Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures
Climate change is causing arctic regions to warm disproportionally faster than those at lower latitudes, leading to alterations in carbon and nitrogen cycling, and potentially higher greenhouse gas emissions. It is thus increasingly important to better characterize the microorganisms driving arctic...
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ftosti:oai:osti.gov:1571953 2023-07-30T04:00:43+02:00 Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures Alves, Ricardo J. Eloy Kerou, Melina Zappe, Anna Bittner, Romana Abby, Sophie S. Schmidt, Heiko A. Pfeifer, Kevin Schleper, Christa 2023-06-30 application/pdf http://www.osti.gov/servlets/purl/1571953 https://www.osti.gov/biblio/1571953 https://doi.org/10.3389/fmicb.2019.01571 unknown http://www.osti.gov/servlets/purl/1571953 https://www.osti.gov/biblio/1571953 https://doi.org/10.3389/fmicb.2019.01571 doi:10.3389/fmicb.2019.01571 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.3389/fmicb.2019.01571 2023-07-11T09:37:49Z Climate change is causing arctic regions to warm disproportionally faster than those at lower latitudes, leading to alterations in carbon and nitrogen cycling, and potentially higher greenhouse gas emissions. It is thus increasingly important to better characterize the microorganisms driving arctic biogeochemical processes and their potential responses to changing conditions. Here, we describe a novel thaumarchaeon enriched from an arctic soil, Candidatus Nitrosocosmicus arcticus strain Kfb, which has been maintained for seven years in stable laboratory enrichment cultures as an aerobic ammonia oxidizer, with ammonium or urea as substrates. Genomic analyses show that this organism harbors all genes involved in ammonia oxidation and in carbon fixation via the 3-hydroxypropionate/4-hydroxybutyrate cycle, characteristic of all AOA, as well as the capability for urea utilization and potentially also for heterotrophic metabolism, similar to other AOA. Ca. N. arcticus oxidizes ammonia optimally between 20 and 28°C, well above average temperatures in its native high arctic environment (-13-4°C). Ammonia oxidation rates were nevertheless much lower than those of most cultivated mesophilic AOA (20-45°C). Intriguingly, we repeatedly observed apparent faster growth rates (based on marker gene counts) at lower temperatures (4-8°C) but without detectable nitrite production. Together with potential metabolisms predicted from its genome content, these observations indicate that Ca. N. arcticus is not a strict chemolithotrophic ammonia oxidizer and add to cumulating evidence for a greater metabolic and physiological versatility of AOA. The physiology of Ca. N. arcticus suggests that increasing temperatures might drastically affect nitrification in arctic soils by stimulating archaeal ammonia oxidation. Other/Unknown Material Arctic Climate change SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic Frontiers in Microbiology 10 |
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Open Polar |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
topic |
59 BASIC BIOLOGICAL SCIENCES |
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59 BASIC BIOLOGICAL SCIENCES Alves, Ricardo J. Eloy Kerou, Melina Zappe, Anna Bittner, Romana Abby, Sophie S. Schmidt, Heiko A. Pfeifer, Kevin Schleper, Christa Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Climate change is causing arctic regions to warm disproportionally faster than those at lower latitudes, leading to alterations in carbon and nitrogen cycling, and potentially higher greenhouse gas emissions. It is thus increasingly important to better characterize the microorganisms driving arctic biogeochemical processes and their potential responses to changing conditions. Here, we describe a novel thaumarchaeon enriched from an arctic soil, Candidatus Nitrosocosmicus arcticus strain Kfb, which has been maintained for seven years in stable laboratory enrichment cultures as an aerobic ammonia oxidizer, with ammonium or urea as substrates. Genomic analyses show that this organism harbors all genes involved in ammonia oxidation and in carbon fixation via the 3-hydroxypropionate/4-hydroxybutyrate cycle, characteristic of all AOA, as well as the capability for urea utilization and potentially also for heterotrophic metabolism, similar to other AOA. Ca. N. arcticus oxidizes ammonia optimally between 20 and 28°C, well above average temperatures in its native high arctic environment (-13-4°C). Ammonia oxidation rates were nevertheless much lower than those of most cultivated mesophilic AOA (20-45°C). Intriguingly, we repeatedly observed apparent faster growth rates (based on marker gene counts) at lower temperatures (4-8°C) but without detectable nitrite production. Together with potential metabolisms predicted from its genome content, these observations indicate that Ca. N. arcticus is not a strict chemolithotrophic ammonia oxidizer and add to cumulating evidence for a greater metabolic and physiological versatility of AOA. The physiology of Ca. N. arcticus suggests that increasing temperatures might drastically affect nitrification in arctic soils by stimulating archaeal ammonia oxidation. |
author |
Alves, Ricardo J. Eloy Kerou, Melina Zappe, Anna Bittner, Romana Abby, Sophie S. Schmidt, Heiko A. Pfeifer, Kevin Schleper, Christa |
author_facet |
Alves, Ricardo J. Eloy Kerou, Melina Zappe, Anna Bittner, Romana Abby, Sophie S. Schmidt, Heiko A. Pfeifer, Kevin Schleper, Christa |
author_sort |
Alves, Ricardo J. Eloy |
title |
Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures |
title_short |
Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures |
title_full |
Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures |
title_fullStr |
Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures |
title_full_unstemmed |
Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures |
title_sort |
ammonia oxidation by the arctic terrestrial thaumarchaeote candidatus nitrosocosmicus arcticus is stimulated by increasing temperatures |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1571953 https://www.osti.gov/biblio/1571953 https://doi.org/10.3389/fmicb.2019.01571 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change |
genre_facet |
Arctic Climate change |
op_relation |
http://www.osti.gov/servlets/purl/1571953 https://www.osti.gov/biblio/1571953 https://doi.org/10.3389/fmicb.2019.01571 doi:10.3389/fmicb.2019.01571 |
op_doi |
https://doi.org/10.3389/fmicb.2019.01571 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1772811272456241152 |