Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss)
Background Environmental DNA (eDNA) monitoring is growing increasingly popular in aquatic systems as a valuable complementary method to conventional monitoring. However, such tools have not yet been extensively applied for metazoan fish parasite monitoring. The fish ectoparasite Gyrodactylus salaris...
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ftoslouniv:oai:www.duo.uio.no:10852/61784 2023-05-15T15:29:34+02:00 Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) Rusch, Johannes C Hansen, Haakon Strand, David A Markussen, Turhan Hytterød, Sigurd Vrålstad, Trude 2018 http://hdl.handle.net/10852/61784 http://urn.nb.no/URN:NBN:no-64383 https://doi.org/10.1186/s13071-018-2916-3 eng eng Rusch, Johannes (2021) Environmental DNA (eDNA) monitoring of two different freshwater host-pathogen complexes in the interface between nature and aquaculture. Doctoral thesis. http://hdl.handle.net/10852/97860 http://hdl.handle.net/10852/97860 http://urn.nb.no/URN:NBN:no-64383 Parasites & Vectors. 2018 Jun 04;11(1):333 http://hdl.handle.net/10852/61784 1590871 https://doi.org/10.1186/s13071-018-2916-3 URN:NBN:no-64383 Fulltext https://www.duo.uio.no/bitstream/handle/10852/61784/1/13071_2018_Article_2916.pdf The Author(s); licensee BioMed Central Ltd. Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ CC-BY Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2018 ftoslouniv https://doi.org/10.1186/s13071-018-2916-3 2022-12-07T23:36:11Z Background Environmental DNA (eDNA) monitoring is growing increasingly popular in aquatic systems as a valuable complementary method to conventional monitoring. However, such tools have not yet been extensively applied for metazoan fish parasite monitoring. The fish ectoparasite Gyrodactylus salaris, introduced into Norway in 1975, has caused severe damage to Atlantic salmon populations and fisheries. Successful eradication of the parasite has been carried out in several river systems in Norway, and Atlantic salmon remain infected in only seven rivers, including three in the Drammen region. In this particular infection region, a prerequisite for treatment is to establish whether G. salaris is also present on rainbow trout upstream of the salmon migration barrier. Here, we developed and tested eDNA approaches to complement conventional surveillance methods. Methods Water samples (2 × 5 l) were filtered on-site through glass fibre filters from nine locations in the Drammen watercourse, and DNA was extracted with a CTAB protocol. We developed a qPCR assay for G. salaris targeting the nuclear ribosomal ITS1 region, and we implemented published assays targeting the mitochondrial cytochrome-b and NADH-regions for Atlantic salmon and rainbow trout, respectively. All assays were transferred successfully to droplet digital PCR (ddPCR). Results All qPCR/ddPCR assays performed well both on tissue samples and on field samples, demonstrating the applicability of eDNA detection for G. salaris, rainbow trout and Atlantic salmon in natural water systems. With ddPCR we eliminated a low cross-amplification of Gyrodactylus derjavinoides observed using qPCR, thus increasing specificity and sensitivity substantially. Duplex ddPCR for G. salaris and Atlantic salmon was successfully implemented and can be used as a method in future surveillance programs. The presence of G. salaris eDNA in the infected River Lierelva was documented, while not elsewhere. Rainbow trout eDNA was only detected at localities where the positives could be ... Article in Journal/Newspaper Atlantic salmon Salmo salar Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Norway Parasites & Vectors 11 1 |
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Open Polar |
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Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
Background Environmental DNA (eDNA) monitoring is growing increasingly popular in aquatic systems as a valuable complementary method to conventional monitoring. However, such tools have not yet been extensively applied for metazoan fish parasite monitoring. The fish ectoparasite Gyrodactylus salaris, introduced into Norway in 1975, has caused severe damage to Atlantic salmon populations and fisheries. Successful eradication of the parasite has been carried out in several river systems in Norway, and Atlantic salmon remain infected in only seven rivers, including three in the Drammen region. In this particular infection region, a prerequisite for treatment is to establish whether G. salaris is also present on rainbow trout upstream of the salmon migration barrier. Here, we developed and tested eDNA approaches to complement conventional surveillance methods. Methods Water samples (2 × 5 l) were filtered on-site through glass fibre filters from nine locations in the Drammen watercourse, and DNA was extracted with a CTAB protocol. We developed a qPCR assay for G. salaris targeting the nuclear ribosomal ITS1 region, and we implemented published assays targeting the mitochondrial cytochrome-b and NADH-regions for Atlantic salmon and rainbow trout, respectively. All assays were transferred successfully to droplet digital PCR (ddPCR). Results All qPCR/ddPCR assays performed well both on tissue samples and on field samples, demonstrating the applicability of eDNA detection for G. salaris, rainbow trout and Atlantic salmon in natural water systems. With ddPCR we eliminated a low cross-amplification of Gyrodactylus derjavinoides observed using qPCR, thus increasing specificity and sensitivity substantially. Duplex ddPCR for G. salaris and Atlantic salmon was successfully implemented and can be used as a method in future surveillance programs. The presence of G. salaris eDNA in the infected River Lierelva was documented, while not elsewhere. Rainbow trout eDNA was only detected at localities where the positives could be ... |
format |
Article in Journal/Newspaper |
author |
Rusch, Johannes C Hansen, Haakon Strand, David A Markussen, Turhan Hytterød, Sigurd Vrålstad, Trude |
spellingShingle |
Rusch, Johannes C Hansen, Haakon Strand, David A Markussen, Turhan Hytterød, Sigurd Vrålstad, Trude Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) |
author_facet |
Rusch, Johannes C Hansen, Haakon Strand, David A Markussen, Turhan Hytterød, Sigurd Vrålstad, Trude |
author_sort |
Rusch, Johannes C |
title |
Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) |
title_short |
Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) |
title_full |
Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) |
title_fullStr |
Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) |
title_full_unstemmed |
Catching the fish with the worm: a case study on eDNA detection of the monogenean parasite Gyrodactylus salaris and two of its hosts, Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) |
title_sort |
catching the fish with the worm: a case study on edna detection of the monogenean parasite gyrodactylus salaris and two of its hosts, atlantic salmon (salmo salar) and rainbow trout (oncorhynchus mykiss) |
publishDate |
2018 |
url |
http://hdl.handle.net/10852/61784 http://urn.nb.no/URN:NBN:no-64383 https://doi.org/10.1186/s13071-018-2916-3 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Rusch, Johannes (2021) Environmental DNA (eDNA) monitoring of two different freshwater host-pathogen complexes in the interface between nature and aquaculture. Doctoral thesis. http://hdl.handle.net/10852/97860 http://hdl.handle.net/10852/97860 http://urn.nb.no/URN:NBN:no-64383 Parasites & Vectors. 2018 Jun 04;11(1):333 http://hdl.handle.net/10852/61784 1590871 https://doi.org/10.1186/s13071-018-2916-3 URN:NBN:no-64383 Fulltext https://www.duo.uio.no/bitstream/handle/10852/61784/1/13071_2018_Article_2916.pdf |
op_rights |
The Author(s); licensee BioMed Central Ltd. Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13071-018-2916-3 |
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