The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges
Members of the genus Exiguobacterium are found in diverse environments from marine and fresh waters to permafrost and hot springs. As such, they can grow at a wide range of temperature, pH, salinity, and heavy-metal concentrations. Here we propose Exiguobacterium pavilionensis sp. nov. strain RW2 us...
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ftosti:oai:osti.gov:1774145 2023-07-30T04:06:19+02:00 The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges White, Richard Allen Soles, Sarah A. Gavelis, Greg Gosselin, Emma Slater, Greg F. Lim, Darlene S. Leander, Brian Suttle, Curtis A. 2023-07-03 application/pdf http://www.osti.gov/servlets/purl/1774145 https://www.osti.gov/biblio/1774145 https://doi.org/10.3389/fmicb.2018.03189 unknown http://www.osti.gov/servlets/purl/1774145 https://www.osti.gov/biblio/1774145 https://doi.org/10.3389/fmicb.2018.03189 doi:10.3389/fmicb.2018.03189 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.3389/fmicb.2018.03189 2023-07-11T10:02:24Z Members of the genus Exiguobacterium are found in diverse environments from marine and fresh waters to permafrost and hot springs. As such, they can grow at a wide range of temperature, pH, salinity, and heavy-metal concentrations. Here we propose Exiguobacterium pavilionensis sp. nov. strain RW2 using phylogenetic, biochemical, and genome analyses. Isolated from a cold freshwater microbialite in Pavilion Lake, British Columbia, E. pavilionensis can grow between 4 and 50 °C, the broadest temperature range reported for the genus. The three Mbp circular genome includes a large set of core genes associated with stress responses to temperature, salinity and heavy metals that are conserved among all sequenced strains of Exiguobacterium spp., and which may explain their distribution across such a wide range of ecosystems. We also nominate potential pathways involved in carbonate precipitation (and therefore microbialite formation), as well as carotenoid biosynthesis that could lend the characteristic orange color to members of the genus. The unexpectedly high thermal tolerance of this cold-water bacterium, as well as its latent flagellum synthesis operons, suggest that this species may have had its evolutionary origins in a much warmer environment in which motility was important. These results provide further insight into the potential of Exiguobacterium to exploit a wide range of environmental conditions. Other/Unknown Material permafrost SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Frontiers in Microbiology 9 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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59 BASIC BIOLOGICAL SCIENCES |
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59 BASIC BIOLOGICAL SCIENCES White, Richard Allen Soles, Sarah A. Gavelis, Greg Gosselin, Emma Slater, Greg F. Lim, Darlene S. Leander, Brian Suttle, Curtis A. The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Members of the genus Exiguobacterium are found in diverse environments from marine and fresh waters to permafrost and hot springs. As such, they can grow at a wide range of temperature, pH, salinity, and heavy-metal concentrations. Here we propose Exiguobacterium pavilionensis sp. nov. strain RW2 using phylogenetic, biochemical, and genome analyses. Isolated from a cold freshwater microbialite in Pavilion Lake, British Columbia, E. pavilionensis can grow between 4 and 50 °C, the broadest temperature range reported for the genus. The three Mbp circular genome includes a large set of core genes associated with stress responses to temperature, salinity and heavy metals that are conserved among all sequenced strains of Exiguobacterium spp., and which may explain their distribution across such a wide range of ecosystems. We also nominate potential pathways involved in carbonate precipitation (and therefore microbialite formation), as well as carotenoid biosynthesis that could lend the characteristic orange color to members of the genus. The unexpectedly high thermal tolerance of this cold-water bacterium, as well as its latent flagellum synthesis operons, suggest that this species may have had its evolutionary origins in a much warmer environment in which motility was important. These results provide further insight into the potential of Exiguobacterium to exploit a wide range of environmental conditions. |
author |
White, Richard Allen Soles, Sarah A. Gavelis, Greg Gosselin, Emma Slater, Greg F. Lim, Darlene S. Leander, Brian Suttle, Curtis A. |
author_facet |
White, Richard Allen Soles, Sarah A. Gavelis, Greg Gosselin, Emma Slater, Greg F. Lim, Darlene S. Leander, Brian Suttle, Curtis A. |
author_sort |
White, Richard Allen |
title |
The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges |
title_short |
The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges |
title_full |
The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges |
title_fullStr |
The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges |
title_full_unstemmed |
The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Exiguobacterium chiriqhucha Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges |
title_sort |
complete genome and physiological analysis of the eurythermal firmicute exiguobacterium chiriqhucha strain rw2 isolated from a freshwater microbialite, widely adaptable to broad thermal, ph, and salinity ranges |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1774145 https://www.osti.gov/biblio/1774145 https://doi.org/10.3389/fmicb.2018.03189 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
http://www.osti.gov/servlets/purl/1774145 https://www.osti.gov/biblio/1774145 https://doi.org/10.3389/fmicb.2018.03189 doi:10.3389/fmicb.2018.03189 |
op_doi |
https://doi.org/10.3389/fmicb.2018.03189 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
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1772818841824395264 |