An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas
Frequent occurrences of pesticides in the environment have raised concerns that combined exposure to these chemicals may result in enhanced toxicity through additive or synergistic interaction between compounds. Spermatozoa and embryos of the Pacific oyster, Crassostrea gigas, were exposed to differ...
Published in: | Environmental Science and Pollution Research |
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Online Access: | https://oskar-bordeaux.fr/handle/20.500.12278/187312 https://hdl.handle.net/20.500.12278/187312 https://doi.org/10.1007/s11356-018-3586-6 |
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/187312 2024-02-11T10:03:07+01:00 An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas MAI, Huong CACHOT, Jerome CLÉRANDEAU, Christelle CAROLINE, Martin MAZZELLA, Nicolas GONZALEZ, Patrice MORIN, Bénédicte 2020-02 https://oskar-bordeaux.fr/handle/20.500.12278/187312 https://hdl.handle.net/20.500.12278/187312 https://doi.org/10.1007/s11356-018-3586-6 EN eng 0944-1344 https://oskar-bordeaux.fr/handle/20.500.12278/187312 doi:10.1007/s11356-018-3586-6 open Pas de Licence CC Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster Sciences de l'environnement Article de revue 2020 ftoskarbordeaux https://doi.org/20.500.12278/18731210.1007/s11356-018-3586-6 2024-01-23T23:30:47Z Frequent occurrences of pesticides in the environment have raised concerns that combined exposure to these chemicals may result in enhanced toxicity through additive or synergistic interaction between compounds. Spermatozoa and embryos of the Pacific oyster, Crassostrea gigas, were exposed to different concentrations of a pesticide mixture with and without copper, mimicking the cocktail of pollutants occurring in the oyster culture area of Arcachon Bay. For the 1× exposure condition, measured concentration corresponds to a total concentration of 1.083 μg L−1 for the mixture of 14 pesticides and to 6.330 μg L−1 for copper (Cu). Several endpoints including larval abnormalities, DNA damage to spermatozoa and embryo and gene expression in D-larvae were investigated. Results demonstrated that pesticide mixtures in combination with or without copper induced a dose-dependent increase in embryotoxic and genotoxic effects on D-larvae from the lowest tested dose of 0.1×. Transcription of genes involved in anti-oxidative stress (cat), respiratory chain (coxI), metal detoxification (mt1 and mt2), and cell cycle arrest and apoptosis (p53) was found to be significantly downregulated while the xenobiotic biotransformation gene gst was significantly upregulated in embryos exposed to pesticide mixture with and without Cu. These findings raise the question of the possible impacts of mixtures of pesticides and metals on wild or farmed oyster populations from polluted coastal marine areas. Article in Journal/Newspaper Crassostrea gigas Pacific oyster OSKAR Bordeaux (Open Science Knowledge ARchive) Pacific Environmental Science and Pollution Research 27 4 3600 3611 |
institution |
Open Polar |
collection |
OSKAR Bordeaux (Open Science Knowledge ARchive) |
op_collection_id |
ftoskarbordeaux |
language |
English |
topic |
Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster Sciences de l'environnement |
spellingShingle |
Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster Sciences de l'environnement MAI, Huong CACHOT, Jerome CLÉRANDEAU, Christelle CAROLINE, Martin MAZZELLA, Nicolas GONZALEZ, Patrice MORIN, Bénédicte An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
topic_facet |
Pesticide mixture Copper Gene expression Genotoxicity Embryotoxicity Pacific oyster Sciences de l'environnement |
description |
Frequent occurrences of pesticides in the environment have raised concerns that combined exposure to these chemicals may result in enhanced toxicity through additive or synergistic interaction between compounds. Spermatozoa and embryos of the Pacific oyster, Crassostrea gigas, were exposed to different concentrations of a pesticide mixture with and without copper, mimicking the cocktail of pollutants occurring in the oyster culture area of Arcachon Bay. For the 1× exposure condition, measured concentration corresponds to a total concentration of 1.083 μg L−1 for the mixture of 14 pesticides and to 6.330 μg L−1 for copper (Cu). Several endpoints including larval abnormalities, DNA damage to spermatozoa and embryo and gene expression in D-larvae were investigated. Results demonstrated that pesticide mixtures in combination with or without copper induced a dose-dependent increase in embryotoxic and genotoxic effects on D-larvae from the lowest tested dose of 0.1×. Transcription of genes involved in anti-oxidative stress (cat), respiratory chain (coxI), metal detoxification (mt1 and mt2), and cell cycle arrest and apoptosis (p53) was found to be significantly downregulated while the xenobiotic biotransformation gene gst was significantly upregulated in embryos exposed to pesticide mixture with and without Cu. These findings raise the question of the possible impacts of mixtures of pesticides and metals on wild or farmed oyster populations from polluted coastal marine areas. |
format |
Article in Journal/Newspaper |
author |
MAI, Huong CACHOT, Jerome CLÉRANDEAU, Christelle CAROLINE, Martin MAZZELLA, Nicolas GONZALEZ, Patrice MORIN, Bénédicte |
author_facet |
MAI, Huong CACHOT, Jerome CLÉRANDEAU, Christelle CAROLINE, Martin MAZZELLA, Nicolas GONZALEZ, Patrice MORIN, Bénédicte |
author_sort |
MAI, Huong |
title |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_short |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_full |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_fullStr |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas |
title_sort |
environmentally realistic pesticide and copper mixture impacts embryonic development and dna integrity of the pacific oyster, crassostrea gigas |
publishDate |
2020 |
url |
https://oskar-bordeaux.fr/handle/20.500.12278/187312 https://hdl.handle.net/20.500.12278/187312 https://doi.org/10.1007/s11356-018-3586-6 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
0944-1344 https://oskar-bordeaux.fr/handle/20.500.12278/187312 doi:10.1007/s11356-018-3586-6 |
op_rights |
open Pas de Licence CC |
op_doi |
https://doi.org/20.500.12278/18731210.1007/s11356-018-3586-6 |
container_title |
Environmental Science and Pollution Research |
container_volume |
27 |
container_issue |
4 |
container_start_page |
3600 |
op_container_end_page |
3611 |
_version_ |
1790599280436707328 |