What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance?
Little is known about the evolution of cold tolerance in polar plant species and how they differ from temperate relatives. To gain insight into their biology and the evolution of cold tolerance, we compared the molecular basis of cold response in three Arctic Brassicaceae species. We conducted a com...
Published in: | Molecular Ecology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | http://hdl.handle.net/10852/99671 https://doi.org/10.1111/mec.16581 |
id |
ftoslouniv:oai:www.duo.uio.no:10852/99671 |
---|---|
record_format |
openpolar |
spelling |
ftoslouniv:oai:www.duo.uio.no:10852/99671 2023-05-15T14:27:31+02:00 What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? ENEngelskEnglishWhat can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? Birkeland, Siri Slotte, Tanja Brysting, Anne Krag Gustafsson, Lovisa Hvidsten, Torgeir Rhoden Brochmann, Christian Nowak, Michael Dennis 2022-09-19T15:55:37Z http://hdl.handle.net/10852/99671 https://doi.org/10.1111/mec.16581 EN eng NFR/240223 NFR/274607 NFR/287465 Birkeland, Siri Slotte, Tanja Brysting, Anne Krag Gustafsson, Lovisa Hvidsten, Torgeir Rhoden Brochmann, Christian Nowak, Michael Dennis . What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance?. Molecular Ecology. 2022, 31(16), 4271-4285 http://hdl.handle.net/10852/99671 2053236 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular Ecology&rft.volume=31&rft.spage=4271&rft.date=2022 Molecular Ecology 31 16 4271 4285 https://doi.org/10.1111/mec.16581 Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ CC-BY 0962-1083 Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2022 ftoslouniv https://doi.org/10.1111/mec.16581 2023-02-08T23:36:26Z Little is known about the evolution of cold tolerance in polar plant species and how they differ from temperate relatives. To gain insight into their biology and the evolution of cold tolerance, we compared the molecular basis of cold response in three Arctic Brassicaceae species. We conducted a comparative time series experiment to examine transcriptional responses to low temperature. RNA was sampled at 22°C, and after 3, 6, and 24 at 2°C. We then identified sets of genes that were differentially expressed in response to cold and compared them between species, as well as to published data from the temperate Arabidopsis thaliana. Most differentially expressed genes were species-specific, but a significant portion of the cold response was also shared among species. Among thousands of differentially expressed genes, ~200 were shared among the three Arctic species and A. thaliana, while ~100 were exclusively shared among the three Arctic species. Our results show that cold response differs markedly between Arctic Brassicaceae species, but probably builds on a conserved basis found across the family. They also confirm that highly polygenic traits such as cold tolerance may show little repeatability in their patterns of adaptation. Article in Journal/Newspaper Arctic Arctic Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Arctic Molecular Ecology 31 16 4271 4285 |
institution |
Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
Little is known about the evolution of cold tolerance in polar plant species and how they differ from temperate relatives. To gain insight into their biology and the evolution of cold tolerance, we compared the molecular basis of cold response in three Arctic Brassicaceae species. We conducted a comparative time series experiment to examine transcriptional responses to low temperature. RNA was sampled at 22°C, and after 3, 6, and 24 at 2°C. We then identified sets of genes that were differentially expressed in response to cold and compared them between species, as well as to published data from the temperate Arabidopsis thaliana. Most differentially expressed genes were species-specific, but a significant portion of the cold response was also shared among species. Among thousands of differentially expressed genes, ~200 were shared among the three Arctic species and A. thaliana, while ~100 were exclusively shared among the three Arctic species. Our results show that cold response differs markedly between Arctic Brassicaceae species, but probably builds on a conserved basis found across the family. They also confirm that highly polygenic traits such as cold tolerance may show little repeatability in their patterns of adaptation. |
format |
Article in Journal/Newspaper |
author |
Birkeland, Siri Slotte, Tanja Brysting, Anne Krag Gustafsson, Lovisa Hvidsten, Torgeir Rhoden Brochmann, Christian Nowak, Michael Dennis |
spellingShingle |
Birkeland, Siri Slotte, Tanja Brysting, Anne Krag Gustafsson, Lovisa Hvidsten, Torgeir Rhoden Brochmann, Christian Nowak, Michael Dennis What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? |
author_facet |
Birkeland, Siri Slotte, Tanja Brysting, Anne Krag Gustafsson, Lovisa Hvidsten, Torgeir Rhoden Brochmann, Christian Nowak, Michael Dennis |
author_sort |
Birkeland, Siri |
title |
What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? |
title_short |
What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? |
title_full |
What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? |
title_fullStr |
What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? |
title_full_unstemmed |
What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance? |
title_sort |
what can cold-induced transcriptomes of arctic brassicaceae tell us about the evolution of cold tolerance? |
publishDate |
2022 |
url |
http://hdl.handle.net/10852/99671 https://doi.org/10.1111/mec.16581 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_source |
0962-1083 |
op_relation |
NFR/240223 NFR/274607 NFR/287465 Birkeland, Siri Slotte, Tanja Brysting, Anne Krag Gustafsson, Lovisa Hvidsten, Torgeir Rhoden Brochmann, Christian Nowak, Michael Dennis . What can cold-induced transcriptomes of Arctic Brassicaceae tell us about the evolution of cold tolerance?. Molecular Ecology. 2022, 31(16), 4271-4285 http://hdl.handle.net/10852/99671 2053236 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular Ecology&rft.volume=31&rft.spage=4271&rft.date=2022 Molecular Ecology 31 16 4271 4285 https://doi.org/10.1111/mec.16581 |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/mec.16581 |
container_title |
Molecular Ecology |
container_volume |
31 |
container_issue |
16 |
container_start_page |
4271 |
op_container_end_page |
4285 |
_version_ |
1766301285179457536 |