Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod
Lipid metabolism is essential in maintaining energy homeostasis in multicellular organisms. In vertebrates, the peroxisome proliferator-activated receptors (PPARs, NR1C) regulate the expression of many genes involved in these processes. Atlantic cod ( Gadus morhua ) is an important fish species in t...
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ftoslouniv:oai:www.duo.uio.no:10852/105777 2023-12-10T09:46:30+01:00 Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod ENEngelskEnglishIntegrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod Eide, Marta Goksøyr, Anders Yadetie, Fekadu Gilabert, Alejandra Bartosova, Zdenka Frøysa, Håvard Guldbrandsen Fallahi, Shirin Zhang, Xiaokang Blaser, Nello Jonassen, Inge Bruheim, Per Alendal, Guttorm Brun, Morten Porte, Cinta Karlsen, Odd André 2023-04-28T11:29:29Z http://hdl.handle.net/10852/105777 https://doi.org/10.3389/fphys.2023.1129089 EN eng Eide, Marta Goksøyr, Anders Yadetie, Fekadu Gilabert, Alejandra Bartosova, Zdenka Frøysa, Håvard Guldbrandsen Fallahi, Shirin Zhang, Xiaokang Blaser, Nello Jonassen, Inge Bruheim, Per Alendal, Guttorm Brun, Morten Porte, Cinta Karlsen, Odd André . Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod. Frontiers in Physiology. 2023, 14 http://hdl.handle.net/10852/105777 2144156 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers in Physiology&rft.volume=14&rft.spage=&rft.date=2023 Frontiers in Physiology 14 0 https://doi.org/10.3389/fphys.2023.1129089 Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ 1664-042X Journal article Tidsskriftartikkel Peer reviewed PublishedVersion 2023 ftoslouniv https://doi.org/10.3389/fphys.2023.1129089 2023-11-15T23:39:43Z Lipid metabolism is essential in maintaining energy homeostasis in multicellular organisms. In vertebrates, the peroxisome proliferator-activated receptors (PPARs, NR1C) regulate the expression of many genes involved in these processes. Atlantic cod ( Gadus morhua ) is an important fish species in the North Atlantic ecosystem and in human nutrition, with a highly fatty liver. Here we study the involvement of Atlantic cod Ppar a and b subtypes in systemic regulation of lipid metabolism using two model agonists after in vivo exposure. WY-14,643, a specific PPARA ligand in mammals, activated cod Ppara1 and Ppara2 in vitro . In vivo , WY-14,643 caused a shift in lipid transport both at transcriptional and translational level in cod. However, WY-14,643 induced fewer genes in the fatty acid beta-oxidation pathway compared to that observed in rodents. Although GW501516 serves as a specific PPARB/D ligand in mammals, this compound activated cod Ppara1 and Ppara2 as well as Pparb in vitro . In vivo , it further induced transcription of Ppar target genes and caused changes in lipid composition of liver and plasma. The integrative approach provide a foundation for understanding how Ppars are engaged in regulating lipid metabolism in Atlantic cod physiology. We have shown that WY-14,643 and GW501516 activate Atlantic cod Ppara and Pparb, affect genes in lipid metabolism pathways, and induce changes in the lipid composition in plasma and liver microsomal membranes. Particularly, the combined transcriptomic, proteomics and lipidomics analyses revealed that effects of WY-14,643 on lipid metabolism are similar to what is known in mammalian studies, suggesting conservation of Ppara functions in mediating lipid metabolic processes in fish. The alterations in the lipid profiles observed after Ppar agonist exposure suggest that other chemicals with similar Ppar receptor affinities may cause disturbances in the lipid regulation of fish. Model organism: Atlantic cod ( Gadus morhua ). LSID: ... Article in Journal/Newspaper atlantic cod Gadus morhua North Atlantic Universitet i Oslo: Digitale utgivelser ved UiO (DUO) Frontiers in Physiology 14 |
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Open Polar |
collection |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
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ftoslouniv |
language |
English |
description |
