Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry
Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its...
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ftosti:oai:osti.gov:1212345 2023-07-30T04:01:54+02:00 Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry Mann, Benjamin F Chen, Hongmei Herndon, Elizabeth M Chu, Rosalie K. Tolic, Nikola Portier, Evan F Robinson, Errol W. Callister, Stephen J Wullschleger, Stan D Graham, David E Liang, Liyuan Gu, Baohua 2023-06-26 application/pdf http://www.osti.gov/servlets/purl/1212345 https://www.osti.gov/biblio/1212345 https://doi.org/10.1371/journal.pone.0130557 unknown http://www.osti.gov/servlets/purl/1212345 https://www.osti.gov/biblio/1212345 https://doi.org/10.1371/journal.pone.0130557 doi:10.1371/journal.pone.0130557 58 GEOSCIENCES 54 ENVIRONMENTAL SCIENCES 2023 ftosti https://doi.org/10.1371/journal.pone.0130557 2023-07-11T09:02:41Z Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its degradation during a simulated warming experiment. A soil sample, collected near Barrow, Alaska, USA, was subjected to a 40-day incubation under anoxic conditions and analyzed before and after the incubation to determine changes of SOM composition. A CHO index based on molecular C, H, and O data was utilized to codify SOM components according to their observed degradation potentials. Compounds with a CHO index score between 1 and 0 in a water-soluble fraction (WSF) demonstrated high degradation potential, with a highest shift of CHO index occurred in the N-containing group of compounds, while similar stoichiometries in a base-soluble fraction (BSF) did not. Additionally, compared with the classical H:C vs O:C van Krevelen diagram, CHO index allowed for direct visualization of the distribution of heteroatoms such as N in the identified SOM compounds. We demonstrate that CHO index is useful not only in characterizing arctic SOM at the molecular level but also enabling quantitative description of SOM degradation, thereby facilitating incorporation of the high resolution MS datasets to future mechanistic models of SOM degradation and prediction of greenhouse gas emissions. Other/Unknown Material Arctic Barrow permafrost Alaska SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic PLOS ONE 10 6 e0130557 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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ftosti |
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58 GEOSCIENCES 54 ENVIRONMENTAL SCIENCES |
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58 GEOSCIENCES 54 ENVIRONMENTAL SCIENCES Mann, Benjamin F Chen, Hongmei Herndon, Elizabeth M Chu, Rosalie K. Tolic, Nikola Portier, Evan F Robinson, Errol W. Callister, Stephen J Wullschleger, Stan D Graham, David E Liang, Liyuan Gu, Baohua Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
topic_facet |
58 GEOSCIENCES 54 ENVIRONMENTAL SCIENCES |
description |
Microbial degradation of soil organic matter (SOM) is a key process for terrestrial carbon cycling, although the molecular details of these transformations remain unclear. This study reports the application of ultrahigh resolution mass spectrometry to profile the molecular composition of SOM and its degradation during a simulated warming experiment. A soil sample, collected near Barrow, Alaska, USA, was subjected to a 40-day incubation under anoxic conditions and analyzed before and after the incubation to determine changes of SOM composition. A CHO index based on molecular C, H, and O data was utilized to codify SOM components according to their observed degradation potentials. Compounds with a CHO index score between 1 and 0 in a water-soluble fraction (WSF) demonstrated high degradation potential, with a highest shift of CHO index occurred in the N-containing group of compounds, while similar stoichiometries in a base-soluble fraction (BSF) did not. Additionally, compared with the classical H:C vs O:C van Krevelen diagram, CHO index allowed for direct visualization of the distribution of heteroatoms such as N in the identified SOM compounds. We demonstrate that CHO index is useful not only in characterizing arctic SOM at the molecular level but also enabling quantitative description of SOM degradation, thereby facilitating incorporation of the high resolution MS datasets to future mechanistic models of SOM degradation and prediction of greenhouse gas emissions. |
author |
Mann, Benjamin F Chen, Hongmei Herndon, Elizabeth M Chu, Rosalie K. Tolic, Nikola Portier, Evan F Robinson, Errol W. Callister, Stephen J Wullschleger, Stan D Graham, David E Liang, Liyuan Gu, Baohua |
author_facet |
Mann, Benjamin F Chen, Hongmei Herndon, Elizabeth M Chu, Rosalie K. Tolic, Nikola Portier, Evan F Robinson, Errol W. Callister, Stephen J Wullschleger, Stan D Graham, David E Liang, Liyuan Gu, Baohua |
author_sort |
Mann, Benjamin F |
title |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_short |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_full |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_fullStr |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_full_unstemmed |
Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry |
title_sort |
indexing permafrost soil organic matter degradation using high-resolution mass spectrometry |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1212345 https://www.osti.gov/biblio/1212345 https://doi.org/10.1371/journal.pone.0130557 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Barrow permafrost Alaska |
genre_facet |
Arctic Barrow permafrost Alaska |
op_relation |
http://www.osti.gov/servlets/purl/1212345 https://www.osti.gov/biblio/1212345 https://doi.org/10.1371/journal.pone.0130557 doi:10.1371/journal.pone.0130557 |
op_doi |
https://doi.org/10.1371/journal.pone.0130557 |
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PLOS ONE |
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10 |
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6 |
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e0130557 |
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