Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. ant...
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Online Access: | http://www.osti.gov/servlets/purl/1623920 https://www.osti.gov/biblio/1623920 https://doi.org/10.1038/ncomms3156 |
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ftosti:oai:osti.gov:1623920 2023-07-30T03:58:05+02:00 Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P. Reva, Oleg N. Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F. Yakimov, Michail M. Golyshina, Olga V. Reinhardt, Richard Golyshin, Peter N. 2023-07-03 application/pdf http://www.osti.gov/servlets/purl/1623920 https://www.osti.gov/biblio/1623920 https://doi.org/10.1038/ncomms3156 unknown http://www.osti.gov/servlets/purl/1623920 https://www.osti.gov/biblio/1623920 https://doi.org/10.1038/ncomms3156 doi:10.1038/ncomms3156 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1038/ncomms3156 2023-07-11T09:42:11Z Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. Other/Unknown Material Antarc* Antarctica SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Nature Communications 4 1 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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ftosti |
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unknown |
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59 BASIC BIOLOGICAL SCIENCES |
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59 BASIC BIOLOGICAL SCIENCES Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P. Reva, Oleg N. Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F. Yakimov, Michail M. Golyshina, Olga V. Reinhardt, Richard Golyshin, Peter N. Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Ubiquitous bacteria from the genus Oleispira drive oil degradation in the largest environment on Earth, the cold and deep sea. Here we report the genome sequence of Oleispira antarctica and show that compared with Alcanivorax borkumensis—the paradigm of mesophilic hydrocarbonoclastic bacteria—O. antarctica has a larger genome that has witnessed massive gene-transfer events. We identify an array of alkane monooxygenases, osmoprotectants, siderophores and micronutrient-scavenging pathways. We also show that at low temperatures, the main protein-folding machine Cpn60 functions as a single heptameric barrel that uses larger proteins as substrates compared with the classical double-barrel structure observed at higher temperatures. With 11 protein crystal structures, we further report the largest set of structures from one psychrotolerant organism. The most common structural feature is an increased content of surface-exposed negatively charged residues compared to their mesophilic counterparts. Our findings are relevant in the context of microbial cold-adaptation mechanisms and the development of strategies for oil-spill mitigation in cold environments. |
author |
Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P. Reva, Oleg N. Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F. Yakimov, Michail M. Golyshina, Olga V. Reinhardt, Richard Golyshin, Peter N. |
author_facet |
Kube, Michael Chernikova, Tatyana N. Al-Ramahi, Yamal Beloqui, Ana Lopez-Cortez, Nieves Guazzaroni, María-Eugenia Heipieper, Hermann J. Klages, Sven Kotsyurbenko, Oleg R. Langer, Ines Nechitaylo, Taras Y. Lünsdorf, Heinrich Fernández, Marisol Juárez, Silvia Ciordia, Sergio Singer, Alexander Kagan, Olga Egorova, Olga Alain Petit, Pierre Stogios, Peter Kim, Youngchang Tchigvintsev, Anatoli Flick, Robert Denaro, Renata Genovese, Maria Albar, Juan P. Reva, Oleg N. Martínez-Gomariz, Montserrat Tran, Hai Ferrer, Manuel Savchenko, Alexei Yakunin, Alexander F. Yakimov, Michail M. Golyshina, Olga V. Reinhardt, Richard Golyshin, Peter N. |
author_sort |
Kube, Michael |
title |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_short |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_full |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_fullStr |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_full_unstemmed |
Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica |
title_sort |
genome sequence and functional genomic analysis of the oil-degrading bacterium oleispira antarctica |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1623920 https://www.osti.gov/biblio/1623920 https://doi.org/10.1038/ncomms3156 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.osti.gov/servlets/purl/1623920 https://www.osti.gov/biblio/1623920 https://doi.org/10.1038/ncomms3156 doi:10.1038/ncomms3156 |
op_doi |
https://doi.org/10.1038/ncomms3156 |
container_title |
Nature Communications |
container_volume |
4 |
container_issue |
1 |
_version_ |
1772820993765539840 |