Lipid metabolism is essential in maintaining energy homeostasis in multicellular organisms. In vertebrates, the peroxisome proliferator-activated receptors (PPARs, NR1C) regulate the expression of many genes involved in these processes. Atlantic cod ( Gadus morhua ) is an important fish species in the North Atlantic ecosystem and in human nutrition, with a highly fatty liver. Here we study the involvement of Atlantic cod Ppar a and b subtypes in systemic regulation of lipid metabolism using two model agonists after in vivo exposure. WY-14,643, a specific PPARA ligand in mammals, activated cod Ppara1 and Ppara2 in vitro . In vivo , WY-14,643 caused a shift in lipid transport both at transcriptional and translational level in cod. However, WY-14,643 induced fewer genes in the fatty acid beta-oxidation pathway compared to that observed in rodents. Although GW501516 serves as a specific PPARB/D ligand in mammals, this compound activated cod Ppara1 and Ppara2 as well as Pparb in vitro . In vivo , it further induced transcription of Ppar target genes and caused changes in lipid composition of liver and plasma. The integrative approach provide a foundation for understanding how Ppars are engaged in regulating lipid metabolism in Atlantic cod physiology. We have shown that WY-14,643 and GW501516 activate Atlantic cod Ppara and Pparb, affect genes in lipid metabolism pathways, and induce changes in the lipid composition in plasma and liver microsomal membranes. Particularly, the combined transcriptomic, proteomics and lipidomics analyses revealed that effects of WY-14,643 on lipid metabolism are similar to what is known in mammalian studies, suggesting conservation of Ppara functions in mediating lipid metabolic processes in fish. The alterations in the lipid profiles observed after Ppar agonist exposure suggest that other chemicals with similar Ppar receptor affinities may cause disturbances in the lipid regulation of fish. Model organism: Atlantic cod ( Gadus morhua ). LSID: ... |
format |
Article in Journal/Newspaper |
author |
Eide, Marta Goksøyr, Anders Yadetie, Fekadu Gilabert, Alejandra Bartosova, Zdenka Frøysa, Håvard Guldbrandsen Fallahi, Shirin Zhang, Xiaokang Blaser, Nello Jonassen, Inge Bruheim, Per Alendal, Guttorm Brun, Morten Porte, Cinta Karlsen, Odd André |
spellingShingle |
Eide, Marta Goksøyr, Anders Yadetie, Fekadu Gilabert, Alejandra Bartosova, Zdenka Frøysa, Håvard Guldbrandsen Fallahi, Shirin Zhang, Xiaokang Blaser, Nello Jonassen, Inge Bruheim, Per Alendal, Guttorm Brun, Morten Porte, Cinta Karlsen, Odd André Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
author_facet |
Eide, Marta Goksøyr, Anders Yadetie, Fekadu Gilabert, Alejandra Bartosova, Zdenka Frøysa, Håvard Guldbrandsen Fallahi, Shirin Zhang, Xiaokang Blaser, Nello Jonassen, Inge Bruheim, Per Alendal, Guttorm Brun, Morten Porte, Cinta Karlsen, Odd André |
author_sort |
Eide, Marta |
title |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_short |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_full |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_fullStr |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_full_unstemmed |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_sort |
integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male atlantic cod |
publishDate |
2023 |
url |
http://hdl.handle.net/10852/105777 https://doi.org/10.3389/fphys.2023.1129089 |
genre |
atlantic cod Gadus morhua North Atlantic |
genre_facet |
atlantic cod Gadus morhua North Atlantic |
op_source |
1664-042X |
op_relation |
Eide, Marta Goksøyr, Anders Yadetie, Fekadu Gilabert, Alejandra Bartosova, Zdenka Frøysa, Håvard Guldbrandsen Fallahi, Shirin Zhang, Xiaokang Blaser, Nello Jonassen, Inge Bruheim, Per Alendal, Guttorm Brun, Morten Porte, Cinta Karlsen, Odd André . Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod. Frontiers in Physiology. 2023, 14 http://hdl.handle.net/10852/105777 2144156 info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers in Physiology&rft.volume=14&rft.spage=&rft.date=2023 Frontiers in Physiology 14 0 https://doi.org/10.3389/fphys.2023.1129089 |
op_rights |
Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3389/fphys.2023.1129089 |
container_title |
Frontiers in Physiology |
container_volume |
14 |
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1784889950071685120 |