Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers

Nine microsatellite loci isolated in saffron cod ( Eleginus gracilis ) have potential applications for population genetics. Polymerase chain reaction products of samples of E. gracilis from northwestern Alaska amplified reliably, produced only one or two microsatellite bands, and had no apparent hom...

Full description

Bibliographic Details
Published in:Fishery Bulletin
Main Authors: Sme, Noel, Lyon, Sarah, Canino, Michael, Chernova, Natalia, O’Bryhim, Jason, Lance, Stacey, Jones, Kenneth, Mueter, Franz, Gharrett, Anthony
Language:unknown
Published: 2021
Subjects:
Online Access:http://www.osti.gov/servlets/purl/1512504
https://www.osti.gov/biblio/1512504
https://doi.org/10.7755/fb.116.1.6
id ftosti:oai:osti.gov:1512504
record_format openpolar
spelling ftosti:oai:osti.gov:1512504 2023-07-30T04:00:14+02:00 Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers Sme, Noel Lyon, Sarah Canino, Michael Chernova, Natalia O’Bryhim, Jason Lance, Stacey Jones, Kenneth Mueter, Franz Gharrett, Anthony 2021-07-30 application/pdf http://www.osti.gov/servlets/purl/1512504 https://www.osti.gov/biblio/1512504 https://doi.org/10.7755/fb.116.1.6 unknown http://www.osti.gov/servlets/purl/1512504 https://www.osti.gov/biblio/1512504 https://doi.org/10.7755/fb.116.1.6 doi:10.7755/fb.116.1.6 59 BASIC BIOLOGICAL SCIENCES 2021 ftosti https://doi.org/10.7755/fb.116.1.6 2023-07-11T09:33:15Z Nine microsatellite loci isolated in saffron cod ( Eleginus gracilis ) have potential applications for population genetics. Polymerase chain reaction products of samples of E. gracilis from northwestern Alaska amplified reliably, produced only one or two microsatellite bands, and had no apparent homozygote excess. A collection of E. gracilis sampled in the Gulf of Alaska (GOA) near Kodiak Island did not amplify reliably at one locus, and allele frequency profiles clustered distinctly (with principal component analysis [PCA]) from the northwestern Alaska collection. Northwestern Alaska and GOA E. gracilis collections were genetically different (on the basis of a standardized genetic differentiation measure [ G ' ST ]=0.313, chord distance [ D chord ]=0.078, P<0.0001) and differed in expected aver-age heterozygosities at shared loci (0.859 and 0.689, respectively). We tested the microsatellite primers on other gadid species endemic to the northern Pacific Ocean, Bering Sea, and Arctic Ocean for cross-species amplification. Not all loci amplified reliably in navaga ( E. nawaga ), Pacific tomcod ( Microgadus proximus ), Arctic cod ( Boreogadus saida ), Pacific cod ( Gadus macrocephalus ), or walleye pollock ( G. chalcogrammus ). Reliable loci varied in microsatellite size profiles and produced distinct PCA clusters and accurate genotype assignments that allowed accurate species identification. Furthermore the identifications support previous morphological and genetically determined systematic classifications and distinguished the geographically separated collections of E. gracilis . Other/Unknown Material Arctic cod Arctic Arctic Ocean Bering Sea Boreogadus saida Kodiak Alaska SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic Arctic Ocean Bering Sea Gulf of Alaska Pacific Navaga ENVELOPE(162.106,162.106,59.229,59.229) Fishery Bulletin 116 1 60 68
institution Open Polar
collection SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy)
op_collection_id ftosti
language unknown
topic 59 BASIC BIOLOGICAL SCIENCES
spellingShingle 59 BASIC BIOLOGICAL SCIENCES
Sme, Noel
Lyon, Sarah
Canino, Michael
Chernova, Natalia
O’Bryhim, Jason
Lance, Stacey
Jones, Kenneth
Mueter, Franz
Gharrett, Anthony
Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers
topic_facet 59 BASIC BIOLOGICAL SCIENCES
description Nine microsatellite loci isolated in saffron cod ( Eleginus gracilis ) have potential applications for population genetics. Polymerase chain reaction products of samples of E. gracilis from northwestern Alaska amplified reliably, produced only one or two microsatellite bands, and had no apparent homozygote excess. A collection of E. gracilis sampled in the Gulf of Alaska (GOA) near Kodiak Island did not amplify reliably at one locus, and allele frequency profiles clustered distinctly (with principal component analysis [PCA]) from the northwestern Alaska collection. Northwestern Alaska and GOA E. gracilis collections were genetically different (on the basis of a standardized genetic differentiation measure [ G ' ST ]=0.313, chord distance [ D chord ]=0.078, P<0.0001) and differed in expected aver-age heterozygosities at shared loci (0.859 and 0.689, respectively). We tested the microsatellite primers on other gadid species endemic to the northern Pacific Ocean, Bering Sea, and Arctic Ocean for cross-species amplification. Not all loci amplified reliably in navaga ( E. nawaga ), Pacific tomcod ( Microgadus proximus ), Arctic cod ( Boreogadus saida ), Pacific cod ( Gadus macrocephalus ), or walleye pollock ( G. chalcogrammus ). Reliable loci varied in microsatellite size profiles and produced distinct PCA clusters and accurate genotype assignments that allowed accurate species identification. Furthermore the identifications support previous morphological and genetically determined systematic classifications and distinguished the geographically separated collections of E. gracilis .
author Sme, Noel
Lyon, Sarah
Canino, Michael
Chernova, Natalia
O’Bryhim, Jason
Lance, Stacey
Jones, Kenneth
Mueter, Franz
Gharrett, Anthony
author_facet Sme, Noel
Lyon, Sarah
Canino, Michael
Chernova, Natalia
O’Bryhim, Jason
Lance, Stacey
Jones, Kenneth
Mueter, Franz
Gharrett, Anthony
author_sort Sme, Noel
title Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers
title_short Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers
title_full Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers
title_fullStr Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers
title_full_unstemmed Distinction of saffron cod ( Eleginus gracilis ) from several other gadid species by using microsatellite markers
title_sort distinction of saffron cod ( eleginus gracilis ) from several other gadid species by using microsatellite markers
publishDate 2021
url http://www.osti.gov/servlets/purl/1512504
https://www.osti.gov/biblio/1512504
https://doi.org/10.7755/fb.116.1.6
long_lat ENVELOPE(162.106,162.106,59.229,59.229)
geographic Arctic
Arctic Ocean
Bering Sea
Gulf of Alaska
Pacific
Navaga
geographic_facet Arctic
Arctic Ocean
Bering Sea
Gulf of Alaska
Pacific
Navaga
genre Arctic cod
Arctic
Arctic Ocean
Bering Sea
Boreogadus saida
Kodiak
Alaska
genre_facet Arctic cod
Arctic
Arctic Ocean
Bering Sea
Boreogadus saida
Kodiak
Alaska
op_relation http://www.osti.gov/servlets/purl/1512504
https://www.osti.gov/biblio/1512504
https://doi.org/10.7755/fb.116.1.6
doi:10.7755/fb.116.1.6
op_doi https://doi.org/10.7755/fb.116.1.6
container_title Fishery Bulletin
container_volume 116
container_issue 1
container_start_page 60
op_container_end_page 68
_version_ 1772810768273637376