Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling
Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic microalgal taxa is the dinoflagellate Genus Alexandrium, that includes species p...
Published in: | Aquatic Toxicology |
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Language: | English |
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2018
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Online Access: | https://oskar-bordeaux.fr/handle/20.500.12278/199628 https://hdl.handle.net/20.500.12278/199628 https://doi.org/10.1016/j.aquatox.2018.03.030 |
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/199628 2024-06-16T07:39:32+00:00 Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling MAT, Audrey KLOPP, Christophe PAYTON, Laura JEZIORSKI, Céline CHALOPIN, Morgane AMZIL, Zouher TRAN, Damien WIKFORS, Gary HEGARET, Helene SOUDANT, Philippe HUVET, Arnaud FABIOUX, Caroline 2018-06 https://oskar-bordeaux.fr/handle/20.500.12278/199628 https://hdl.handle.net/20.500.12278/199628 https://doi.org/10.1016/j.aquatox.2018.03.030 EN eng 0166-445X https://oskar-bordeaux.fr/handle/20.500.12278/199628 doi:10.1016/j.aquatox.2018.03.030 open Pas de Licence CC Transcriptomic Ions channels ACL Paralytic shellfish toxins Elastic-net regression Sciences de l'environnement Sciences du Vivant [q-bio] Sciences du Vivant [q-bio]/Ecologie Environnement Sciences de l'environnement/Biodiversité et Ecologie Article de revue 2018 ftoskarbordeaux https://doi.org/20.500.12278/19962810.1016/j.aquatox.2018.03.030 2024-05-19T23:40:38Z Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic microalgal taxa is the dinoflagellate Genus Alexandrium, that includes species producing neurotoxins referred to as PST (Paralytic Shellfish Toxins). Blooms cause shellfish harvest restrictions to protect human consumers from accumulated toxins. Large inter-individual variability in toxin load within an exposed bivalve population complicates monitoring of shellfish toxicity for ecology and human health regulation. To decipher the physiological pathways involved in the bivalve response to PST, we explored the whole transcriptome of the digestive gland of the Pacific oyster Crassostrea gigas fed experimentally with a toxic Alexandrium minutum culture. The largest differences in transcript abundance were between oysters with contrasting toxin loads (1098 transcripts), rather than between exposed and non-exposed oysters (16 transcripts), emphasizing the importance of toxin load in oyster response to toxic dinoflagellates. Additionally, penalized regressions, innovative in this field, modeled accurately toxin load based upon only 70 transcripts. Transcriptomic differences between oysters with contrasting PST burdens revealed a limited suite of metabolic pathways affected, including ion channels, neuromuscular communication, and digestion, all of which are interconnected and linked to sodium and calcium exchanges. Carbohydrate metabolism, unconsidered previously in studies of harmful algal effects on shellfish, was also highlighted, suggesting energy challenge in oysters with high toxin loads. Associations between toxin load, genotype, and mRNA levels were revealed that open new doors for genetic studies identifying genetically-based low toxin accumulation. De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster OSKAR Bordeaux (Open Science Knowledge ARchive) Pacific Aquatic Toxicology 199 127 137 |
institution |
Open Polar |
collection |
OSKAR Bordeaux (Open Science Knowledge ARchive) |
op_collection_id |
ftoskarbordeaux |
language |
English |
topic |
Transcriptomic Ions channels ACL Paralytic shellfish toxins Elastic-net regression Sciences de l'environnement Sciences du Vivant [q-bio] Sciences du Vivant [q-bio]/Ecologie Environnement Sciences de l'environnement/Biodiversité et Ecologie |
spellingShingle |
Transcriptomic Ions channels ACL Paralytic shellfish toxins Elastic-net regression Sciences de l'environnement Sciences du Vivant [q-bio] Sciences du Vivant [q-bio]/Ecologie Environnement Sciences de l'environnement/Biodiversité et Ecologie MAT, Audrey KLOPP, Christophe PAYTON, Laura JEZIORSKI, Céline CHALOPIN, Morgane AMZIL, Zouher TRAN, Damien WIKFORS, Gary HEGARET, Helene SOUDANT, Philippe HUVET, Arnaud FABIOUX, Caroline Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
