Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation
Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates. We report the characterization of C5 cDNA and protein from Atlantic salmon (Salmo salar), a teleost fish species of high importance in aquaculture. The C5 cDNA cloned from liver is 5079 nucleoti...
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ftoslouniv:oai:www.duo.uio.no:10852/74142 2023-05-15T15:30:59+02:00 Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation Johansen, Wenche Grove, Søren Anonsen, Jan Haug Moen, Anders Agusti-Ridaura, Celia Azar, Amir Sefidmouy Strætkvern, Knut Olav 2019-06-27T10:23:22Z http://hdl.handle.net/10852/74142 http://urn.nb.no/URN:NBN:no-77253 https://doi.org/10.1016/j.dci.2019.103424 EN eng http://urn.nb.no/URN:NBN:no-77253 Johansen, Wenche Grove, Søren Anonsen, Jan Haug Moen, Anders Agusti-Ridaura, Celia Azar, Amir Sefidmouy Strætkvern, Knut Olav . Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation. Developmental and Comparative Immunology. 2019, 100 http://hdl.handle.net/10852/74142 1708211 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Developmental and Comparative Immunology&rft.volume=100&rft.spage=&rft.date=2019 Developmental and Comparative Immunology 100 https://doi.org/10.1016/j.dci.2019.103424 URN:NBN:no-77253 Fulltext https://www.duo.uio.no/bitstream/handle/10852/74142/2/1-s2.0-S0145305X19300254-main.pdf Attribution-NonCommercial-NoDerivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/ CC-BY-NC-ND 0145-305X Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2019 ftoslouniv https://doi.org/10.1016/j.dci.2019.103424 2020-06-21T08:53:53Z Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates. We report the characterization of C5 cDNA and protein from Atlantic salmon (Salmo salar), a teleost fish species of high importance in aquaculture. The C5 cDNA cloned from liver is 5079 nucleotides long, whose translation product has a molecular weight of 190 kDa, with the classical β-α orientation and motifs/sites for β-α cleavage (678RPKR681) and cleavage by C5 convertases (R758). Mass spectrometric analysis show a single N-linked, biantennary, complex glycan at N1125. Moreover, the N-linked glycan displays an unusual modification in the form of acetylated sialic acid residues. Three anti-C5 antisera produced in mice using purified C5 worked in immunohistochemical analyses of formalin fixed liver tissue. The purification method, whereby inactive and activated (C5b) forms were isolated, opens for interesting studies on the complement function in fish, including possible connection to stress, disease and glycosylation. Article in Journal/Newspaper Atlantic salmon Salmo salar Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Developmental & Comparative Immunology 100 103424 |
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Open Polar |
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Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
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ftoslouniv |
language |
English |
description |
Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates. We report the characterization of C5 cDNA and protein from Atlantic salmon (Salmo salar), a teleost fish species of high importance in aquaculture. The C5 cDNA cloned from liver is 5079 nucleotides long, whose translation product has a molecular weight of 190 kDa, with the classical β-α orientation and motifs/sites for β-α cleavage (678RPKR681) and cleavage by C5 convertases (R758). Mass spectrometric analysis show a single N-linked, biantennary, complex glycan at N1125. Moreover, the N-linked glycan displays an unusual modification in the form of acetylated sialic acid residues. Three anti-C5 antisera produced in mice using purified C5 worked in immunohistochemical analyses of formalin fixed liver tissue. The purification method, whereby inactive and activated (C5b) forms were isolated, opens for interesting studies on the complement function in fish, including possible connection to stress, disease and glycosylation. |
format |
Article in Journal/Newspaper |
author |
Johansen, Wenche Grove, Søren Anonsen, Jan Haug Moen, Anders Agusti-Ridaura, Celia Azar, Amir Sefidmouy Strætkvern, Knut Olav |
spellingShingle |
Johansen, Wenche Grove, Søren Anonsen, Jan Haug Moen, Anders Agusti-Ridaura, Celia Azar, Amir Sefidmouy Strætkvern, Knut Olav Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation |
author_facet |
Johansen, Wenche Grove, Søren Anonsen, Jan Haug Moen, Anders Agusti-Ridaura, Celia Azar, Amir Sefidmouy Strætkvern, Knut Olav |
author_sort |
Johansen, Wenche |
title |
Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation |
title_short |
Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation |
title_full |
Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation |
title_fullStr |
Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation |
title_full_unstemmed |
Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation |
title_sort |
complement factor c5 in atlantic salmon (salmo salar): characterization of cdna, protein and glycosylation |
publishDate |
2019 |
url |
http://hdl.handle.net/10852/74142 http://urn.nb.no/URN:NBN:no-77253 https://doi.org/10.1016/j.dci.2019.103424 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
0145-305X |
op_relation |
http://urn.nb.no/URN:NBN:no-77253 Johansen, Wenche Grove, Søren Anonsen, Jan Haug Moen, Anders Agusti-Ridaura, Celia Azar, Amir Sefidmouy Strætkvern, Knut Olav . Complement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation. Developmental and Comparative Immunology. 2019, 100 http://hdl.handle.net/10852/74142 1708211 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Developmental and Comparative Immunology&rft.volume=100&rft.spage=&rft.date=2019 Developmental and Comparative Immunology 100 https://doi.org/10.1016/j.dci.2019.103424 URN:NBN:no-77253 Fulltext https://www.duo.uio.no/bitstream/handle/10852/74142/2/1-s2.0-S0145305X19300254-main.pdf |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.dci.2019.103424 |
container_title |
Developmental & Comparative Immunology |
container_volume |
100 |
container_start_page |
103424 |
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1766361477622530048 |