Bacterial endophyte communities in the foliage of coast redwood and giant sequoia
The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria...
Published in: | Frontiers in Microbiology |
---|---|
Main Authors: | , |
Language: | unknown |
Published: |
2023
|
Subjects: | |
Online Access: | http://www.osti.gov/servlets/purl/1341541 https://www.osti.gov/biblio/1341541 https://doi.org/10.3389/fmicb.2015.01008 |
id |
ftosti:oai:osti.gov:1341541 |
---|---|
record_format |
openpolar |
spelling |
ftosti:oai:osti.gov:1341541 2023-07-30T04:01:40+02:00 Bacterial endophyte communities in the foliage of coast redwood and giant sequoia Carrell, Alyssa A. Frank, Anna C. 2023-06-26 application/pdf http://www.osti.gov/servlets/purl/1341541 https://www.osti.gov/biblio/1341541 https://doi.org/10.3389/fmicb.2015.01008 unknown http://www.osti.gov/servlets/purl/1341541 https://www.osti.gov/biblio/1341541 https://doi.org/10.3389/fmicb.2015.01008 doi:10.3389/fmicb.2015.01008 59 BASIC BIOLOGICAL SCIENCES 54 ENVIRONMENTAL SCIENCES 2023 ftosti https://doi.org/10.3389/fmicb.2015.01008 2023-07-11T09:17:00Z The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria (AAB) occurring at high relative abundance inside their needles. Our objective here was to determine if that pattern may be general to conifers, or alternatively, is more likely restricted to pines or conifers growing in nutrient limited and exposed environments. We used 16S rRNA pyrosequencing to characterize the foliar endophyte communities of two conifers in the Cupressaceae family: Two coast redwood (CR; Sequoia sempervirens) populations and one giant sequoia (GS; Sequoiadendron giganteum) population were sampled. Similar to the pines, the endophyte communities of the giant trees were dominated by Proteobacteria, Firmicutes, Acidobacteria, and Actinobacteria. However, although some major operational taxonomic units (OTUs) occurred at a high relative abundance of 10–40% in multiple samples, no specific group of bacteria dominated the endophyte community to the extent previously observed in high-elevation pines. Several of the dominating bacterial groups in the CR and GS foliage (e.g., Bacillus, Burkholderia, Actinomycetes) are known for disease- and pest suppression, raising the possibility that the endophytic microbiome protects the giant trees against biotic stress. Many of the most common and abundant OTUs in our dataset were most similar to 16S rRNA sequences from bacteria found in lichens or arctic plants. For example, an OTU belonging to the uncultured Rhizobiales LAR1 lineage, which is commonly associated with lichens, was observed at high relative abundance in many of the CR samples. Lastly, the taxa shared between the giant trees, arctic plants, and lichens may be part of a broadly defined endophyte microbiome common to temperate, boreal, and tundra ecosystems. Other/Unknown Material Arctic Tundra SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic Frontiers in Microbiology 6 |
institution |
Open Polar |
collection |
SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
op_collection_id |
ftosti |
language |
unknown |
topic |
59 BASIC BIOLOGICAL SCIENCES 54 ENVIRONMENTAL SCIENCES |
spellingShingle |
59 BASIC BIOLOGICAL SCIENCES 54 ENVIRONMENTAL SCIENCES Carrell, Alyssa A. Frank, Anna C. Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES 54 ENVIRONMENTAL SCIENCES |
description |
The endophytic bacterial microbiome, with an emerging role in plant nutrient acquisition and stress tolerance, is much less studied in natural plant populations than in agricultural crops. In a previous study, we found consistent associations between trees in the pine family and acetic acid bacteria (AAB) occurring at high relative abundance inside their needles. Our objective here was to determine if that pattern may be general to conifers, or alternatively, is more likely restricted to pines or conifers growing in nutrient limited and exposed environments. We used 16S rRNA pyrosequencing to characterize the foliar endophyte communities of two conifers in the Cupressaceae family: Two coast redwood (CR; Sequoia sempervirens) populations and one giant sequoia (GS; Sequoiadendron giganteum) population were sampled. Similar to the pines, the endophyte communities of the giant trees were dominated by Proteobacteria, Firmicutes, Acidobacteria, and Actinobacteria. However, although some major operational taxonomic units (OTUs) occurred at a high relative abundance of 10–40% in multiple samples, no specific group of bacteria dominated the endophyte community to the extent previously observed in high-elevation pines. Several of the dominating bacterial groups in the CR and GS foliage (e.g., Bacillus, Burkholderia, Actinomycetes) are known for disease- and pest suppression, raising the possibility that the endophytic microbiome protects the giant trees against biotic stress. Many of the most common and abundant OTUs in our dataset were most similar to 16S rRNA sequences from bacteria found in lichens or arctic plants. For example, an OTU belonging to the uncultured Rhizobiales LAR1 lineage, which is commonly associated with lichens, was observed at high relative abundance in many of the CR samples. Lastly, the taxa shared between the giant trees, arctic plants, and lichens may be part of a broadly defined endophyte microbiome common to temperate, boreal, and tundra ecosystems. |
author |
Carrell, Alyssa A. Frank, Anna C. |
author_facet |
Carrell, Alyssa A. Frank, Anna C. |
author_sort |
Carrell, Alyssa A. |
title |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_short |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_full |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_fullStr |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_full_unstemmed |
Bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
title_sort |
bacterial endophyte communities in the foliage of coast redwood and giant sequoia |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1341541 https://www.osti.gov/biblio/1341541 https://doi.org/10.3389/fmicb.2015.01008 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Tundra |
genre_facet |
Arctic Tundra |
op_relation |
http://www.osti.gov/servlets/purl/1341541 https://www.osti.gov/biblio/1341541 https://doi.org/10.3389/fmicb.2015.01008 doi:10.3389/fmicb.2015.01008 |
op_doi |
https://doi.org/10.3389/fmicb.2015.01008 |
container_title |
Frontiers in Microbiology |
container_volume |
6 |
_version_ |
1772812430090436608 |