Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses
The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N)...
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ftosti:oai:osti.gov:1204352 2023-07-30T04:01:34+02:00 Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Taş, Neslihan Elberling, Bo Jansson, Janet K. Semenchuk, Philipp Priemé, Anders 2023-06-26 application/pdf http://www.osti.gov/servlets/purl/1204352 https://www.osti.gov/biblio/1204352 https://doi.org/10.3389/fmicb.2015.00399 unknown http://www.osti.gov/servlets/purl/1204352 https://www.osti.gov/biblio/1204352 https://doi.org/10.3389/fmicb.2015.00399 doi:10.3389/fmicb.2015.00399 58 GEOSCIENCES 2023 ftosti https://doi.org/10.3389/fmicb.2015.00399 2023-07-11T09:01:48Z The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below -10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface. Other/Unknown Material Arctic permafrost Svalbard SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) Arctic Svalbard Frontiers in Microbiology 6 |
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SciTec Connect (Office of Scientific and Technical Information - OSTI, U.S. Department of Energy) |
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58 GEOSCIENCES |
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58 GEOSCIENCES Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Taş, Neslihan Elberling, Bo Jansson, Janet K. Semenchuk, Philipp Priemé, Anders Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
topic_facet |
58 GEOSCIENCES |
description |
The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below -10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface. |
author |
Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Taş, Neslihan Elberling, Bo Jansson, Janet K. Semenchuk, Philipp Priemé, Anders |
author_facet |
Schostag, Morten Stibal, Marek Jacobsen, Carsten S. Bælum, Jacob Taş, Neslihan Elberling, Bo Jansson, Janet K. Semenchuk, Philipp Priemé, Anders |
author_sort |
Schostag, Morten |
title |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_short |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_full |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_fullStr |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_full_unstemmed |
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses |
title_sort |
distinct summer and winter bacterial communities in the active layer of svalbard permafrost revealed by dna- and rna-based analyses |
publishDate |
2023 |
url |
http://www.osti.gov/servlets/purl/1204352 https://www.osti.gov/biblio/1204352 https://doi.org/10.3389/fmicb.2015.00399 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic permafrost Svalbard |
genre_facet |
Arctic permafrost Svalbard |
op_relation |
http://www.osti.gov/servlets/purl/1204352 https://www.osti.gov/biblio/1204352 https://doi.org/10.3389/fmicb.2015.00399 doi:10.3389/fmicb.2015.00399 |
op_doi |
https://doi.org/10.3389/fmicb.2015.00399 |
container_title |
Frontiers in Microbiology |
container_volume |
6 |
_version_ |
1772812336958013440 |