The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia
A mercury-resistant bacterial strain has been isolated from a rock of the Idrija mercury mine in Slovenia. The rock had 19 g carbon and 2952 mg mercury (Hg) per kg. Mass spectrometry and DNA sequencing showed that the bacterium belongs to the Pseudomonas genus. It is called Pseudomonas idrijaensis....
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/182400 2023-06-18T03:42:40+02:00 The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia BOURDINEAUD, Jean-Paul DURN, Goran REŽUN, Bojan MANCEAU, Alain HRENOVIĆ, Jasna 2020 https://oskar-bordeaux.fr/handle/20.500.12278/182400 https://hdl.handle.net/20.500.12278/182400 https://doi.org/10.1016/j.chemosphere.2020.126002 EN eng 0045-6535 https://oskar-bordeaux.fr/handle/20.500.12278/182400 doi:10.1016/j.chemosphere.2020.126002 open Pas de Licence CC Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie Sciences de l'environnement Article de revue 2020 ftoskarbordeaux https://doi.org/20.500.12278/18240010.1016/j.chemosphere.2020.126002 2023-06-06T22:31:40Z A mercury-resistant bacterial strain has been isolated from a rock of the Idrija mercury mine in Slovenia. The rock had 19 g carbon and 2952 mg mercury (Hg) per kg. Mass spectrometry and DNA sequencing showed that the bacterium belongs to the Pseudomonas genus. It is called Pseudomonas idrijaensis. This bacterial strain is sensitive to methylmercury (MeHg) like the reference P. aeruginosa strain PAO1, and is resistant to divalent mercury (Hg(II)) in contrast to PAO1. This difference could be attributed to the presence of the mer operon yet deprived of the merB gene encoding the organomercurial lyase, on the basis of whole genome sequencing. The P. idrijaensis mer operon displays the RTPCADE organization and is contained in the Tn5041 transposon. This transposon identified here occurs in other Gram-negative Hg-resistant strains isolated from mercury ores, aquatic systems and soils, including Pseudomonas strains from 15,000 to 40,000 years old Siberian permafrost. When P. idrijaensis was exposed to mercury chloride, two intracellular Hg species were identified by high energy-resolution XANES spectroscopy, a dithiolate Hg(SR)2 and a tetrathiolate Hg(SR)4 complex. P. idrijaensis had a much higher [Hg(SR)2]/[Hg(SR)4] molar ratio than bacteria lacking the mer operon when exposed to 4 µg Hg2+/L - resulting in an intracellular accumulation of 4.3 µg Hg/g dw. A higher amount of the Hg(SR)2 complex provides a chemical signature for the expression of the dicysteinate Mer proteins in response to mercury toxicity. Article in Journal/Newspaper permafrost OSKAR Bordeaux (Open Science Knowledge ARchive) Chemosphere 248 126002 |
institution |
Open Polar |
collection |
OSKAR Bordeaux (Open Science Knowledge ARchive) |
op_collection_id |
ftoskarbordeaux |
language |
English |
topic |
Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie Sciences de l'environnement |
spellingShingle |
Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie Sciences de l'environnement BOURDINEAUD, Jean-Paul DURN, Goran REŽUN, Bojan MANCEAU, Alain HRENOVIĆ, Jasna The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
topic_facet |
Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie Sciences de l'environnement |
description |
A mercury-resistant bacterial strain has been isolated from a rock of the Idrija mercury mine in Slovenia. The rock had 19 g carbon and 2952 mg mercury (Hg) per kg. Mass spectrometry and DNA sequencing showed that the bacterium belongs to the Pseudomonas genus. It is called Pseudomonas idrijaensis. This bacterial strain is sensitive to methylmercury (MeHg) like the reference P. aeruginosa strain PAO1, and is resistant to divalent mercury (Hg(II)) in contrast to PAO1. This difference could be attributed to the presence of the mer operon yet deprived of the merB gene encoding the organomercurial lyase, on the basis of whole genome sequencing. The P. idrijaensis mer operon displays the RTPCADE organization and is contained in the Tn5041 transposon. This transposon identified here occurs in other Gram-negative Hg-resistant strains isolated from mercury ores, aquatic systems and soils, including Pseudomonas strains from 15,000 to 40,000 years old Siberian permafrost. When P. idrijaensis was exposed to mercury chloride, two intracellular Hg species were identified by high energy-resolution XANES spectroscopy, a dithiolate Hg(SR)2 and a tetrathiolate Hg(SR)4 complex. P. idrijaensis had a much higher [Hg(SR)2]/[Hg(SR)4] molar ratio than bacteria lacking the mer operon when exposed to 4 µg Hg2+/L - resulting in an intracellular accumulation of 4.3 µg Hg/g dw. A higher amount of the Hg(SR)2 complex provides a chemical signature for the expression of the dicysteinate Mer proteins in response to mercury toxicity. |
format |
Article in Journal/Newspaper |
author |
BOURDINEAUD, Jean-Paul DURN, Goran REŽUN, Bojan MANCEAU, Alain HRENOVIĆ, Jasna |
author_facet |
BOURDINEAUD, Jean-Paul DURN, Goran REŽUN, Bojan MANCEAU, Alain HRENOVIĆ, Jasna |
author_sort |
BOURDINEAUD, Jean-Paul |
title |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_short |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_full |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_fullStr |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_full_unstemmed |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_sort |
chemical species of mercury accumulated by pseudomonas idrijaensis, a bacterium from a rock of the idrija mercury mine, slovenia |
publishDate |
2020 |
url |
https://oskar-bordeaux.fr/handle/20.500.12278/182400 https://hdl.handle.net/20.500.12278/182400 https://doi.org/10.1016/j.chemosphere.2020.126002 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
0045-6535 https://oskar-bordeaux.fr/handle/20.500.12278/182400 doi:10.1016/j.chemosphere.2020.126002 |
op_rights |
open Pas de Licence CC |
op_doi |
https://doi.org/20.500.12278/18240010.1016/j.chemosphere.2020.126002 |
container_title |
Chemosphere |
container_volume |
248 |
container_start_page |
126002 |
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1769008694961373184 |