Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)
Premise of the study: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). Methods and Results: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interp...
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ftoslouniv:oai:www.duo.uio.no:10852/49827 2023-05-15T14:51:28+02:00 Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae) Müller, Eike Hlavackova, Iva Svoen, Mildrid Elvik Alsos, Inger Greve Eidesen, Pernille Bronken 2016-01-05T15:48:01Z http://hdl.handle.net/10852/49827 http://urn.nb.no/URN:NBN:no-53546 https://doi.org/10.3732/apps.1500036 EN eng Botanical Society of America http://urn.nb.no/URN:NBN:no-53546 Müller, Eike Hlavackova, Iva Svoen, Mildrid Elvik Alsos, Inger Greve Eidesen, Pernille Bronken . Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae). Applications in Plant Sciences. 2015, 3(9), 1-4 http://hdl.handle.net/10852/49827 1306473 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Applications in Plant Sciences&rft.volume=3&rft.spage=1&rft.date=2015 Applications in Plant Sciences 3 9 1 4 http://dx.doi.org/10.3732/apps.1500036 URN:NBN:no-53546 Fulltext https://www.duo.uio.no/bitstream/handle/10852/49827/1/1306473.pdf Attribution-NonCommercial-ShareAlike 3.0 Unported http://creativecommons.org/licenses/by-nc-sa/3.0/ CC-BY-NC-SA 2168-0450 Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2016 ftoslouniv https://doi.org/10.3732/apps.1500036 2020-06-21T08:49:27Z Premise of the study: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). Methods and Results: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosity per locus ranged from 0.06 to 0.68. Analysis of F0 and F1 samples proved that one allele was always inherited maternally. Four multiplex mixes have been developed. Conclusions: Microsatellite markers for this species will be a valuable tool to study detailed small-scale genetic patterns in an arctic/alpine herb and to relate them to demographic parameters. Article in Journal/Newspaper Arctic Silene acaulis Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Arctic Applications in Plant Sciences 3 9 1500036 |
institution |
Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
Premise of the study: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). Methods and Results: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosity per locus ranged from 0.06 to 0.68. Analysis of F0 and F1 samples proved that one allele was always inherited maternally. Four multiplex mixes have been developed. Conclusions: Microsatellite markers for this species will be a valuable tool to study detailed small-scale genetic patterns in an arctic/alpine herb and to relate them to demographic parameters. |
format |
Article in Journal/Newspaper |
author |
Müller, Eike Hlavackova, Iva Svoen, Mildrid Elvik Alsos, Inger Greve Eidesen, Pernille Bronken |
spellingShingle |
Müller, Eike Hlavackova, Iva Svoen, Mildrid Elvik Alsos, Inger Greve Eidesen, Pernille Bronken Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae) |
author_facet |
Müller, Eike Hlavackova, Iva Svoen, Mildrid Elvik Alsos, Inger Greve Eidesen, Pernille Bronken |
author_sort |
Müller, Eike |
title |
Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae) |
title_short |
Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae) |
title_full |
Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae) |
title_fullStr |
Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae) |
title_full_unstemmed |
Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae) |
title_sort |
characterization of 14 microsatellite markers for silene acaulis (caryophyllaceae) |
publisher |
Botanical Society of America |
publishDate |
2016 |
url |
http://hdl.handle.net/10852/49827 http://urn.nb.no/URN:NBN:no-53546 https://doi.org/10.3732/apps.1500036 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Silene acaulis |
genre_facet |
Arctic Silene acaulis |
op_source |
2168-0450 |
op_relation |
http://urn.nb.no/URN:NBN:no-53546 Müller, Eike Hlavackova, Iva Svoen, Mildrid Elvik Alsos, Inger Greve Eidesen, Pernille Bronken . Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae). Applications in Plant Sciences. 2015, 3(9), 1-4 http://hdl.handle.net/10852/49827 1306473 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Applications in Plant Sciences&rft.volume=3&rft.spage=1&rft.date=2015 Applications in Plant Sciences 3 9 1 4 http://dx.doi.org/10.3732/apps.1500036 URN:NBN:no-53546 Fulltext https://www.duo.uio.no/bitstream/handle/10852/49827/1/1306473.pdf |
op_rights |
Attribution-NonCommercial-ShareAlike 3.0 Unported http://creativecommons.org/licenses/by-nc-sa/3.0/ |
op_rightsnorm |
CC-BY-NC-SA |
op_doi |
https://doi.org/10.3732/apps.1500036 |
container_title |
Applications in Plant Sciences |
container_volume |
3 |
container_issue |
9 |
container_start_page |
1500036 |
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1766322588133359616 |