Genome and cell size responses to temperature in ectotherms
The relationship between temperature and body size has attracted wide interest since the “Bergmann's rule” was introduced. While this rule originally dealt with endotherms, later studies have focussed ectotherms, including cell- and genome sizes. Because the final body size of an organism is la...
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ftoslouniv:oai:www.duo.uio.no:10852/38101 2024-09-15T17:52:00+00:00 Genome and cell size responses to temperature in ectotherms Johansen, Maria 2013 http://hdl.handle.net/10852/38101 http://urn.nb.no/URN:NBN:no-40492 en eng I. Johansen, M., Wojewodzic, M.W., Laane, C.M.M., and Hessen, D.O. Larger Daphnia at lower temperature; A role for cell size and genome configuration? Genome, 2013 Sep; 56(9): 511-9. Epub 2013 Jun 5. The paper is removed from the thesis in DUO due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1139/gen-2013-0004 II. Johansen, M., Shala, N., Wojewodzic, M.W., Andersen, T., and Hessen, D.O. Multigenerational genomic responses to dietary P and temperature in Daphnia Submitted to Genome, 2013. The paper is removed from the thesis in DUO. III. Leinaas, H.P., Johansen, M., Gabrielsen, T., and Hessen, D.O. Inter- and intra-specific variation in body- and genome size in calanoid copepods; temperature as a key driver? Manuscript. The paper is removed from the thesis in DUO. IV. Johansen, M. and Hessen, D.O. Links between genome, cell, and body size in Drosophila melanogaster raised at different temperatures. Submitted to Journal of Thermal Biology, 2013. The paper is removed from the thesis in DUO. V. Hessen, D.O., Johansen, M., and Svenning, M.A. Erythrocyte size and genome size in Arctic charr (Salvelinus alpinus): A linkage to temperature and body size. Manuscript. The paper is removed from the thesis in DUO. http://dx.doi.org/10.1139/gen-2013-0004 http://urn.nb.no/URN:NBN:no-40492 http://hdl.handle.net/10852/38101 URN:NBN:no-40492 Fulltext https://www.duo.uio.no/bitstream/handle/10852/38101/1/dravhandling-jalal.pdf Doctoral thesis Doktoravhandling 2013 ftoslouniv https://doi.org/10.1139/gen-2013-0004 2024-08-05T14:09:29Z The relationship between temperature and body size has attracted wide interest since the “Bergmann's rule” was introduced. While this rule originally dealt with endotherms, later studies have focussed ectotherms, including cell- and genome sizes. Because the final body size of an organism is largely the sum of its cells, any increase in cell size would lead to an overall increase in body size. For many ectotherms, the negative correlation between body size and temperature is also reflected in a corresponding relationship between temperature and cell- or genome size. For example changes in body size of ectothermic metazoans may partly reflect changes in cell size rather than cell number. While changes in genome size is generally expected to occur over longer time period (evolutionary), except for the case of polyploidization, changes in cell size (cytoplasmic volume) could occur at shorter time scales. For example the responses reflecting geographical (temperatures) clines may differ from those that occur during ontogeny. The main aim of this study was to test whether temperature could affect genome- and cell size in selected ectotherms. The experiments were performed on the following taxa and species; Daphnia (papers I and II), calanoid copepods (paper III), Drosophila melanogaster (paper IV), and Arctic charr (Salvelinus alpinus) (paper V). Genome and cell (nucleus) size showed that the strongest temperature responses were in Daphnia (papers I and II) compared with the other species. Increased body size of Daphnia at low temperatures could, at least partly, be caused by an increase in both DNA condensation and increased cell volume at low temperature (paper I). Our genome size estimates of Daphnia clones (papers I and II), some calanoids (paper III), and Drosophila (embryo and Schnider 2 cells; paper IV) are novel findings. In addition to the temperature effect, we also tested dietary stoichiometric effect on the genome and cell size of Daphnia, by growing it in phosphorus (P) limited versus P complete diet for ... Doctoral or Postdoctoral Thesis Arctic Arctic charr Salvelinus alpinus Copepods Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Genome 56 9 511 519 |
institution |
Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
op_collection_id |
ftoslouniv |
language |
English |
description |
