. Predicted host ranges for SPRUCE vOTUs were relatively narrow, generally within a single bacterial genus. Of the 4326 SPRUCE vOTUs, 164 were previously detected in other soils, mostly peatlands. None of the previously identified 202,371 marine and freshwater vOTUs in our PIGEON database were detected in SPRUCE peat, but 0.4% of 80,714 viral clusters (VCs, grouped by predicted protein content) were shared between soil and aquatic environments. On a per-sample basis, vOTU recovery was 32 times higher from viromes compared with total metagenomes. Results suggest strong viral “species” boundaries between terrestrial and aquatic ecosystems and to some extent between peat and other soils, with differences less pronounced at higher taxonomic levels. The significant ... Other/Unknown Material permafrost SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Microbiome 9 1 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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ftosti |
language |
unknown |
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59 BASIC BIOLOGICAL SCIENCES |
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59 BASIC BIOLOGICAL SCIENCES ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Peatlands are expected to experience sustained yet fluctuating higher temperatures due to climate change, leading to increased microbial activity and greenhouse gas emissions. Despite mounting evidence for viral contributions to these processes in peatlands underlain with permafrost, little is known about viruses in other peatlands. More generally, soil viral biogeography and its potential drivers are poorly understood at both local and global scales. Here, 87 metagenomes and five viral size-fraction metagenomes (viromes) from a boreal peatland in northern Minnesota (the SPRUCE whole-ecosystem warming experiment and surrounding bog) were analyzed for dsDNA viral community ecological patterns, and the recovered viral populations (vOTUs) were compared with our curated PIGEON database of 266,125 vOTUs from diverse ecosystems. Within the SPRUCE experiment, viral community composition was significantly correlated with peat depth, water content, and carbon chemistry, including CH 4 and CO 2 concentrations, but not with temperature during the first 2 years of warming treatments. Peat vOTUs with aquatic-like signatures (shared predicted protein content with marine and/or freshwater vOTUs) were significantly enriched in more waterlogged surface peat depths. Predicted host ranges for SPRUCE vOTUs were relatively narrow, generally within a single bacterial genus. Of the 4326 SPRUCE vOTUs, 164 were previously detected in other soils, mostly peatlands. None of the previously identified 202,371 marine and freshwater vOTUs in our PIGEON database were detected in SPRUCE peat, but 0.4% of 80,714 viral clusters (VCs, grouped by predicted protein content) were shared between soil and aquatic environments. On a per-sample basis, vOTU recovery was 32 times higher from viromes compared with total metagenomes. Results suggest strong viral “species” boundaries between terrestrial and aquatic ecosystems and to some extent between peat and other soils, with differences less pronounced at higher taxonomic levels. The significant ... |
author |
ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. |
author_facet |
ter Horst, Anneliek M. Santos-Medellín, Christian Sorensen, Jackson W. Zinke, Laura A. Wilson, Rachel M. Johnston, Eric R. Trubl, Gareth Pett-Ridge, Jennifer Blazewicz, Steven J. Hanson, Paul J. Chanton, Jeffrey P. Schadt, Christopher W. Kostka, Joel E. Emerson, Joanne B. |
author_sort |
ter Horst, Anneliek M. |
title |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_short |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_full |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_fullStr |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_full_unstemmed |
Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
title_sort |
minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1845648 https://www.osti.gov/biblio/1845648 https://doi.org/10.1186/s40168-021-01156-0 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
http://www.osti.gov/servlets/purl/1845648 https://www.osti.gov/biblio/1845648 https://doi.org/10.1186/s40168-021-01156-0 doi:10.1186/s40168-021-01156-0 |
op_doi |
https://doi.org/10.1186/s40168-021-01156-0 |
container_title |
Microbiome |
container_volume |
9 |
container_issue |
1 |
_version_ |
1772818859744559104 |