Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet
Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation....
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2021
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ftoslouniv:oai:www.duo.uio.no:10852/86708 2023-05-15T16:13:45+02:00 Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve 2021-07-20T12:53:36Z http://hdl.handle.net/10852/86708 http://urn.nb.no/URN:NBN:no-89346 https://doi.org/10.1002/edn3.237 EN eng http://urn.nb.no/URN:NBN:no-89346 Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve . Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet. Environmental DNA. 2021 http://hdl.handle.net/10852/86708 1922232 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental DNA&rft.volume=&rft.spage=&rft.date=2021 Environmental DNA 16 https://doi.org/10.1002/edn3.237 URN:NBN:no-89346 Fulltext https://www.duo.uio.no/bitstream/handle/10852/86708/2/edn3.237.pdf Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ CC-BY 2637-4943 Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2021 ftoslouniv https://doi.org/10.1002/edn3.237 2021-08-11T22:31:36Z Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation. Non-molecular means used to study their diet are time-consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here, we use non-invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faces to present the first large-scale molecular dietary analysis of capercaillies. Facal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n = 172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus, respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F5,86 = 11.01, r2 = 0.17, p = 0.001) and seasons (adonis pseudo F2,03 = 0.64, r2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F1,47 = 2.77, r2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared with existing knowledge from non-molecular means. The non-invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies’ diet, improving our understanding of adequate habitat required for their conservation. Article in Journal/Newspaper Finnmark Finnmark Troms Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Jura ENVELOPE(13.501,13.501,68.062,68.062) Norway Environmental DNA |
institution |
Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
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ftoslouniv |
language |
English |
description |
Conservation strategies centered around species habitat protection rely on species’ dietary information. One species at the focal point of conservation efforts is the herbivorous grouse, the western capercaillie (Tetrao urogallus), which is an indicator species for forest biodiversity conservation. Non-molecular means used to study their diet are time-consuming and at low taxonomic resolution. This delays the implementation of conservation strategies including resource protection due to uncertainty about its diet. Thus, limited knowledge on diet is hampering conservation efforts. Here, we use non-invasive environmental DNA (eDNA) metabarcoding on DNA extracted from faces to present the first large-scale molecular dietary analysis of capercaillies. Facal samples were collected from seven populations located in Norway (Finnmark, Troms, Trøndelag, Innlandet) and France (Vosges, Jura, Pyrenees) (n = 172). We detected 122 plant taxa belonging to 46 plant families of which 37.7% of the detected taxa could be identified at species level. The average dietary richness of each sample was 7 ± 5 SD taxa. The most frequently occurring plant groups with the highest relative read abundance (RRA) were trees and dwarf shrubs, in particular, Pinus and Vaccinium myrtillus, respectively. There was a difference in dietary composition (RRA) between samples collected from the different locations (adonis pseudo F5,86 = 11.01, r2 = 0.17, p = 0.001) and seasons (adonis pseudo F2,03 = 0.64, r2 = 0.01, p = 0.036). Dietary composition also differed between sexes at each location (adonis pseudo F1,47 = 2.77, r2 = 0.04, p = 0.024), although not significant for all data combined. In total, 35 taxa (36.8% of taxa recorded) were new capercaillie food items compared with existing knowledge from non-molecular means. The non-invasive molecular dietary analysis applied in this study provides new ecological information of capercaillies’ diet, improving our understanding of adequate habitat required for their conservation. |
format |
Article in Journal/Newspaper |
author |
Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve |
spellingShingle |
Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
author_facet |
Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve |
author_sort |
Chua, Physilia Y. S. |
title |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_short |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_fullStr |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_full_unstemmed |
Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet |
title_sort |
molecular dietary analyses of western capercaillies (tetrao urogallus) reveal a diverse diet |
publishDate |
2021 |
url |
http://hdl.handle.net/10852/86708 http://urn.nb.no/URN:NBN:no-89346 https://doi.org/10.1002/edn3.237 |
long_lat |
ENVELOPE(13.501,13.501,68.062,68.062) |
geographic |
Jura Norway |
geographic_facet |
Jura Norway |
genre |
Finnmark Finnmark Troms |
genre_facet |
Finnmark Finnmark Troms |
op_source |
2637-4943 |
op_relation |
http://urn.nb.no/URN:NBN:no-89346 Chua, Physilia Y. S. Lammers, Youri Menoni, Emmanuel Ekrem, Torbjørn Bohmann, Kristine Boessenkool, Sanne Alsos, Inger Greve . Molecular dietary analyses of western capercaillies (Tetrao urogallus) reveal a diverse diet. Environmental DNA. 2021 http://hdl.handle.net/10852/86708 1922232 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental DNA&rft.volume=&rft.spage=&rft.date=2021 Environmental DNA 16 https://doi.org/10.1002/edn3.237 URN:NBN:no-89346 Fulltext https://www.duo.uio.no/bitstream/handle/10852/86708/2/edn3.237.pdf |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/edn3.237 |
container_title |
Environmental DNA |
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1765999583198969856 |