DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas
Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrat...
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier Science
2017
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Online Access: | http://hdl.handle.net/10852/64838 http://urn.nb.no/URN:NBN:no-67373 https://doi.org/10.1016/j.marenvres.2016.01.001 |
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ftoslouniv:oai:www.duo.uio.no:10852/64838 2023-05-15T16:45:06+02:00 DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas Hylland, Ketil Skei, Bjørn Borge Brunborg, Gunnar Lang, Thomas Gubbins, Matthew J. Le Goff, Jérémie Burgeot, Thierry 2017-06-13T23:06:09Z http://hdl.handle.net/10852/64838 http://urn.nb.no/URN:NBN:no-67373 https://doi.org/10.1016/j.marenvres.2016.01.001 EN eng Elsevier Science http://urn.nb.no/URN:NBN:no-67373 Hylland, Ketil Skei, Bjørn Borge Brunborg, Gunnar Lang, Thomas Gubbins, Matthew J. Le Goff, Jérémie Burgeot, Thierry . DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas. Marine Environmental Research. 2017, 124, 54-60 http://hdl.handle.net/10852/64838 1475881 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Marine Environmental Research&rft.volume=124&rft.spage=54&rft.date=2017 Marine Environmental Research 124 54 60 http://dx.doi.org/10.1016/j.marenvres.2016.01.001 URN:NBN:no-67373 Fulltext https://www.duo.uio.no/bitstream/handle/10852/64838/2/hylland%2Bgenotoxicity.pdf Attribution-NonCommercial-NoDerivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/ CC-BY-NC-ND 0141-1136 Journal article Tidsskriftartikkel Peer reviewed AcceptedVersion 2017 ftoslouniv https://doi.org/10.1016/j.marenvres.2016.01.001 2020-06-21T08:51:41Z Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from the Dogger Bank. The highest levels were observed in dab from the Firth of Forth, Ekofisk and the German Bight. Hepatic DNA adducts in dab were highest at Ekofisk, in the Baltic and Dogger Bank, below detection limit in dab from Iceland and low in dab from the Firth of Forth and German Bight. There was large variation in DNA strand breaks between locations and individuals for haddock, particularly from Iceland. Adduct concentrations were elevated in haddock from both Iceland and the Firth of Forth. A general linear model (GLM) suggested that, in addition to location, the size of dab and its general condition contributed to explaining the observed variability in DNA strand breaks. A GLM for adducts in dab similarly allocated most of the variability to location, but with a possible contribution from CYP1A activity. There were no apparent differences between male and female dab for any of the methods. There was no obvious relationship between strand breaks and adducts in the same fish although dab from Ekofisk and Iceland had respectively high and low responses using both methods. The results from this large-scale study showed pollution-related genotoxicity for dab, that fish blood samples can be conserved prior to comet analyses and that there are clear species differences in genotoxic responses even when collected at the same location. Article in Journal/Newspaper Iceland Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Dogger Bank ENVELOPE(2.333,2.333,54.833,54.833) Marine Environmental Research 124 54 60 |
institution |
Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from the Dogger Bank. The highest levels were observed in dab from the Firth of Forth, Ekofisk and the German Bight. Hepatic DNA adducts in dab were highest at Ekofisk, in the Baltic and Dogger Bank, below detection limit in dab from Iceland and low in dab from the Firth of Forth and German Bight. There was large variation in DNA strand breaks between locations and individuals for haddock, particularly from Iceland. Adduct concentrations were elevated in haddock from both Iceland and the Firth of Forth. A general linear model (GLM) suggested that, in addition to location, the size of dab and its general condition contributed to explaining the observed variability in DNA strand breaks. A GLM for adducts in dab similarly allocated most of the variability to location, but with a possible contribution from CYP1A activity. There were no apparent differences between male and female dab for any of the methods. There was no obvious relationship between strand breaks and adducts in the same fish although dab from Ekofisk and Iceland had respectively high and low responses using both methods. The results from this large-scale study showed pollution-related genotoxicity for dab, that fish blood samples can be conserved prior to comet analyses and that there are clear species differences in genotoxic responses even when collected at the same location. |
format |
Article in Journal/Newspaper |
author |
Hylland, Ketil Skei, Bjørn Borge Brunborg, Gunnar Lang, Thomas Gubbins, Matthew J. Le Goff, Jérémie Burgeot, Thierry |
spellingShingle |
Hylland, Ketil Skei, Bjørn Borge Brunborg, Gunnar Lang, Thomas Gubbins, Matthew J. Le Goff, Jérémie Burgeot, Thierry DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas |
author_facet |
Hylland, Ketil Skei, Bjørn Borge Brunborg, Gunnar Lang, Thomas Gubbins, Matthew J. Le Goff, Jérémie Burgeot, Thierry |
author_sort |
Hylland, Ketil |
title |
DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas |
title_short |
DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas |
title_full |
DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas |
title_fullStr |
DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas |
title_full_unstemmed |
DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas |
title_sort |
dna damage in dab (limanda limanda) and haddock (melanogrammus aeglefinus) from european seas |
publisher |
Elsevier Science |
publishDate |
2017 |
url |
http://hdl.handle.net/10852/64838 http://urn.nb.no/URN:NBN:no-67373 https://doi.org/10.1016/j.marenvres.2016.01.001 |
long_lat |
ENVELOPE(2.333,2.333,54.833,54.833) |
geographic |
Dogger Bank |
geographic_facet |
Dogger Bank |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
0141-1136 |
op_relation |
http://urn.nb.no/URN:NBN:no-67373 Hylland, Ketil Skei, Bjørn Borge Brunborg, Gunnar Lang, Thomas Gubbins, Matthew J. Le Goff, Jérémie Burgeot, Thierry . DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas. Marine Environmental Research. 2017, 124, 54-60 http://hdl.handle.net/10852/64838 1475881 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Marine Environmental Research&rft.volume=124&rft.spage=54&rft.date=2017 Marine Environmental Research 124 54 60 http://dx.doi.org/10.1016/j.marenvres.2016.01.001 URN:NBN:no-67373 Fulltext https://www.duo.uio.no/bitstream/handle/10852/64838/2/hylland%2Bgenotoxicity.pdf |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 International https://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.marenvres.2016.01.001 |
container_title |
Marine Environmental Research |
container_volume |
124 |
container_start_page |
54 |
op_container_end_page |
60 |
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1766035321510690816 |