Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species
A systematic search for viral infection was performed in the isolated Kerguelen Islands, using a range of polyvalent genus-specific PCR assays. Barley yellow dwarf virus (BYDV) was detected in both introduced and native grasses such as Poa cookii. The geographical distribution of BYDV and its preval...
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/169344 2023-05-15T13:39:15+02:00 Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species SVANELLA-DUMAS, Laurence CANDRESSE, Thierry HULLÉ, Maurice MARAIS-COLOMBEL, Armelle 2013 https://oskar-bordeaux.fr/handle/20.500.12278/169344 https://hdl.handle.net/20.500.12278/169344 https://doi.org/10.1371/journal.pone.0067231 en eng Public Library of Science 1932-6203 https://oskar-bordeaux.fr/handle/20.500.12278/169344 doi:10.1371/journal.pone.0067231 Sciences du Vivant [q-bio]/Autre [q-bio.OT] Article de revue 2013 ftoskarbordeaux https://doi.org/20.500.12278/169344 https://doi.org/10.1371/journal.pone.0067231 2022-10-18T22:34:38Z A systematic search for viral infection was performed in the isolated Kerguelen Islands, using a range of polyvalent genus-specific PCR assays. Barley yellow dwarf virus (BYDV) was detected in both introduced and native grasses such as Poa cookii. The geographical distribution of BYDV and its prevalence in P. cookii were analyzed using samples collected from various sites of the archipelago. We estimate the average prevalence of BYDV to be 24.9% in P. cookii, with significant variability between sites. BYDV genetic diversity was assessed using sequence information from two genomic regions: the P3 open reading frame (ORF) (encoding the coat protein) and the hypervariable P6 ORF region. The phylogenetic analysis in the P3 region showed that BYDV sequences segregate into three major lineages, the most frequent of which (Ker-I cluster) showed close homology with BYDV-PAV-I isolates and had very low intra-lineage diversity (0.6%). A similarly low diversity was also recorded in the hypervariable P6 region, suggesting that Ker-I isolates derive from the recent introduction of BYDV-PAV-I. Divergence time estimation suggests that BYDV-PAV-I was likely introduced in the Kerguelen environment at the same time frame as its aphid vector, Rhopalosiphum padi, whose distribution shows good overlap with that of BYDV-Ker-I. The two other lineages show more than 22% amino acid divergence in the P3 region with other known species in the BYDV species complex, indicating that they represent distinct BYDV species. Using species-specific amplification primers, the distribution of these novel species was analyzed. The high prevalence of BYDV on native Poaceae and the presence of the vector R. padi, raises the question of its impact on the vulnerable plant communities of this remote ecosystem. Other/Unknown Material Antarc* Antarctic Kerguelen Islands OSKAR Bordeaux (Open Science Knowledge ARchive) Antarctic Kerguelen Kerguelen Islands |
institution |
Open Polar |
collection |
OSKAR Bordeaux (Open Science Knowledge ARchive) |
op_collection_id |
ftoskarbordeaux |
language |
English |
topic |
Sciences du Vivant [q-bio]/Autre [q-bio.OT] |
spellingShingle |
Sciences du Vivant [q-bio]/Autre [q-bio.OT] SVANELLA-DUMAS, Laurence CANDRESSE, Thierry HULLÉ, Maurice MARAIS-COLOMBEL, Armelle Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
topic_facet |
Sciences du Vivant [q-bio]/Autre [q-bio.OT] |
description |
A systematic search for viral infection was performed in the isolated Kerguelen Islands, using a range of polyvalent genus-specific PCR assays. Barley yellow dwarf virus (BYDV) was detected in both introduced and native grasses such as Poa cookii. The geographical distribution of BYDV and its prevalence in P. cookii were analyzed using samples collected from various sites of the archipelago. We estimate the average prevalence of BYDV to be 24.9% in P. cookii, with significant variability between sites. BYDV genetic diversity was assessed using sequence information from two genomic regions: the P3 open reading frame (ORF) (encoding the coat protein) and the hypervariable P6 ORF region. The phylogenetic analysis in the P3 region showed that BYDV sequences segregate into three major lineages, the most frequent of which (Ker-I cluster) showed close homology with BYDV-PAV-I isolates and had very low intra-lineage diversity (0.6%). A similarly low diversity was also recorded in the hypervariable P6 region, suggesting that Ker-I isolates derive from the recent introduction of BYDV-PAV-I. Divergence time estimation suggests that BYDV-PAV-I was likely introduced in the Kerguelen environment at the same time frame as its aphid vector, Rhopalosiphum padi, whose distribution shows good overlap with that of BYDV-Ker-I. The two other lineages show more than 22% amino acid divergence in the P3 region with other known species in the BYDV species complex, indicating that they represent distinct BYDV species. Using species-specific amplification primers, the distribution of these novel species was analyzed. The high prevalence of BYDV on native Poaceae and the presence of the vector R. padi, raises the question of its impact on the vulnerable plant communities of this remote ecosystem. |
format |
Other/Unknown Material |
author |
SVANELLA-DUMAS, Laurence CANDRESSE, Thierry HULLÉ, Maurice MARAIS-COLOMBEL, Armelle |
author_facet |
SVANELLA-DUMAS, Laurence CANDRESSE, Thierry HULLÉ, Maurice MARAIS-COLOMBEL, Armelle |
author_sort |
SVANELLA-DUMAS, Laurence |
title |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_short |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_full |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_fullStr |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_full_unstemmed |
Distribution of Barley yellow dwarf virus-PAV in the Sub-Antarctic Kerguelen Islands and characterization of two new [i]Luteovirus[/i] species |
title_sort |
distribution of barley yellow dwarf virus-pav in the sub-antarctic kerguelen islands and characterization of two new [i]luteovirus[/i] species |
publisher |
Public Library of Science |
publishDate |
2013 |
url |
https://oskar-bordeaux.fr/handle/20.500.12278/169344 https://hdl.handle.net/20.500.12278/169344 https://doi.org/10.1371/journal.pone.0067231 |
geographic |
Antarctic Kerguelen Kerguelen Islands |
geographic_facet |
Antarctic Kerguelen Kerguelen Islands |
genre |
Antarc* Antarctic Kerguelen Islands |
genre_facet |
Antarc* Antarctic Kerguelen Islands |
op_relation |
1932-6203 https://oskar-bordeaux.fr/handle/20.500.12278/169344 doi:10.1371/journal.pone.0067231 |
op_doi |
https://doi.org/20.500.12278/169344 https://doi.org/10.1371/journal.pone.0067231 |
_version_ |
1766116444299329536 |