The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill
The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spi...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , , , , , |
Language: | unknown |
Published: |
2023
|
Subjects: | |
Online Access: | http://www.osti.gov/servlets/purl/1479399 https://www.osti.gov/biblio/1479399 https://doi.org/10.3389/fmicb.2018.00808 |
id |
ftosti:oai:osti.gov:1479399 |
---|---|
record_format |
openpolar |
spelling |
ftosti:oai:osti.gov:1479399 2023-07-30T03:58:23+02:00 The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill Marietou, Angeliki Chastain, Roger Beulig, Felix Scoma, Alberto Hazen, Terry C. Bartlett, Douglas H. 2023-06-28 application/pdf http://www.osti.gov/servlets/purl/1479399 https://www.osti.gov/biblio/1479399 https://doi.org/10.3389/fmicb.2018.00808 unknown http://www.osti.gov/servlets/purl/1479399 https://www.osti.gov/biblio/1479399 https://doi.org/10.3389/fmicb.2018.00808 doi:10.3389/fmicb.2018.00808 2023 ftosti https://doi.org/10.3389/fmicb.2018.00808 2023-07-11T09:29:46Z The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spill at the Gulf of Mexico, we set up Macondo crude oil enrichments at wellhead temperature and different pressures to determine the effect of increasing depth/pressure to the in situ microbial community and their ability to degrade oil. We observed oil degradation under all pressure conditions tested [0.1, 15, and 30 megapascals (MPa)], although oil degradation profiles, cell numbers, and hydrocarbon degradation gene abundances indicated greatest activity at atmospheric pressure. Under all incubations the growth of psychrophilic bacteria was promoted. Bacteria closely related to Oleispira antarctica RB-8 dominated the communities at all pressures. At 30 MPa we observed a shift toward Photobacterium, a genus that includes piezophiles. Alphaproteobacterial members of the Sulfitobacter, previously associated with oil-degradation, were also highly abundant at 0.1 MPa. Our results suggest that pressure acts synergistically with low temperature to slow microbial growth and thus oil degradation in deep-sea environments. Other/Unknown Material Antarc* Antarctica SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Frontiers in Microbiology 9 |
institution |
Open Polar |
collection |
SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
description |
The Deepwater Horizon oil spill was one of the largest and deepest oil spills recorded. The wellhead was located at approximately 1500 m below the sea where low temperature and high pressure are key environmental characteristics. Using cells collected 4 months following the Deepwater Horizon oil spill at the Gulf of Mexico, we set up Macondo crude oil enrichments at wellhead temperature and different pressures to determine the effect of increasing depth/pressure to the in situ microbial community and their ability to degrade oil. We observed oil degradation under all pressure conditions tested [0.1, 15, and 30 megapascals (MPa)], although oil degradation profiles, cell numbers, and hydrocarbon degradation gene abundances indicated greatest activity at atmospheric pressure. Under all incubations the growth of psychrophilic bacteria was promoted. Bacteria closely related to Oleispira antarctica RB-8 dominated the communities at all pressures. At 30 MPa we observed a shift toward Photobacterium, a genus that includes piezophiles. Alphaproteobacterial members of the Sulfitobacter, previously associated with oil-degradation, were also highly abundant at 0.1 MPa. Our results suggest that pressure acts synergistically with low temperature to slow microbial growth and thus oil degradation in deep-sea environments. |
author |
Marietou, Angeliki Chastain, Roger Beulig, Felix Scoma, Alberto Hazen, Terry C. Bartlett, Douglas H. |
spellingShingle |
Marietou, Angeliki Chastain, Roger Beulig, Felix Scoma, Alberto Hazen, Terry C. Bartlett, Douglas H. The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
author_facet |
Marietou, Angeliki Chastain, Roger Beulig, Felix Scoma, Alberto Hazen, Terry C. Bartlett, Douglas H. |
author_sort |
Marietou, Angeliki |
title |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_short |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_full |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_fullStr |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_full_unstemmed |
The Effect of Hydrostatic Pressure on Enrichments of Hydrocarbon Degrading Microbes From the Gulf of Mexico Following the Deepwater Horizon Oil Spill |
title_sort |
effect of hydrostatic pressure on enrichments of hydrocarbon degrading microbes from the gulf of mexico following the deepwater horizon oil spill |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1479399 https://www.osti.gov/biblio/1479399 https://doi.org/10.3389/fmicb.2018.00808 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.osti.gov/servlets/purl/1479399 https://www.osti.gov/biblio/1479399 https://doi.org/10.3389/fmicb.2018.00808 doi:10.3389/fmicb.2018.00808 |
op_doi |
https://doi.org/10.3389/fmicb.2018.00808 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
_version_ |
1772821198772633600 |