Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla
The surface waters of oligotrophic oceans have chronically low phosphate (P i ) concentrations, which renders dissolved organic phosphorus (DOP) an important nutrient source. In the subtropical North Atlantic, cyanobacteria are often numerically dominant, but picoeukaryotes can dominate autotrophic...
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Online Access: | http://www.osti.gov/servlets/purl/1283057 https://www.osti.gov/biblio/1283057 https://doi.org/10.1371/journal.pone.0155158 |
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ftosti:oai:osti.gov:1283057 2023-07-30T04:05:31+02:00 Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla Whitney, LeAnn P. Lomas, Michael W. 2023-06-26 application/pdf http://www.osti.gov/servlets/purl/1283057 https://www.osti.gov/biblio/1283057 https://doi.org/10.1371/journal.pone.0155158 unknown http://www.osti.gov/servlets/purl/1283057 https://www.osti.gov/biblio/1283057 https://doi.org/10.1371/journal.pone.0155158 doi:10.1371/journal.pone.0155158 59 BASIC BIOLOGICAL SCIENCES 2023 ftosti https://doi.org/10.1371/journal.pone.0155158 2023-07-11T09:09:50Z The surface waters of oligotrophic oceans have chronically low phosphate (P i ) concentrations, which renders dissolved organic phosphorus (DOP) an important nutrient source. In the subtropical North Atlantic, cyanobacteria are often numerically dominant, but picoeukaryotes can dominate autotrophic biomass and productivity making them important contributors to the ocean carbon cycle. Despite their importance, little is known regarding the metabolic response of picoeukaryotes to changes in phosphorus (P) source and availability. To understand the molecular mechanisms that regulate P utilization in oligotrophic environments, we evaluated transcriptomes of the picoeukaryote Micromonas pusilla grown under P i -replete and -deficient conditions, with an additional investigation of growth on DOP in replete conditions. Genes that function in sulfolipid substitution and P i uptake increased in expression with P i -deficiency, suggesting cells were reallocating cellular P and increasing P acquisition capabilities. P i -deficient M. pusilla cells also increased alkaline phosphatase activity and reduced their cellular P content. Cells grown with DOP were able to maintain relatively high growth rates, however the transcriptomic response was more similar to the P i -deficient response than that seen in cells grown under P i -replete conditions. The results demonstrate that not all P sources are the same for growth; while M. pusilla , a model picoeukaryote, may grow well on DOP, the metabolic demand is greater than growth on P i . Lastly, these findings provide insight into the cellular strategies which may be used to support growth in a stratified future ocean predicted to favor picoeukaryotes. Other/Unknown Material North Atlantic SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) PLOS ONE 11 5 e0155158 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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ftosti |
language |
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59 BASIC BIOLOGICAL SCIENCES |
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59 BASIC BIOLOGICAL SCIENCES Whitney, LeAnn P. Lomas, Michael W. Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla |
topic_facet |
59 BASIC BIOLOGICAL SCIENCES |
description |
The surface waters of oligotrophic oceans have chronically low phosphate (P i ) concentrations, which renders dissolved organic phosphorus (DOP) an important nutrient source. In the subtropical North Atlantic, cyanobacteria are often numerically dominant, but picoeukaryotes can dominate autotrophic biomass and productivity making them important contributors to the ocean carbon cycle. Despite their importance, little is known regarding the metabolic response of picoeukaryotes to changes in phosphorus (P) source and availability. To understand the molecular mechanisms that regulate P utilization in oligotrophic environments, we evaluated transcriptomes of the picoeukaryote Micromonas pusilla grown under P i -replete and -deficient conditions, with an additional investigation of growth on DOP in replete conditions. Genes that function in sulfolipid substitution and P i uptake increased in expression with P i -deficiency, suggesting cells were reallocating cellular P and increasing P acquisition capabilities. P i -deficient M. pusilla cells also increased alkaline phosphatase activity and reduced their cellular P content. Cells grown with DOP were able to maintain relatively high growth rates, however the transcriptomic response was more similar to the P i -deficient response than that seen in cells grown under P i -replete conditions. The results demonstrate that not all P sources are the same for growth; while M. pusilla , a model picoeukaryote, may grow well on DOP, the metabolic demand is greater than growth on P i . Lastly, these findings provide insight into the cellular strategies which may be used to support growth in a stratified future ocean predicted to favor picoeukaryotes. |
author |
Whitney, LeAnn P. Lomas, Michael W. |
author_facet |
Whitney, LeAnn P. Lomas, Michael W. |
author_sort |
Whitney, LeAnn P. |
title |
Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla |
title_short |
Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla |
title_full |
Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla |
title_fullStr |
Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla |
title_full_unstemmed |
Growth on ATP elicits a P-stress response in the picoeukaryote Micromonas pusilla |
title_sort |
growth on atp elicits a p-stress response in the picoeukaryote micromonas pusilla |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1283057 https://www.osti.gov/biblio/1283057 https://doi.org/10.1371/journal.pone.0155158 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://www.osti.gov/servlets/purl/1283057 https://www.osti.gov/biblio/1283057 https://doi.org/10.1371/journal.pone.0155158 doi:10.1371/journal.pone.0155158 |
op_doi |
https://doi.org/10.1371/journal.pone.0155158 |
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PLOS ONE |
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11 |
container_issue |
5 |
container_start_page |
e0155158 |
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1772817482069835776 |