topic_facet |
Transcriptomic Ions channels ACL Paralytic shellfish toxins Elastic-net regression Sciences de l'environnement Sciences du Vivant [q-bio] Sciences du Vivant [q-bio]/Ecologie Environnement Sciences de l'environnement/Biodiversité et Ecologie |
description |
Harmful Algal Blooms are worldwide occurrences that can cause poisoning in human seafood consumers as well as mortality and sublethal effets in wildlife, propagating economic losses. One of the most widespread toxigenic microalgal taxa is the dinoflagellate Genus Alexandrium, that includes species producing neurotoxins referred to as PST (Paralytic Shellfish Toxins). Blooms cause shellfish harvest restrictions to protect human consumers from accumulated toxins. Large inter-individual variability in toxin load within an exposed bivalve population complicates monitoring of shellfish toxicity for ecology and human health regulation. To decipher the physiological pathways involved in the bivalve response to PST, we explored the whole transcriptome of the digestive gland of the Pacific oyster Crassostrea gigas fed experimentally with a toxic Alexandrium minutum culture. The largest differences in transcript abundance were between oysters with contrasting toxin loads (1098 transcripts), rather than between exposed and non-exposed oysters (16 transcripts), emphasizing the importance of toxin load in oyster response to toxic dinoflagellates. Additionally, penalized regressions, innovative in this field, modeled accurately toxin load based upon only 70 transcripts. Transcriptomic differences between oysters with contrasting PST burdens revealed a limited suite of metabolic pathways affected, including ion channels, neuromuscular communication, and digestion, all of which are interconnected and linked to sodium and calcium exchanges. Carbohydrate metabolism, unconsidered previously in studies of harmful algal effects on shellfish, was also highlighted, suggesting energy challenge in oysters with high toxin loads. Associations between toxin load, genotype, and mRNA levels were revealed that open new doors for genetic studies identifying genetically-based low toxin accumulation. De la caractérisation des déterminants de l'accumulation des toxines paralysantes (PST) chez l'huître (Crassostrea gigas) au risque sanitaire ... |
format |
Article in Journal/Newspaper |
author |
MAT, Audrey KLOPP, Christophe PAYTON, Laura JEZIORSKI, Céline CHALOPIN, Morgane AMZIL, Zouher TRAN, Damien WIKFORS, Gary HEGARET, Helene SOUDANT, Philippe HUVET, Arnaud FABIOUX, Caroline |
author_facet |
MAT, Audrey KLOPP, Christophe PAYTON, Laura JEZIORSKI, Céline CHALOPIN, Morgane AMZIL, Zouher TRAN, Damien WIKFORS, Gary HEGARET, Helene SOUDANT, Philippe HUVET, Arnaud FABIOUX, Caroline |
author_sort |
MAT, Audrey |
title |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_short |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_full |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_fullStr |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_full_unstemmed |
Oyster transcriptome response to Alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
title_sort |
oyster transcriptome response to alexandrium exposure is related to saxitoxin load and characterized by disrupted digestion, energy balance, and calcium and sodium signaling |
publishDate |
2018 |
url |
https://oskar-bordeaux.fr/handle/20.500.12278/199628 https://hdl.handle.net/20.500.12278/199628 https://doi.org/10.1016/j.aquatox.2018.03.030 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
0166-445X https://oskar-bordeaux.fr/handle/20.500.12278/199628 doi:10.1016/j.aquatox.2018.03.030 |
op_rights |
open Pas de Licence CC |
op_doi |
https://doi.org/20.500.12278/19962810.1016/j.aquatox.2018.03.030 |
container_title |
Aquatic Toxicology |
container_volume |
199 |
container_start_page |
127 |
op_container_end_page |
137 |
_version_ |
1802006296812060672 |