The relationship between temperature and body size has attracted wide interest since the “Bergmann's rule” was introduced. While this rule originally dealt with endotherms, later studies have focussed ectotherms, including cell- and genome sizes. Because the final body size of an organism is largely the sum of its cells, any increase in cell size would lead to an overall increase in body size. For many ectotherms, the negative correlation between body size and temperature is also reflected in a corresponding relationship between temperature and cell- or genome size. For example changes in body size of ectothermic metazoans may partly reflect changes in cell size rather than cell number. While changes in genome size is generally expected to occur over longer time period (evolutionary), except for the case of polyploidization, changes in cell size (cytoplasmic volume) could occur at shorter time scales. For example the responses reflecting geographical (temperatures) clines may differ from those that occur during ontogeny. The main aim of this study was to test whether temperature could affect genome- and cell size in selected ectotherms. The experiments were performed on the following taxa and species; Daphnia (papers I and II), calanoid copepods (paper III), Drosophila melanogaster (paper IV), and Arctic charr (Salvelinus alpinus) (paper V). Genome and cell (nucleus) size showed that the strongest temperature responses were in Daphnia (papers I and II) compared with the other species. Increased body size of Daphnia at low temperatures could, at least partly, be caused by an increase in both DNA condensation and increased cell volume at low temperature (paper I). Our genome size estimates of Daphnia clones (papers I and II), some calanoids (paper III), and Drosophila (embryo and Schnider 2 cells; paper IV) are novel findings. In addition to the temperature effect, we also tested dietary stoichiometric effect on the genome and cell size of Daphnia, by growing it in phosphorus (P) limited versus P complete diet for ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Johansen, Maria |
spellingShingle |
Johansen, Maria Genome and cell size responses to temperature in ectotherms |
author_facet |
Johansen, Maria |
author_sort |
Johansen, Maria |
title |
Genome and cell size responses to temperature in ectotherms |
title_short |
Genome and cell size responses to temperature in ectotherms |
title_full |
Genome and cell size responses to temperature in ectotherms |
title_fullStr |
Genome and cell size responses to temperature in ectotherms |
title_full_unstemmed |
Genome and cell size responses to temperature in ectotherms |
title_sort |
genome and cell size responses to temperature in ectotherms |
publishDate |
2013 |
url |
http://hdl.handle.net/10852/38101 http://urn.nb.no/URN:NBN:no-40492 |
genre |
Arctic Arctic charr Salvelinus alpinus Copepods |
genre_facet |
Arctic Arctic charr Salvelinus alpinus Copepods |
op_relation |
I. Johansen, M., Wojewodzic, M.W., Laane, C.M.M., and Hessen, D.O. Larger Daphnia at lower temperature; A role for cell size and genome configuration? Genome, 2013 Sep; 56(9): 511-9. Epub 2013 Jun 5. The paper is removed from the thesis in DUO due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1139/gen-2013-0004 II. Johansen, M., Shala, N., Wojewodzic, M.W., Andersen, T., and Hessen, D.O. Multigenerational genomic responses to dietary P and temperature in Daphnia Submitted to Genome, 2013. The paper is removed from the thesis in DUO. III. Leinaas, H.P., Johansen, M., Gabrielsen, T., and Hessen, D.O. Inter- and intra-specific variation in body- and genome size in calanoid copepods; temperature as a key driver? Manuscript. The paper is removed from the thesis in DUO. IV. Johansen, M. and Hessen, D.O. Links between genome, cell, and body size in Drosophila melanogaster raised at different temperatures. Submitted to Journal of Thermal Biology, 2013. The paper is removed from the thesis in DUO. V. Hessen, D.O., Johansen, M., and Svenning, M.A. Erythrocyte size and genome size in Arctic charr (Salvelinus alpinus): A linkage to temperature and body size. Manuscript. The paper is removed from the thesis in DUO. http://dx.doi.org/10.1139/gen-2013-0004 http://urn.nb.no/URN:NBN:no-40492 http://hdl.handle.net/10852/38101 URN:NBN:no-40492 Fulltext https://www.duo.uio.no/bitstream/handle/10852/38101/1/dravhandling-jalal.pdf |
op_doi |
https://doi.org/10.1139/gen-2013-0004 |
container_title |
Genome |
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56 |
container_issue |
9 |
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511 |
op_container_end_page |
519 |
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1810294075610365952 |