Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity
The Antarctic green alga Chlamydomonas sp. UWO 241 (UWO 241) is adapted to permanent low temperatures, hypersalinity, and extreme shade. One of the most striking phenotypes of UWO 241 is an altered PSI organization and constitutive PSI cyclic electron flow (CEF). To date, little attention has been p...
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ftosti:oai:osti.gov:1657213 2023-07-30T03:58:50+02:00 Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity Kalra, Isha Wang, Xin Cvetkovska, Marina Jeong, Jooyeon McHargue, William Zhang, Ru Hüner, Norman Yuan, Joshua S. Morgan-Kiss, Rachael 2022-05-23 application/pdf http://www.osti.gov/servlets/purl/1657213 https://www.osti.gov/biblio/1657213 https://doi.org/10.1104/pp.19.01280 unknown http://www.osti.gov/servlets/purl/1657213 https://www.osti.gov/biblio/1657213 https://doi.org/10.1104/pp.19.01280 doi:10.1104/pp.19.01280 79 ASTRONOMY AND ASTROPHYSICS 2022 ftosti https://doi.org/10.1104/pp.19.01280 2023-07-11T09:46:25Z The Antarctic green alga Chlamydomonas sp. UWO 241 (UWO 241) is adapted to permanent low temperatures, hypersalinity, and extreme shade. One of the most striking phenotypes of UWO 241 is an altered PSI organization and constitutive PSI cyclic electron flow (CEF). To date, little attention has been paid to CEF during long-term stress acclimation, and the consequences of sustained CEF in UWO 241 are not known. In this study, we combined photobiology, proteomics, and metabolomics to understand the underlying role of sustained CEF in high-salinity stress acclimation. High salt-grown UWO 241 exhibited increased thylakoid proton motive flux and an increased capacity for nonphotochemical quenching. Under high salt, a significant proportion of the up-regulafted enzymes were associated with the Calvin-Benson-Bassham cycle, carbon storage metabolism, and protein translation. Two key enzymes of the shikimate pathway, 3-deoxy-d-arabinoheptulosonate 7-phosphate synthase and chorismate synthase, were also up-regulated, as well as indole-3-glycerol phosphate synthase, an enzyme involved in the biosynthesis of l-Trp and indole acetic acid. In addition, several compatible solutes (glycerol, Pro, and Suc) accumulated to high levels in high salt-grown UWO 241 cultures. We suggest that UWO 241 maintains constitutively high CEF through the associated PSI-cytochrome b 6 f supercomplex to support robust growth and strong photosynthetic capacity under a constant growth regime of low temperatures and high salinity. Other/Unknown Material Antarc* Antarctic SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Antarctic The Antarctic Psi ENVELOPE(-63.000,-63.000,-64.300,-64.300) Calvin ENVELOPE(165.100,165.100,-71.283,-71.283) Plant Physiology 183 2 588 601 |
institution |
Open Polar |
collection |
SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
topic |
79 ASTRONOMY AND ASTROPHYSICS |
spellingShingle |
79 ASTRONOMY AND ASTROPHYSICS Kalra, Isha Wang, Xin Cvetkovska, Marina Jeong, Jooyeon McHargue, William Zhang, Ru Hüner, Norman Yuan, Joshua S. Morgan-Kiss, Rachael Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity |
topic_facet |
79 ASTRONOMY AND ASTROPHYSICS |
description |
The Antarctic green alga Chlamydomonas sp. UWO 241 (UWO 241) is adapted to permanent low temperatures, hypersalinity, and extreme shade. One of the most striking phenotypes of UWO 241 is an altered PSI organization and constitutive PSI cyclic electron flow (CEF). To date, little attention has been paid to CEF during long-term stress acclimation, and the consequences of sustained CEF in UWO 241 are not known. In this study, we combined photobiology, proteomics, and metabolomics to understand the underlying role of sustained CEF in high-salinity stress acclimation. High salt-grown UWO 241 exhibited increased thylakoid proton motive flux and an increased capacity for nonphotochemical quenching. Under high salt, a significant proportion of the up-regulafted enzymes were associated with the Calvin-Benson-Bassham cycle, carbon storage metabolism, and protein translation. Two key enzymes of the shikimate pathway, 3-deoxy-d-arabinoheptulosonate 7-phosphate synthase and chorismate synthase, were also up-regulated, as well as indole-3-glycerol phosphate synthase, an enzyme involved in the biosynthesis of l-Trp and indole acetic acid. In addition, several compatible solutes (glycerol, Pro, and Suc) accumulated to high levels in high salt-grown UWO 241 cultures. We suggest that UWO 241 maintains constitutively high CEF through the associated PSI-cytochrome b 6 f supercomplex to support robust growth and strong photosynthetic capacity under a constant growth regime of low temperatures and high salinity. |
author |
Kalra, Isha Wang, Xin Cvetkovska, Marina Jeong, Jooyeon McHargue, William Zhang, Ru Hüner, Norman Yuan, Joshua S. Morgan-Kiss, Rachael |
author_facet |
Kalra, Isha Wang, Xin Cvetkovska, Marina Jeong, Jooyeon McHargue, William Zhang, Ru Hüner, Norman Yuan, Joshua S. Morgan-Kiss, Rachael |
author_sort |
Kalra, Isha |
title |
Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity |
title_short |
Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity |
title_full |
Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity |
title_fullStr |
Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity |
title_full_unstemmed |
Chlamydomonas sp. UWO 241 Exhibits High Cyclic Electron Flow and Rewired Metabolism under High Salinity |
title_sort |
chlamydomonas sp. uwo 241 exhibits high cyclic electron flow and rewired metabolism under high salinity |
publishDate |
2022 |
url |
http://www.osti.gov/servlets/purl/1657213 https://www.osti.gov/biblio/1657213 https://doi.org/10.1104/pp.19.01280 |
long_lat |
ENVELOPE(-63.000,-63.000,-64.300,-64.300) ENVELOPE(165.100,165.100,-71.283,-71.283) |
geographic |
Antarctic The Antarctic Psi Calvin |
geographic_facet |
Antarctic The Antarctic Psi Calvin |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.osti.gov/servlets/purl/1657213 https://www.osti.gov/biblio/1657213 https://doi.org/10.1104/pp.19.01280 doi:10.1104/pp.19.01280 |
op_doi |
https://doi.org/10.1104/pp.19.01280 |
container_title |
Plant Physiology |
container_volume |
183 |
container_issue |
2 |
container_start_page |
588 |
op_container_end_page |
601 |
_version_ |
1772809563322449920 |