Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly
Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high‐resolution small subunit rRNA gene sequencing to explore arc...
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ftoslouniv:oai:www.duo.uio.no:10852/85173 2023-05-15T15:07:49+02:00 Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander 2020-12-24T10:01:31Z http://hdl.handle.net/10852/85173 http://urn.nb.no/URN:NBN:no-87932 https://doi.org/10.1111/1462-2920.15058 EN eng http://urn.nb.no/URN:NBN:no-87932 Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander . Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly. Environmental Microbiology. 2020 http://hdl.handle.net/10852/85173 1863161 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental Microbiology&rft.volume=&rft.spage=&rft.date=2020 Environmental Microbiology 22 8 3158 3171 https://doi.org/10.1111/1462-2920.15058 URN:NBN:no-87932 Fulltext https://www.duo.uio.no/bitstream/handle/10852/85173/2/Archaea%2Bin%2Bboreal%2BSwedish%2Blakes-1462-2920.15058.pdf Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ CC-BY 1462-2912 Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2020 ftoslouniv https://doi.org/10.1111/1462-2920.15058 2021-04-21T22:30:58Z Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high‐resolution small subunit rRNA gene sequencing to explore archaeal diversity in 109 Swedish lakes and correlate archaeal community assembly mechanisms to large‐scale latitudinal, climatic (nemoral to arctic) and nutrient (oligotrophic to eutrophic) gradients. Sequencing with universal primers showed the contribution of archaea was on average 0.8% but increased up to 1.5% of the three domains in forest lakes. Archaea‐specific sequencing revealed that freshwater archaeal diversity could be partly explained by lake variables associated with nutrient status. Combined with deterministic co‐occurrence patterns this finding suggests that ecological drift is overridden by environmental sorting, as well as other deterministic processes such as biogeographic and evolutionary history, leading to lake‐specific archaeal biodiversity. Acetoclastic, hydrogenotrophic and methylotrophic methanogens as well as ammonia‐oxidizing archaea were frequently detected across the lakes. Archaea‐specific sequencing also revealed representatives of Woesearchaeota and other phyla of the DPANN superphylum. This study adds to our understanding of the ecological range of key archaea in freshwaters and links these taxa to hypotheses about processes governing biogeochemical cycles in lakes. Article in Journal/Newspaper Arctic Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Arctic Environmental Microbiology 22 8 3158 3171 |
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Open Polar |
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Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
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ftoslouniv |
language |
English |
description |
Despite their key role in biogeochemical processes, particularly the methane cycle, archaea are widely underrepresented in molecular surveys because of their lower abundance compared with bacteria and eukaryotes. Here, we use parallel high‐resolution small subunit rRNA gene sequencing to explore archaeal diversity in 109 Swedish lakes and correlate archaeal community assembly mechanisms to large‐scale latitudinal, climatic (nemoral to arctic) and nutrient (oligotrophic to eutrophic) gradients. Sequencing with universal primers showed the contribution of archaea was on average 0.8% but increased up to 1.5% of the three domains in forest lakes. Archaea‐specific sequencing revealed that freshwater archaeal diversity could be partly explained by lake variables associated with nutrient status. Combined with deterministic co‐occurrence patterns this finding suggests that ecological drift is overridden by environmental sorting, as well as other deterministic processes such as biogeographic and evolutionary history, leading to lake‐specific archaeal biodiversity. Acetoclastic, hydrogenotrophic and methylotrophic methanogens as well as ammonia‐oxidizing archaea were frequently detected across the lakes. Archaea‐specific sequencing also revealed representatives of Woesearchaeota and other phyla of the DPANN superphylum. This study adds to our understanding of the ecological range of key archaea in freshwaters and links these taxa to hypotheses about processes governing biogeochemical cycles in lakes. |
format |
Article in Journal/Newspaper |
author |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander |
spellingShingle |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
author_facet |
Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander |
author_sort |
Juottonen, Heli |
title |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_short |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_full |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_fullStr |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_full_unstemmed |
Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly |
title_sort |
archaea in boreal swedish lakes are diverse, dominated by woesearchaeota and follow deterministic community assembly |
publishDate |
2020 |
url |
http://hdl.handle.net/10852/85173 http://urn.nb.no/URN:NBN:no-87932 https://doi.org/10.1111/1462-2920.15058 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
1462-2912 |
op_relation |
http://urn.nb.no/URN:NBN:no-87932 Juottonen, Heli Fontaine, Laurent Wurzbacher, Christian Drakare, Stina Peura, Sari Eiler, Alexander . Archaea in boreal Swedish lakes are diverse, dominated by Woesearchaeota and follow deterministic community assembly. Environmental Microbiology. 2020 http://hdl.handle.net/10852/85173 1863161 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental Microbiology&rft.volume=&rft.spage=&rft.date=2020 Environmental Microbiology 22 8 3158 3171 https://doi.org/10.1111/1462-2920.15058 URN:NBN:no-87932 Fulltext https://www.duo.uio.no/bitstream/handle/10852/85173/2/Archaea%2Bin%2Bboreal%2BSwedish%2Blakes-1462-2920.15058.pdf |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/1462-2920.15058 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
8 |
container_start_page |
3158 |
op_container_end_page |
3171 |
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1766339246810988544 |