Metagenomic insights into diazotrophic communities across Arctic glacier forefields
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In to...
Published in: | FEMS Microbiology Ecology |
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Online Access: | http://www.osti.gov/servlets/purl/1543956 https://www.osti.gov/biblio/1543956 https://doi.org/10.1093/femsec/fiy114 |
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ftosti:oai:osti.gov:1543956 2023-07-30T04:01:27+02:00 Metagenomic insights into diazotrophic communities across Arctic glacier forefields Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia 2023-06-29 application/pdf http://www.osti.gov/servlets/purl/1543956 https://www.osti.gov/biblio/1543956 https://doi.org/10.1093/femsec/fiy114 unknown http://www.osti.gov/servlets/purl/1543956 https://www.osti.gov/biblio/1543956 https://doi.org/10.1093/femsec/fiy114 doi:10.1093/femsec/fiy114 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1093/femsec/fiy114 2023-07-11T09:35:11Z Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia . Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. Other/Unknown Material Arctic SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic FEMS Microbiology Ecology 94 9 |
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Open Polar |
collection |
SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
topic |
59 BASIC BIOLOGICAL SCIENCES |
spellingShingle |
59 BASIC BIOLOGICAL SCIENCES Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia . Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. |
author |
Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia |
author_facet |
Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. Sánchez-Baracaldo, Patricia |
author_sort |
Nash, Maisie V. |
title |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_short |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_fullStr |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full_unstemmed |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_sort |
metagenomic insights into diazotrophic communities across arctic glacier forefields |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1543956 https://www.osti.gov/biblio/1543956 https://doi.org/10.1093/femsec/fiy114 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://www.osti.gov/servlets/purl/1543956 https://www.osti.gov/biblio/1543956 https://doi.org/10.1093/femsec/fiy114 doi:10.1093/femsec/fiy114 |
op_doi |
https://doi.org/10.1093/femsec/fiy114 |
container_title |
FEMS Microbiology Ecology |
container_volume |
94 |
container_issue |
9 |
_version_ |
1772812183515693056 |