Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves
Inhibition of the mitochondrial electron transfer chain and induction of reactive oxygen species (ROS) production are one of the roots of cadmium (Cd) toxicity. To appreciate the impact of Cd on mitochondria, we focused on the expression of CoxI gene which encodes the subunit I of the Cytochrome c o...
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/199014 2024-05-19T07:39:18+00:00 Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves ACHARD-JORIS, Maud GONZALEZ, Patrice MARIE, Véronique BAUDRIMONT, Magalie BOURDINEAUD, Jean-Paul 2006-06-01 https://oskar-bordeaux.fr/handle/20.500.12278/199014 https://hdl.handle.net/20.500.12278/199014 https://www.researchgate.net/publication/6984395_Cytochrome_c_Oxydase_Subunit_I_Gene_is_Up-regulated_by_Cadmium_in_Freshwater_and_Marine_Bivalves https://doi.org/10.1007/s10534-005-5671-9 EN eng 0966-0844 https://www.researchgate.net/publication/6984395_Cytochrome_c_Oxydase_Subunit_I_Gene_is_Up-regulated_by_Cadmium_in_Freshwater_and_Marine_Bivalves oai:crossref.org:10.1007/s10534-005-5671-9 oai:researchgate.net:6984395 https://oskar-bordeaux.fr/handle/20.500.12278/199014 doi:10.1007/s10534-005-5671-9 open Pas de Licence CC crossref researchgate Sciences de l'environnement Article de revue 2006 ftoskarbordeaux https://doi.org/20.500.12278/19901410.1007/s10534-005-5671-9 2024-04-22T01:37:53Z Inhibition of the mitochondrial electron transfer chain and induction of reactive oxygen species (ROS) production are one of the roots of cadmium (Cd) toxicity. To appreciate the impact of Cd on mitochondria, we focused on the expression of CoxI gene which encodes the subunit I of the Cytochrome c oxidase (complex IV of the respiratory chain). CoxI gene expression was studied by real-time quantitative PCR in three species: two freshwater bivalves (Corbicula fluminea and Dreissena polymorpha) and one marine bivalve (diploid or triploid Crassostrea gigas). Bivalves were exposed for 10 or 14 days to 0.13 microM Cd(2+) and 15.3 microM Zn(2+) in controlled laboratory conditions. We demonstrate that in the three mollusk species CoxI gene was up-regulated by Cd. Zinc (Zn), which is known to have antioxidant properties, had no effect on CoxI gene expression. In the presence of Cd and Zn, CoxI gene inducibility was lower than after a single Cd exposure, in each species; result that could not be fully explained by a decreased Cd accumulation. CoxI gene induction by Cd was 4.8-fold higher in triploid oysters than in diploid ones, indicating a possible influence of triploidy on animal responses to Cd contamination. Article in Journal/Newspaper Crassostrea gigas OSKAR Bordeaux (Open Science Knowledge ARchive) BioMetals 19 3 237 244 |
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OSKAR Bordeaux (Open Science Knowledge ARchive) |
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ftoskarbordeaux |
language |
English |
topic |
Sciences de l'environnement |
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Sciences de l'environnement ACHARD-JORIS, Maud GONZALEZ, Patrice MARIE, Véronique BAUDRIMONT, Magalie BOURDINEAUD, Jean-Paul Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves |
topic_facet |
Sciences de l'environnement |
description |
Inhibition of the mitochondrial electron transfer chain and induction of reactive oxygen species (ROS) production are one of the roots of cadmium (Cd) toxicity. To appreciate the impact of Cd on mitochondria, we focused on the expression of CoxI gene which encodes the subunit I of the Cytochrome c oxidase (complex IV of the respiratory chain). CoxI gene expression was studied by real-time quantitative PCR in three species: two freshwater bivalves (Corbicula fluminea and Dreissena polymorpha) and one marine bivalve (diploid or triploid Crassostrea gigas). Bivalves were exposed for 10 or 14 days to 0.13 microM Cd(2+) and 15.3 microM Zn(2+) in controlled laboratory conditions. We demonstrate that in the three mollusk species CoxI gene was up-regulated by Cd. Zinc (Zn), which is known to have antioxidant properties, had no effect on CoxI gene expression. In the presence of Cd and Zn, CoxI gene inducibility was lower than after a single Cd exposure, in each species; result that could not be fully explained by a decreased Cd accumulation. CoxI gene induction by Cd was 4.8-fold higher in triploid oysters than in diploid ones, indicating a possible influence of triploidy on animal responses to Cd contamination. |
format |
Article in Journal/Newspaper |
author |
ACHARD-JORIS, Maud GONZALEZ, Patrice MARIE, Véronique BAUDRIMONT, Magalie BOURDINEAUD, Jean-Paul |
author_facet |
ACHARD-JORIS, Maud GONZALEZ, Patrice MARIE, Véronique BAUDRIMONT, Magalie BOURDINEAUD, Jean-Paul |
author_sort |
ACHARD-JORIS, Maud |
title |
Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves |
title_short |
Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves |
title_full |
Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves |
title_fullStr |
Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves |
title_full_unstemmed |
Cytochrome c Oxydase Subunit I Gene is Up-regulated by Cadmium in Freshwater and Marine Bivalves |
title_sort |
cytochrome c oxydase subunit i gene is up-regulated by cadmium in freshwater and marine bivalves |
publishDate |
2006 |
url |
https://oskar-bordeaux.fr/handle/20.500.12278/199014 https://hdl.handle.net/20.500.12278/199014 https://www.researchgate.net/publication/6984395_Cytochrome_c_Oxydase_Subunit_I_Gene_is_Up-regulated_by_Cadmium_in_Freshwater_and_Marine_Bivalves https://doi.org/10.1007/s10534-005-5671-9 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
crossref researchgate |
op_relation |
0966-0844 https://www.researchgate.net/publication/6984395_Cytochrome_c_Oxydase_Subunit_I_Gene_is_Up-regulated_by_Cadmium_in_Freshwater_and_Marine_Bivalves oai:crossref.org:10.1007/s10534-005-5671-9 oai:researchgate.net:6984395 https://oskar-bordeaux.fr/handle/20.500.12278/199014 doi:10.1007/s10534-005-5671-9 |
op_rights |
open Pas de Licence CC |
op_doi |
https://doi.org/20.500.12278/19901410.1007/s10534-005-5671-9 |
container_title |
BioMetals |
container_volume |
19 |
container_issue |
3 |
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237 |
op_container_end_page |
244 |
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1799478866624905216 |