High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert

In this paper we report the bacterial diversity of biological soil crusts (biocrusts) inhabiting polar desert soils at the northern land limit of the Arctic polar region (83° 05 N). Employing pyrosequencing of bacterial 16S rRNA genes this study demonstrated that these biocrusts harbor diverse bacte...

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Published in:PLoS ONE
Main Authors: Steven, Blaire, Lionard, Marie, Kuske, Cheryl R., Vincent, Warwick F.
Language:unknown
Published: 2023
Subjects:
Online Access:http://www.osti.gov/servlets/purl/1321727
https://www.osti.gov/biblio/1321727
https://doi.org/10.1371/journal.pone.0071489
id ftosti:oai:osti.gov:1321727
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spelling ftosti:oai:osti.gov:1321727 2023-07-30T04:01:22+02:00 High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert Steven, Blaire Lionard, Marie Kuske, Cheryl R. Vincent, Warwick F. 2023-06-26 application/pdf http://www.osti.gov/servlets/purl/1321727 https://www.osti.gov/biblio/1321727 https://doi.org/10.1371/journal.pone.0071489 unknown http://www.osti.gov/servlets/purl/1321727 https://www.osti.gov/biblio/1321727 https://doi.org/10.1371/journal.pone.0071489 doi:10.1371/journal.pone.0071489 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1371/journal.pone.0071489 2023-07-11T09:14:57Z In this paper we report the bacterial diversity of biological soil crusts (biocrusts) inhabiting polar desert soils at the northern land limit of the Arctic polar region (83° 05 N). Employing pyrosequencing of bacterial 16S rRNA genes this study demonstrated that these biocrusts harbor diverse bacterial communities, often as diverse as temperate latitude communities. The effect of wetting pulses on the composition of communities was also determined by collecting samples from soils outside and inside of permafrost water tracks, hill slope flow paths that drain permafrost-affected soils. The intermittent flow regime in the water tracks was correlated with altered relative abundance of phylum level taxonomic bins in the bacterial communities, but the alterations varied between individual sampling sites. Bacteria related to the Cyanobacteria and Acidobacteria demonstrated shifts in relative abundance based on their location either inside or outside of the water tracks. Among cyanobacterial sequences, the proportion of sequences belonging to the family Oscillatoriales consistently increased in relative abundance in the samples from inside the water tracks compared to those outside. Acidobacteria showed responses to wetting pulses in the water tracks, increasing in abundance at one site and decreasing at the other two sites. Subdivision 4 acidobacterial sequences tended to follow the trends in the total Acidobacteria relative abundance, suggesting these organisms were largely responsible for the changes observed in the Acidobacteria. Finally, taken together, these data suggest that the bacterial communities of these high latitude polar biocrusts are diverse but do not show a consensus response to intermittent flow in water tracks over high Arctic permafrost. Other/Unknown Material Arctic permafrost polar desert SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic PLoS ONE 8 8 e71489
institution Open Polar
collection SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy)
op_collection_id ftosti
language unknown
topic 59 BASIC BIOLOGICAL SCIENCES
spellingShingle 59 BASIC BIOLOGICAL SCIENCES
Steven, Blaire
Lionard, Marie
Kuske, Cheryl R.
Vincent, Warwick F.
High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert
topic_facet 59 BASIC BIOLOGICAL SCIENCES
description In this paper we report the bacterial diversity of biological soil crusts (biocrusts) inhabiting polar desert soils at the northern land limit of the Arctic polar region (83° 05 N). Employing pyrosequencing of bacterial 16S rRNA genes this study demonstrated that these biocrusts harbor diverse bacterial communities, often as diverse as temperate latitude communities. The effect of wetting pulses on the composition of communities was also determined by collecting samples from soils outside and inside of permafrost water tracks, hill slope flow paths that drain permafrost-affected soils. The intermittent flow regime in the water tracks was correlated with altered relative abundance of phylum level taxonomic bins in the bacterial communities, but the alterations varied between individual sampling sites. Bacteria related to the Cyanobacteria and Acidobacteria demonstrated shifts in relative abundance based on their location either inside or outside of the water tracks. Among cyanobacterial sequences, the proportion of sequences belonging to the family Oscillatoriales consistently increased in relative abundance in the samples from inside the water tracks compared to those outside. Acidobacteria showed responses to wetting pulses in the water tracks, increasing in abundance at one site and decreasing at the other two sites. Subdivision 4 acidobacterial sequences tended to follow the trends in the total Acidobacteria relative abundance, suggesting these organisms were largely responsible for the changes observed in the Acidobacteria. Finally, taken together, these data suggest that the bacterial communities of these high latitude polar biocrusts are diverse but do not show a consensus response to intermittent flow in water tracks over high Arctic permafrost.
author Steven, Blaire
Lionard, Marie
Kuske, Cheryl R.
Vincent, Warwick F.
author_facet Steven, Blaire
Lionard, Marie
Kuske, Cheryl R.
Vincent, Warwick F.
author_sort Steven, Blaire
title High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert
title_short High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert
title_full High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert
title_fullStr High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert
title_full_unstemmed High Bacterial Diversity of Biological Soil Crusts in Water Tracks over Permafrost in the High Arctic Polar Desert
title_sort high bacterial diversity of biological soil crusts in water tracks over permafrost in the high arctic polar desert
publishDate 2023
url http://www.osti.gov/servlets/purl/1321727
https://www.osti.gov/biblio/1321727
https://doi.org/10.1371/journal.pone.0071489
geographic Arctic
geographic_facet Arctic
genre Arctic
permafrost
polar desert
genre_facet Arctic
permafrost
polar desert
op_relation http://www.osti.gov/servlets/purl/1321727
https://www.osti.gov/biblio/1321727
https://doi.org/10.1371/journal.pone.0071489
doi:10.1371/journal.pone.0071489
op_doi https://doi.org/10.1371/journal.pone.0071489
container_title PLoS ONE
container_volume 8
container_issue 8
container_start_page e71489
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