Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua)
Atlantic cod (Gadus morhua) represents a unique immune system among teleost fish, making it a species of interest for immunological studies, and especially for investigating the evolutionary history of immune gene families. The interferon regulatory factor (IRF) gene family encodes transcription fac...
Published in: | Developmental & Comparative Immunology |
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ftoslouniv:oai:www.duo.uio.no:10852/78018 2023-05-15T15:26:26+02:00 Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua) Inkpen, Sabrina Solbakken, Monica Hongrø Jentoft, Sissel Eslamloo, Khalil Rise, Matthew L. 2019-07-02T13:22:04Z http://hdl.handle.net/10852/78018 http://urn.nb.no/URN:NBN:no-81119 https://doi.org/10.1016/j.dci.2019.03.015 EN eng NFR/222378 http://urn.nb.no/URN:NBN:no-81119 Inkpen, Sabrina Solbakken, Monica Hongrø Jentoft, Sissel Eslamloo, Khalil Rise, Matthew L. . Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua). Developmental and Comparative Immunology. 2019, 98, 166-180 http://hdl.handle.net/10852/78018 1709384 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Developmental and Comparative Immunology&rft.volume=98&rft.spage=166&rft.date=2019 Developmental and Comparative Immunology 98 166 180 https://doi.org/10.1016/j.dci.2019.03.015 URN:NBN:no-81119 Fulltext https://www.duo.uio.no/bitstream/handle/10852/78018/1/Full%2Bcharacterization%2Band%2Btranscript%2Bexpression%2B-%2Bpost-print.pdf Attribution-NonCommercial-NoDerivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/ CC-BY-NC-ND 0145-305X Journal article Tidsskriftartikkel Peer reviewed AcceptedVersion 2019 ftoslouniv https://doi.org/10.1016/j.dci.2019.03.015 2020-07-22T22:29:13Z Atlantic cod (Gadus morhua) represents a unique immune system among teleost fish, making it a species of interest for immunological studies, and especially for investigating the evolutionary history of immune gene families. The interferon regulatory factor (IRF) gene family encodes transcription factors which function in the interferon pathway, but also in areas including leukocyte differentiation, cell growth, autoimmunity, and development. We previously characterized several IRF family members in Atlantic cod (Irf4a, Irf4b, Irf7, Irf8, and two Irf10 splice variants) at the cDNA and putative amino acid levels, and in the current study we took advantage of the new and improved Atlantic cod genome assembly in combination with rapid amplification of cDNA ends (RACE) to characterize the remaining family members (i.e. Irf3, Irf5, Irf6, Irf9, and two Irf2 splice variants). Real-time quantitative PCR (QPCR) was used to investigate constitutive expression of all IRF transcripts during embryonic development, suggesting several putative maternal transcripts, and potential stage-specific roles. QPCR studies also showed 11 of 13 transcripts were responsive to stimulation with poly(I:C), while 6 of 13 transcripts were responsive to lipopolysaccharide (LPS) in Atlantic cod head kidney macrophages, indicating roles for cod IRF family members in both antiviral and antibacterial responses. This study is the first to investigate expression of the complete IRF family in Atlantic cod, and suggests potential novel roles for several of these transcription factors within immunity as well as in early development of this species. Article in Journal/Newspaper atlantic cod Gadus morhua Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Developmental & Comparative Immunology 98 166 180 |
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Open Polar |
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Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
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ftoslouniv |
language |
English |
description |
Atlantic cod (Gadus morhua) represents a unique immune system among teleost fish, making it a species of interest for immunological studies, and especially for investigating the evolutionary history of immune gene families. The interferon regulatory factor (IRF) gene family encodes transcription factors which function in the interferon pathway, but also in areas including leukocyte differentiation, cell growth, autoimmunity, and development. We previously characterized several IRF family members in Atlantic cod (Irf4a, Irf4b, Irf7, Irf8, and two Irf10 splice variants) at the cDNA and putative amino acid levels, and in the current study we took advantage of the new and improved Atlantic cod genome assembly in combination with rapid amplification of cDNA ends (RACE) to characterize the remaining family members (i.e. Irf3, Irf5, Irf6, Irf9, and two Irf2 splice variants). Real-time quantitative PCR (QPCR) was used to investigate constitutive expression of all IRF transcripts during embryonic development, suggesting several putative maternal transcripts, and potential stage-specific roles. QPCR studies also showed 11 of 13 transcripts were responsive to stimulation with poly(I:C), while 6 of 13 transcripts were responsive to lipopolysaccharide (LPS) in Atlantic cod head kidney macrophages, indicating roles for cod IRF family members in both antiviral and antibacterial responses. This study is the first to investigate expression of the complete IRF family in Atlantic cod, and suggests potential novel roles for several of these transcription factors within immunity as well as in early development of this species. |
format |
Article in Journal/Newspaper |
author |
Inkpen, Sabrina Solbakken, Monica Hongrø Jentoft, Sissel Eslamloo, Khalil Rise, Matthew L. |
spellingShingle |
Inkpen, Sabrina Solbakken, Monica Hongrø Jentoft, Sissel Eslamloo, Khalil Rise, Matthew L. Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua) |
author_facet |
Inkpen, Sabrina Solbakken, Monica Hongrø Jentoft, Sissel Eslamloo, Khalil Rise, Matthew L. |
author_sort |
Inkpen, Sabrina |
title |
Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua) |
title_short |
Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua) |
title_full |
Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua) |
title_fullStr |
Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua) |
title_full_unstemmed |
Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua) |
title_sort |
full characterization and transcript expression profiling of the interferon regulatory factor (irf) gene family in atlantic cod (gadus morhua) |
publishDate |
2019 |
url |
http://hdl.handle.net/10852/78018 http://urn.nb.no/URN:NBN:no-81119 https://doi.org/10.1016/j.dci.2019.03.015 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
0145-305X |
op_relation |
NFR/222378 http://urn.nb.no/URN:NBN:no-81119 Inkpen, Sabrina Solbakken, Monica Hongrø Jentoft, Sissel Eslamloo, Khalil Rise, Matthew L. . Full characterization and transcript expression profiling of the interferon regulatory factor (IRF) gene family in Atlantic cod (Gadus morhua). Developmental and Comparative Immunology. 2019, 98, 166-180 http://hdl.handle.net/10852/78018 1709384 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Developmental and Comparative Immunology&rft.volume=98&rft.spage=166&rft.date=2019 Developmental and Comparative Immunology 98 166 180 https://doi.org/10.1016/j.dci.2019.03.015 URN:NBN:no-81119 Fulltext https://www.duo.uio.no/bitstream/handle/10852/78018/1/Full%2Bcharacterization%2Band%2Btranscript%2Bexpression%2B-%2Bpost-print.pdf |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.dci.2019.03.015 |
container_title |
Developmental & Comparative Immunology |
container_volume |
98 |
container_start_page |
166 |
op_container_end_page |
180 |
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1766356921106825216 |