Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon
Viral diseases pose a significant threat to the productivity in aquaculture. Heart- and skeletal muscle inflammation (HSMI) is an emerging disease in Atlantic salmon (Salmo salar) farming. HSMI is associated with Piscine orthoreovirus (PRV) infection, but PRV is ubiquitous in farmed Atlantic salmon...
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ftoslouniv:oai:www.duo.uio.no:10852/62291 2023-05-15T15:29:29+02:00 Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon Wessel, Øystein Braaen, Stine Alarcon, Marta Haatveit, Hanne Merethe Roos, Norbert Markussen, Turhan Tengs, Torstein Dahle, Maria Krudtaa Rimstad, Espen 2017-08-29T07:57:35Z http://hdl.handle.net/10852/62291 http://urn.nb.no/URN:NBN:no-64878 https://doi.org/10.1371/journal.pone.0183781 EN eng Public Library of Science (PLoS) http://urn.nb.no/URN:NBN:no-64878 Wessel, Øystein Braaen, Stine Alarcon, Marta Haatveit, Hanne Merethe Roos, Norbert Markussen, Turhan Tengs, Torstein Dahle, Maria Krudtaa Rimstad, Espen . Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon. PLoS ONE. 2017, 12(8) http://hdl.handle.net/10852/62291 1489248 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=12&rft.spage=&rft.date=2017 PLoS ONE 12 8 http://dx.doi.org/10.1371/journal.pone.0183781 URN:NBN:no-64878 Fulltext https://www.duo.uio.no/bitstream/handle/10852/62291/2/wessel.pdf Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ CC-BY 1932-6203 Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2017 ftoslouniv https://doi.org/10.1371/journal.pone.0183781 2020-06-21T08:51:23Z Viral diseases pose a significant threat to the productivity in aquaculture. Heart- and skeletal muscle inflammation (HSMI) is an emerging disease in Atlantic salmon (Salmo salar) farming. HSMI is associated with Piscine orthoreovirus (PRV) infection, but PRV is ubiquitous in farmed Atlantic salmon and thus present also in apparently healthy individuals. This has brought speculations if additional etiological factors are required, and experiments focusing on the causal relationship between PRV and HSMI are highly warranted. A major bottleneck in PRV research has been the lack of cell lines that allow propagation of the virus. To bypass this, we propagated PRV in salmon, bled the fish at the peak of the infection, and purified virus particles from blood cells. Electron microscopy, western blot and high-throughput sequencing all verified the purity of the viral particles. Purified PRV particles were inoculated into naïve Atlantic salmon. The purified virus replicated in inoculated fish, spread to naïve cohabitants, and induced histopathological changes consistent with HSMI. PRV specific staining was demonstrated in the pathological lesions. A dose-dependent response was observed; a high dose of virus gave earlier peak of the viral load and development of histopathological changes compared to a lower dose, but no difference in the severity of the disease. The experiment demonstrated that PRV can be purified from blood cells, and that PRV is the etiological agent of HSMI in Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar Universitet i Oslo: Digitale utgivelser ved UiO (DUO) PLOS ONE 12 8 e0183781 |
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Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
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ftoslouniv |
language |
English |
description |
Viral diseases pose a significant threat to the productivity in aquaculture. Heart- and skeletal muscle inflammation (HSMI) is an emerging disease in Atlantic salmon (Salmo salar) farming. HSMI is associated with Piscine orthoreovirus (PRV) infection, but PRV is ubiquitous in farmed Atlantic salmon and thus present also in apparently healthy individuals. This has brought speculations if additional etiological factors are required, and experiments focusing on the causal relationship between PRV and HSMI are highly warranted. A major bottleneck in PRV research has been the lack of cell lines that allow propagation of the virus. To bypass this, we propagated PRV in salmon, bled the fish at the peak of the infection, and purified virus particles from blood cells. Electron microscopy, western blot and high-throughput sequencing all verified the purity of the viral particles. Purified PRV particles were inoculated into naïve Atlantic salmon. The purified virus replicated in inoculated fish, spread to naïve cohabitants, and induced histopathological changes consistent with HSMI. PRV specific staining was demonstrated in the pathological lesions. A dose-dependent response was observed; a high dose of virus gave earlier peak of the viral load and development of histopathological changes compared to a lower dose, but no difference in the severity of the disease. The experiment demonstrated that PRV can be purified from blood cells, and that PRV is the etiological agent of HSMI in Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Wessel, Øystein Braaen, Stine Alarcon, Marta Haatveit, Hanne Merethe Roos, Norbert Markussen, Turhan Tengs, Torstein Dahle, Maria Krudtaa Rimstad, Espen |
spellingShingle |
Wessel, Øystein Braaen, Stine Alarcon, Marta Haatveit, Hanne Merethe Roos, Norbert Markussen, Turhan Tengs, Torstein Dahle, Maria Krudtaa Rimstad, Espen Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon |
author_facet |
Wessel, Øystein Braaen, Stine Alarcon, Marta Haatveit, Hanne Merethe Roos, Norbert Markussen, Turhan Tengs, Torstein Dahle, Maria Krudtaa Rimstad, Espen |
author_sort |
Wessel, Øystein |
title |
Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon |
title_short |
Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon |
title_full |
Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon |
title_fullStr |
Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon |
title_full_unstemmed |
Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon |
title_sort |
infection with purified piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in atlantic salmon |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
http://hdl.handle.net/10852/62291 http://urn.nb.no/URN:NBN:no-64878 https://doi.org/10.1371/journal.pone.0183781 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
1932-6203 |
op_relation |
http://urn.nb.no/URN:NBN:no-64878 Wessel, Øystein Braaen, Stine Alarcon, Marta Haatveit, Hanne Merethe Roos, Norbert Markussen, Turhan Tengs, Torstein Dahle, Maria Krudtaa Rimstad, Espen . Infection with purified Piscine orthoreovirus demonstrates a causal relationship with heart and skeletal muscle inflammation in Atlantic salmon. PLoS ONE. 2017, 12(8) http://hdl.handle.net/10852/62291 1489248 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=12&rft.spage=&rft.date=2017 PLoS ONE 12 8 http://dx.doi.org/10.1371/journal.pone.0183781 URN:NBN:no-64878 Fulltext https://www.duo.uio.no/bitstream/handle/10852/62291/2/wessel.pdf |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0183781 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
8 |
container_start_page |
e0183781 |
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1766359898762772480 |