Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures
Background In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology...
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ftoslouniv:oai:www.duo.uio.no:10852/46728 2023-05-15T18:28:25+02:00 Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures Soininen, Eeva M Valentini, Alice Coissac, Eric Miquel, Christian Gielly, Ludovic Brochmann, Christian Brysting, Anne K Sønstebø, Jørn H Ims, Rolf A Yoccoz, Nigel G Taberlet, Pierre 2009 http://hdl.handle.net/10852/46728 http://urn.nb.no/URN:NBN:no-50926 https://doi.org/10.1186/1742-9994-6-16 eng eng http://urn.nb.no/URN:NBN:no-50926 Frontiers in Zoology. 2009 Aug 20;6(1):16 http://hdl.handle.net/10852/46728 http://dx.doi.org/10.1186/1742-9994-6-16 URN:NBN:no-50926 Fulltext https://www.duo.uio.no/bitstream/handle/10852/46728/1/12983_2009_Article_104.pdf Soininen et al; licensee BioMed Central Ltd. Attribution 2.0 Generic http://creativecommons.org/licenses/by/2.0/ CC-BY Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2009 ftoslouniv https://doi.org/10.1186/1742-9994-6-16 2020-06-21T08:48:54Z Background In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus, with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trnL (UAA) intron. Results Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. Conclusion We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores. Article in Journal/Newspaper Subarctic Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Frontiers in Zoology 6 1 16 |
institution |
Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
Background In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus, with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trnL (UAA) intron. Results Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. Conclusion We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores. |
format |
Article in Journal/Newspaper |
author |
Soininen, Eeva M Valentini, Alice Coissac, Eric Miquel, Christian Gielly, Ludovic Brochmann, Christian Brysting, Anne K Sønstebø, Jørn H Ims, Rolf A Yoccoz, Nigel G Taberlet, Pierre |
spellingShingle |
Soininen, Eeva M Valentini, Alice Coissac, Eric Miquel, Christian Gielly, Ludovic Brochmann, Christian Brysting, Anne K Sønstebø, Jørn H Ims, Rolf A Yoccoz, Nigel G Taberlet, Pierre Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
author_facet |
Soininen, Eeva M Valentini, Alice Coissac, Eric Miquel, Christian Gielly, Ludovic Brochmann, Christian Brysting, Anne K Sønstebø, Jørn H Ims, Rolf A Yoccoz, Nigel G Taberlet, Pierre |
author_sort |
Soininen, Eeva M |
title |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_short |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_full |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_fullStr |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_full_unstemmed |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_sort |
analysing diet of small herbivores: the efficiency of dna barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
publishDate |
2009 |
url |
http://hdl.handle.net/10852/46728 http://urn.nb.no/URN:NBN:no-50926 https://doi.org/10.1186/1742-9994-6-16 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
http://urn.nb.no/URN:NBN:no-50926 Frontiers in Zoology. 2009 Aug 20;6(1):16 http://hdl.handle.net/10852/46728 http://dx.doi.org/10.1186/1742-9994-6-16 URN:NBN:no-50926 Fulltext https://www.duo.uio.no/bitstream/handle/10852/46728/1/12983_2009_Article_104.pdf |
op_rights |
Soininen et al; licensee BioMed Central Ltd. Attribution 2.0 Generic http://creativecommons.org/licenses/by/2.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/1742-9994-6-16 |
container_title |
Frontiers in Zoology |
container_volume |
6 |
container_issue |
1 |
container_start_page |
16 |
_version_ |
1766210893491732480 |