New insights into detecting alizarin from autofluorescence in marked glass eels
Alizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologie...
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Online Access: | https://oskar-bordeaux.fr/handle/20.500.12278/170392 https://hdl.handle.net/20.500.12278/170392 https://doi.org/10.1038/s41598-022-18440-0 |
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/170392 2023-05-15T13:27:48+02:00 New insights into detecting alizarin from autofluorescence in marked glass eels GAILLARD, Melanie PARLANTI, Edith SOURZAC, Mahaut COUILLAUD, Franck GENEVOIS, Coralie BOUTRY, Sebastien RIGAUD, Christian DAVERAT, Francoise 2022-09-26 https://oskar-bordeaux.fr/handle/20.500.12278/170392 https://hdl.handle.net/20.500.12278/170392 https://doi.org/10.1038/s41598-022-18440-0 EN eng 2045-2322 https://oskar-bordeaux.fr/handle/20.500.12278/170392 doi:10.1038/s41598-022-18440-0 Attribution 3.0 United States open http://creativecommons.org/licenses/by/3.0/us/ CC BY CC-BY Ecology Imaging Marine biology Microscopy Optical spectroscopy Sciences de l'environnement Article de revue 2022 ftoskarbordeaux https://doi.org/20.500.12278/170392 https://doi.org/10.1038/s41598-022-18440-0 2023-01-10T23:30:42Z Alizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologies on the living body and fins of European glass eels (Anguilla anguilla L.). The findings demonstrated that the control group had a high autofluorescence at alizarin and eelGFP maxima bands. With fluorescence reflectance imaging (FRI), the eel living body autofluorescence impeded the detection of the marked eels. In contrast with experimental excitation-emission-matrix (EEM) fluorescence analyses, 99% of the marked eels were correctly assigned to their group from fluorescence analyses of their fin cellular contents. With epifluorometry (EPI), 100% of the marked eels were detected with the caudal fin tips when excited at 450–490 nm wavelengths due to a weaker autofluorescence signal. EEM and FRI assays unveiled an average fluorescence quenching 60% and 44% of the marked group respectively, in the alizarin and eelGFP maxima bands. The fluorescence quenching observed is discussed. Results will benefit experimental design by examining autofluorescence effects on mark detection and the development of non-invasive detection methods in this critically endangered species. Other/Unknown Material Anguilla anguilla OSKAR Bordeaux (Open Science Knowledge ARchive) Scientific Reports 12 1 |
institution |
Open Polar |
collection |
OSKAR Bordeaux (Open Science Knowledge ARchive) |
op_collection_id |
ftoskarbordeaux |
language |
English |
topic |
Ecology Imaging Marine biology Microscopy Optical spectroscopy Sciences de l'environnement |
spellingShingle |
Ecology Imaging Marine biology Microscopy Optical spectroscopy Sciences de l'environnement GAILLARD, Melanie PARLANTI, Edith SOURZAC, Mahaut COUILLAUD, Franck GENEVOIS, Coralie BOUTRY, Sebastien RIGAUD, Christian DAVERAT, Francoise New insights into detecting alizarin from autofluorescence in marked glass eels |
topic_facet |
Ecology Imaging Marine biology Microscopy Optical spectroscopy Sciences de l'environnement |
description |
Alizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologies on the living body and fins of European glass eels (Anguilla anguilla L.). The findings demonstrated that the control group had a high autofluorescence at alizarin and eelGFP maxima bands. With fluorescence reflectance imaging (FRI), the eel living body autofluorescence impeded the detection of the marked eels. In contrast with experimental excitation-emission-matrix (EEM) fluorescence analyses, 99% of the marked eels were correctly assigned to their group from fluorescence analyses of their fin cellular contents. With epifluorometry (EPI), 100% of the marked eels were detected with the caudal fin tips when excited at 450–490 nm wavelengths due to a weaker autofluorescence signal. EEM and FRI assays unveiled an average fluorescence quenching 60% and 44% of the marked group respectively, in the alizarin and eelGFP maxima bands. The fluorescence quenching observed is discussed. Results will benefit experimental design by examining autofluorescence effects on mark detection and the development of non-invasive detection methods in this critically endangered species. |
format |
Other/Unknown Material |
author |
GAILLARD, Melanie PARLANTI, Edith SOURZAC, Mahaut COUILLAUD, Franck GENEVOIS, Coralie BOUTRY, Sebastien RIGAUD, Christian DAVERAT, Francoise |
author_facet |
GAILLARD, Melanie PARLANTI, Edith SOURZAC, Mahaut COUILLAUD, Franck GENEVOIS, Coralie BOUTRY, Sebastien RIGAUD, Christian DAVERAT, Francoise |
author_sort |
GAILLARD, Melanie |
title |
New insights into detecting alizarin from autofluorescence in marked glass eels |
title_short |
New insights into detecting alizarin from autofluorescence in marked glass eels |
title_full |
New insights into detecting alizarin from autofluorescence in marked glass eels |
title_fullStr |
New insights into detecting alizarin from autofluorescence in marked glass eels |
title_full_unstemmed |
New insights into detecting alizarin from autofluorescence in marked glass eels |
title_sort |
new insights into detecting alizarin from autofluorescence in marked glass eels |
publishDate |
2022 |
url |
https://oskar-bordeaux.fr/handle/20.500.12278/170392 https://hdl.handle.net/20.500.12278/170392 https://doi.org/10.1038/s41598-022-18440-0 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
2045-2322 https://oskar-bordeaux.fr/handle/20.500.12278/170392 doi:10.1038/s41598-022-18440-0 |
op_rights |
Attribution 3.0 United States open http://creativecommons.org/licenses/by/3.0/us/ CC BY |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/20.500.12278/170392 https://doi.org/10.1038/s41598-022-18440-0 |
container_title |
Scientific Reports |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766400427902894080 |