In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.)
Bisphenol A (BPA) and tetrabromobisphenol A (TBBPA) are widely used industrial chemicals, ubiquitously present in the environment. While BPA is a well-known endocrine disruptor and able to affect all levels of the teleost reproductive axis, information regarding TBBPA on this subject is very limited...
Published in: | Fishes |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | http://hdl.handle.net/10852/76905 http://urn.nb.no/URN:NBN:no-79982 https://doi.org/10.3390/fishes4030048 |
id |
ftoslouniv:oai:www.duo.uio.no:10852/76905 |
---|---|
record_format |
openpolar |
spelling |
ftoslouniv:oai:www.duo.uio.no:10852/76905 2023-05-15T15:26:58+02:00 In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.) von Krogh, Kristine Weltzien, Finn-Arne Haug, Trude Nourizadeh-Lillabadi, Rasoul Ropstad, Erik 2019-12-09T15:34:03Z http://hdl.handle.net/10852/76905 http://urn.nb.no/URN:NBN:no-79982 https://doi.org/10.3390/fishes4030048 EN eng NFR/184851 NFR/191825 http://urn.nb.no/URN:NBN:no-79982 von Krogh, Kristine Weltzien, Finn-Arne Haug, Trude Nourizadeh-Lillabadi, Rasoul Ropstad, Erik . In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.). Fishes. 2019, 4(3) http://hdl.handle.net/10852/76905 1758423 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Fishes&rft.volume=4&rft.spage=&rft.date=2019 Fishes 4 3 https://doi.org/10.3390/fishes4030048 URN:NBN:no-79982 Fulltext https://www.duo.uio.no/bitstream/handle/10852/76905/2/fishes-04-00048.pdf Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ CC-BY 2410-3888 Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2019 ftoslouniv https://doi.org/10.3390/fishes4030048 2020-06-21T08:54:40Z Bisphenol A (BPA) and tetrabromobisphenol A (TBBPA) are widely used industrial chemicals, ubiquitously present in the environment. While BPA is a well-known endocrine disruptor and able to affect all levels of the teleost reproductive axis, information regarding TBBPA on this subject is very limited. Using primary cultures from Atlantic cod (Gadus morhua), the present study was aimed at investigating potential direct effects of acute (72 h) BPA and TBBPA exposure on cell viability and the expression of reproductive-relevant genes in the pituitary. The results revealed that both bisphenols stimulate cell viability in terms of metabolic activity and membrane integrity at environmentally relevant concentrations. BPA had no direct effects on gonadotropin gene expression, but enhanced the expression of gonadotropin-releasing hormone (GnRH) receptor 2a, the main gonadotropin modulator in Atlantic cod. In contrast, TBBPA increased gonadotropin transcript levels but had no effect on GnRH receptor mRNA. In conclusion, both anthropogenic compounds display endocrine disruptive properties and are able to directly interfere with gene expression related to reproductive function in cod pituitary cells at environmentally relevant concentrations in vitro. Article in Journal/Newspaper atlantic cod Gadus morhua Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Fishes 4 3 48 |
institution |
Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
Bisphenol A (BPA) and tetrabromobisphenol A (TBBPA) are widely used industrial chemicals, ubiquitously present in the environment. While BPA is a well-known endocrine disruptor and able to affect all levels of the teleost reproductive axis, information regarding TBBPA on this subject is very limited. Using primary cultures from Atlantic cod (Gadus morhua), the present study was aimed at investigating potential direct effects of acute (72 h) BPA and TBBPA exposure on cell viability and the expression of reproductive-relevant genes in the pituitary. The results revealed that both bisphenols stimulate cell viability in terms of metabolic activity and membrane integrity at environmentally relevant concentrations. BPA had no direct effects on gonadotropin gene expression, but enhanced the expression of gonadotropin-releasing hormone (GnRH) receptor 2a, the main gonadotropin modulator in Atlantic cod. In contrast, TBBPA increased gonadotropin transcript levels but had no effect on GnRH receptor mRNA. In conclusion, both anthropogenic compounds display endocrine disruptive properties and are able to directly interfere with gene expression related to reproductive function in cod pituitary cells at environmentally relevant concentrations in vitro. |
format |
Article in Journal/Newspaper |
author |
von Krogh, Kristine Weltzien, Finn-Arne Haug, Trude Nourizadeh-Lillabadi, Rasoul Ropstad, Erik |
spellingShingle |
von Krogh, Kristine Weltzien, Finn-Arne Haug, Trude Nourizadeh-Lillabadi, Rasoul Ropstad, Erik In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.) |
author_facet |
von Krogh, Kristine Weltzien, Finn-Arne Haug, Trude Nourizadeh-Lillabadi, Rasoul Ropstad, Erik |
author_sort |
von Krogh, Kristine |
title |
In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.) |
title_short |
In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.) |
title_full |
In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.) |
title_fullStr |
In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.) |
title_full_unstemmed |
In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.) |
title_sort |
in vitro effects of bisphenol a and tetrabromobisphenol a on cell viability and reproduction-related gene expression in pituitaries from sexually maturing atlantic cod (gadus morhua l.) |
publishDate |
2019 |
url |
http://hdl.handle.net/10852/76905 http://urn.nb.no/URN:NBN:no-79982 https://doi.org/10.3390/fishes4030048 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
2410-3888 |
op_relation |
NFR/184851 NFR/191825 http://urn.nb.no/URN:NBN:no-79982 von Krogh, Kristine Weltzien, Finn-Arne Haug, Trude Nourizadeh-Lillabadi, Rasoul Ropstad, Erik . In Vitro Effects of Bisphenol A and Tetrabromobisphenol A on Cell Viability and Reproduction-Related Gene Expression in Pituitaries from Sexually Maturing Atlantic Cod (Gadus morhua L.). Fishes. 2019, 4(3) http://hdl.handle.net/10852/76905 1758423 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Fishes&rft.volume=4&rft.spage=&rft.date=2019 Fishes 4 3 https://doi.org/10.3390/fishes4030048 URN:NBN:no-79982 Fulltext https://www.duo.uio.no/bitstream/handle/10852/76905/2/fishes-04-00048.pdf |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/fishes4030048 |
container_title |
Fishes |
container_volume |
4 |
container_issue |
3 |
container_start_page |
48 |
_version_ |
1766357421731610624 |