Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils
Rising temperatures in the Arctic have led to the thawing of tundra soils, which is rapidly changing terrain, hydrology, and plant and microbial communities, causing hotspots of biogeochemical activity across the landscape. Despite this, little is known about how nutrient-rich low molecular weight d...
Published in: | Soil Systems |
---|---|
Main Authors: | , , , , , |
Language: | unknown |
Published: |
2021
|
Subjects: | |
Online Access: | http://www.osti.gov/servlets/purl/1817588 https://www.osti.gov/biblio/1817588 https://doi.org/10.3390/soilsystems5010010 |
id |
ftosti:oai:osti.gov:1817588 |
---|---|
record_format |
openpolar |
spelling |
ftosti:oai:osti.gov:1817588 2023-07-30T04:01:14+02:00 Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils Ladd, Mallory P. Reeves, David T. Poudel, Suresh Iversen, Colleen M. Wullschleger, Stan D. Hettich, Robert L. 2021-09-15 application/pdf http://www.osti.gov/servlets/purl/1817588 https://www.osti.gov/biblio/1817588 https://doi.org/10.3390/soilsystems5010010 unknown http://www.osti.gov/servlets/purl/1817588 https://www.osti.gov/biblio/1817588 https://doi.org/10.3390/soilsystems5010010 doi:10.3390/soilsystems5010010 58 GEOSCIENCES 59 BASIC BIOLOGICAL SCIENCES 2021 ftosti https://doi.org/10.3390/soilsystems5010010 2023-07-11T10:06:39Z Rising temperatures in the Arctic have led to the thawing of tundra soils, which is rapidly changing terrain, hydrology, and plant and microbial communities, causing hotspots of biogeochemical activity across the landscape. Despite this, little is known about how nutrient-rich low molecular weight dissolved organic matter (LMW DOM) varies within and across tundra ecosystems. Using a high-resolution nano-liquid chromatography-mass spectrometry (LC/MS) approach, we characterized the composition and availability of LMW DOM from high-centered polygons (HCP) and low-centered polygons (LCP) with Eriophorum angustifolium or Carex aquatilis as the dominant vegetation. Over 3000 unique features (i.e., discrete mass/charge ions) were detected; 521 were identified as differentially abundant between polygonal types and 217 were putatively annotated using high mass accuracy MS data. While polygon type was a strong predictor of LMW DOM composition and availability, vegetation and soil depth were also important drivers. Extensive evidence was found for enhanced microbial processing at the LCP sites, which were dominated by Carex plant species. We detected significant differences between polygon types with varying aboveground landscape features or properties, and hotspots of biogeochemical activity, indicating LMW DOM, as quantified by untargeted exometabolomics, provides a window into the dynamic complex interactions between landscape topography, vegetation, and organic matter cycling in Arctic polygonal tundra soils. Other/Unknown Material Arctic Carex aquatilis Eriophorum Tundra SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic Soil Systems 5 1 10 |
institution |
Open Polar |
collection |
SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
topic |
58 GEOSCIENCES 59 BASIC BIOLOGICAL SCIENCES |
spellingShingle |
58 GEOSCIENCES 59 BASIC BIOLOGICAL SCIENCES Ladd, Mallory P. Reeves, David T. Poudel, Suresh Iversen, Colleen M. Wullschleger, Stan D. Hettich, Robert L. Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
topic_facet |
58 GEOSCIENCES 59 BASIC BIOLOGICAL SCIENCES |
description |
Rising temperatures in the Arctic have led to the thawing of tundra soils, which is rapidly changing terrain, hydrology, and plant and microbial communities, causing hotspots of biogeochemical activity across the landscape. Despite this, little is known about how nutrient-rich low molecular weight dissolved organic matter (LMW DOM) varies within and across tundra ecosystems. Using a high-resolution nano-liquid chromatography-mass spectrometry (LC/MS) approach, we characterized the composition and availability of LMW DOM from high-centered polygons (HCP) and low-centered polygons (LCP) with Eriophorum angustifolium or Carex aquatilis as the dominant vegetation. Over 3000 unique features (i.e., discrete mass/charge ions) were detected; 521 were identified as differentially abundant between polygonal types and 217 were putatively annotated using high mass accuracy MS data. While polygon type was a strong predictor of LMW DOM composition and availability, vegetation and soil depth were also important drivers. Extensive evidence was found for enhanced microbial processing at the LCP sites, which were dominated by Carex plant species. We detected significant differences between polygon types with varying aboveground landscape features or properties, and hotspots of biogeochemical activity, indicating LMW DOM, as quantified by untargeted exometabolomics, provides a window into the dynamic complex interactions between landscape topography, vegetation, and organic matter cycling in Arctic polygonal tundra soils. |
author |
Ladd, Mallory P. Reeves, David T. Poudel, Suresh Iversen, Colleen M. Wullschleger, Stan D. Hettich, Robert L. |
author_facet |
Ladd, Mallory P. Reeves, David T. Poudel, Suresh Iversen, Colleen M. Wullschleger, Stan D. Hettich, Robert L. |
author_sort |
Ladd, Mallory P. |
title |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_short |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_full |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_fullStr |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_full_unstemmed |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_sort |
untargeted exometabolomics provides a powerful approach to investigate biogeochemical hotspots with vegetation and polygon type in arctic tundra soils |
publishDate |
2021 |
url |
http://www.osti.gov/servlets/purl/1817588 https://www.osti.gov/biblio/1817588 https://doi.org/10.3390/soilsystems5010010 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Carex aquatilis Eriophorum Tundra |
genre_facet |
Arctic Carex aquatilis Eriophorum Tundra |
op_relation |
http://www.osti.gov/servlets/purl/1817588 https://www.osti.gov/biblio/1817588 https://doi.org/10.3390/soilsystems5010010 doi:10.3390/soilsystems5010010 |
op_doi |
https://doi.org/10.3390/soilsystems5010010 |
container_title |
Soil Systems |
container_volume |
5 |
container_issue |
1 |
container_start_page |
10 |
_version_ |
1772811979773181952 |