The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish
Palmatine (PALM) and berberine (BERB) are widely identified isoquinoline alkaloids among the representatives of the Berberidaceae botanical family. The antiseizure activity of BERB was shown previously in experimental epilepsy models. We assessed the effect of PALM in a pentylenetetrazole (PTZ)-indu...
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2020
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ftoslouniv:oai:www.duo.uio.no:10852/84030 2023-05-15T18:19:41+02:00 The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish Gawel, Kinga Aurelia Kukula-Koch, Wirginia Nieoczym, Dorota Stępnik, Katarzyna van der Ent, Wietske Banono, Nancy Saana Tarabasz, Dominik Turski, Waldemar A. Esguerra, Camila 2020-10-16T15:36:17Z http://hdl.handle.net/10852/84030 http://urn.nb.no/URN:NBN:no-86767 https://doi.org/10.3390/cells9051233 EN eng NFR/187615 http://urn.nb.no/URN:NBN:no-86767 Gawel, Kinga Aurelia Kukula-Koch, Wirginia Nieoczym, Dorota Stępnik, Katarzyna van der Ent, Wietske Banono, Nancy Saana Tarabasz, Dominik Turski, Waldemar A. Esguerra, Camila . The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish. Cells. 2020, 9(5) http://hdl.handle.net/10852/84030 1840232 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.volume=9&rft.spage=&rft.date=2020 Cells 9 5 https://doi.org/10.3390/cells9051233 URN:NBN:no-86767 Fulltext https://www.duo.uio.no/bitstream/handle/10852/84030/1/Gawel%2B2020%2BPalmatine%2BPTZ%2Bseizures%2Bzebrafish.pdf Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ CC-BY 2073-4409 Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2020 ftoslouniv https://doi.org/10.3390/cells9051233 2021-03-17T23:31:00Z Palmatine (PALM) and berberine (BERB) are widely identified isoquinoline alkaloids among the representatives of the Berberidaceae botanical family. The antiseizure activity of BERB was shown previously in experimental epilepsy models. We assessed the effect of PALM in a pentylenetetrazole (PTZ)-induced seizure assay in zebrafish, with BERB as an active reference compound. Both alkaloids were isolated from the methanolic root extract of Berberis sibirica by counter-current chromatography, and their ability to cross the blood-brain barrier was determined via quantitative structure-activity relationship assay. PALM exerted antiseizure activity, as confirmed by electroencephalographic analysis, and decreased c-fos and bdnf levels in PTZ-treated larvae. In a behavioral assay, PALM dose-dependently decreased PTZ-induced hyperlocomotion. The combination of PALM and BERB in ED16 doses revealed hyperadditive activity towards PTZ-induced hyperlocomotion. Notably, we have indicated that both alkaloids may exert their anticonvulsant activity through different mechanisms of action. Additionally, the combination of both alkaloids in a 1:2.17 ratio (PALM: BERB) mimicked the activity of the pure extract, which indicates that these two active compounds are responsible for its anticonvulsive activity. In conclusion, our study reveals for the first time the anticonvulsant activity of PALM and suggests the combination of PALM and BERB may have higher therapeutic value than separate usage of these compounds. Article in Journal/Newspaper Sibirica Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Cells 9 5 1233 |
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Open Polar |
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Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
Palmatine (PALM) and berberine (BERB) are widely identified isoquinoline alkaloids among the representatives of the Berberidaceae botanical family. The antiseizure activity of BERB was shown previously in experimental epilepsy models. We assessed the effect of PALM in a pentylenetetrazole (PTZ)-induced seizure assay in zebrafish, with BERB as an active reference compound. Both alkaloids were isolated from the methanolic root extract of Berberis sibirica by counter-current chromatography, and their ability to cross the blood-brain barrier was determined via quantitative structure-activity relationship assay. PALM exerted antiseizure activity, as confirmed by electroencephalographic analysis, and decreased c-fos and bdnf levels in PTZ-treated larvae. In a behavioral assay, PALM dose-dependently decreased PTZ-induced hyperlocomotion. The combination of PALM and BERB in ED16 doses revealed hyperadditive activity towards PTZ-induced hyperlocomotion. Notably, we have indicated that both alkaloids may exert their anticonvulsant activity through different mechanisms of action. Additionally, the combination of both alkaloids in a 1:2.17 ratio (PALM: BERB) mimicked the activity of the pure extract, which indicates that these two active compounds are responsible for its anticonvulsive activity. In conclusion, our study reveals for the first time the anticonvulsant activity of PALM and suggests the combination of PALM and BERB may have higher therapeutic value than separate usage of these compounds. |
format |
Article in Journal/Newspaper |
author |
Gawel, Kinga Aurelia Kukula-Koch, Wirginia Nieoczym, Dorota Stępnik, Katarzyna van der Ent, Wietske Banono, Nancy Saana Tarabasz, Dominik Turski, Waldemar A. Esguerra, Camila |
spellingShingle |
Gawel, Kinga Aurelia Kukula-Koch, Wirginia Nieoczym, Dorota Stępnik, Katarzyna van der Ent, Wietske Banono, Nancy Saana Tarabasz, Dominik Turski, Waldemar A. Esguerra, Camila The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish |
author_facet |
Gawel, Kinga Aurelia Kukula-Koch, Wirginia Nieoczym, Dorota Stępnik, Katarzyna van der Ent, Wietske Banono, Nancy Saana Tarabasz, Dominik Turski, Waldemar A. Esguerra, Camila |
author_sort |
Gawel, Kinga Aurelia |
title |
The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish |
title_short |
The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish |
title_full |
The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish |
title_fullStr |
The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish |
title_full_unstemmed |
The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish |
title_sort |
influence of palmatine isolated from berberis sibirica radix on pentylenetetrazole-induced seizures in zebrafish |
publishDate |
2020 |
url |
http://hdl.handle.net/10852/84030 http://urn.nb.no/URN:NBN:no-86767 https://doi.org/10.3390/cells9051233 |
genre |
Sibirica |
genre_facet |
Sibirica |
op_source |
2073-4409 |
op_relation |
NFR/187615 http://urn.nb.no/URN:NBN:no-86767 Gawel, Kinga Aurelia Kukula-Koch, Wirginia Nieoczym, Dorota Stępnik, Katarzyna van der Ent, Wietske Banono, Nancy Saana Tarabasz, Dominik Turski, Waldemar A. Esguerra, Camila . The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish. Cells. 2020, 9(5) http://hdl.handle.net/10852/84030 1840232 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.volume=9&rft.spage=&rft.date=2020 Cells 9 5 https://doi.org/10.3390/cells9051233 URN:NBN:no-86767 Fulltext https://www.duo.uio.no/bitstream/handle/10852/84030/1/Gawel%2B2020%2BPalmatine%2BPTZ%2Bseizures%2Bzebrafish.pdf |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/cells9051233 |
container_title |
Cells |
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9 |
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5 |
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1233 |
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1766196880182607872 |