Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles
A nanometric revolution is underway, promising technical innovations in a wide range of applications and leading to a potential boost in environmental discharges. The propensity of nanoparticles (NPs) to be transferred throughout trophic chains and to generate toxicity was mainly assessed in primary...
Published in: | Environmental Toxicology and Chemistry |
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Online Access: | https://doi.org/10.1002/etc.4858 |
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ftoskarbordeaux:oai:oskar-bordeaux.fr:20.500.12278/152006 2024-04-28T07:55:02+00:00 Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles PERRIER, Fanny BERTUCCI, Anthony PIERRON, Fabien FEURTET‐MAZEL, Agnès SIMON, Olivier KLOPP, Christophe CANDAUDAP, Frédéric POKROVSKI, Oleg ETCHEVERRIA, Bruno MORNET, Stéphane BAUDRIMONT, Magalie 2020 https://doi.org/10.1002/etc.4858 en eng Wiley 0730-7268 doi:10.1002/etc.4858 Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity Sciences du Vivant [q-bio]/Toxicologie/Ecotoxicologie Article de revue Article de synthèse 2020 ftoskarbordeaux https://doi.org/10.1002/etc.4858 2024-04-08T14:50:32Z A nanometric revolution is underway, promising technical innovations in a wide range of applications and leading to a potential boost in environmental discharges. The propensity of nanoparticles (NPs) to be transferred throughout trophic chains and to generate toxicity was mainly assessed in primary consumers, whereas a lack of knowledge for higher trophic levels persists. The present study focused on a predatory fish, the European eel (Anguilla anguilla) exposed to gold NPs (AuNPs; 10 nm, polyethylene glycol–coated) for 21 d at 3 concentration levels in food: 0 (NP0), 1 (NP1), and 10 (NP10) mg Au kg−1. Transfer was assessed by Au quantification in eel tissues, and transcriptomic responses in the liver and brain were revealed by a high‐throughput RNA‐sequencing approach. Eels fed at NP10 presented an erratic feeding behavior, whereas Au quantification only indicated transfer to intestine and kidney of NP1‐exposed eels. Sequencing of RNA was performed in NP0 and NP1 eels. A total of 258 genes and 156 genes were significantly differentially transcribed in response to AuNP trophic exposure in the liver and brain, respectively. Enrichment analysis highlighted modifications in the immune system–related processes in the liver. In addition, results pointed out a shared response of both organs regarding 13 genes, most of them being involved in immune functions. This finding may shed light on the mode of action and toxicity of AuNPs in fish. Approches à différentes échelles pour caractériser les interactions cellulaires, le transfert trophique et les impacts toxiques de nanoparticules métalliques chez les organismes aquatiques COntinental To coastal Ecosystems: evolution, adaptability and governance Article in Journal/Newspaper Anguilla anguilla OSKAR Bordeaux (Open Science Knowledge ARchive) Environmental Toxicology and Chemistry 39 12 2450 2461 |
institution |
Open Polar |
collection |
OSKAR Bordeaux (Open Science Knowledge ARchive) |
op_collection_id |
ftoskarbordeaux |
language |
English |
topic |
Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity Sciences du Vivant [q-bio]/Toxicologie/Ecotoxicologie |
spellingShingle |
Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity Sciences du Vivant [q-bio]/Toxicologie/Ecotoxicologie PERRIER, Fanny BERTUCCI, Anthony PIERRON, Fabien FEURTET‐MAZEL, Agnès SIMON, Olivier KLOPP, Christophe CANDAUDAP, Frédéric POKROVSKI, Oleg ETCHEVERRIA, Bruno MORNET, Stéphane BAUDRIMONT, Magalie Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
topic_facet |
Gold nanoparticles Anguilla anguilla RNAseq diet-borne exposure immunity Sciences du Vivant [q-bio]/Toxicologie/Ecotoxicologie |
description |
A nanometric revolution is underway, promising technical innovations in a wide range of applications and leading to a potential boost in environmental discharges. The propensity of nanoparticles (NPs) to be transferred throughout trophic chains and to generate toxicity was mainly assessed in primary consumers, whereas a lack of knowledge for higher trophic levels persists. The present study focused on a predatory fish, the European eel (Anguilla anguilla) exposed to gold NPs (AuNPs; 10 nm, polyethylene glycol–coated) for 21 d at 3 concentration levels in food: 0 (NP0), 1 (NP1), and 10 (NP10) mg Au kg−1. Transfer was assessed by Au quantification in eel tissues, and transcriptomic responses in the liver and brain were revealed by a high‐throughput RNA‐sequencing approach. Eels fed at NP10 presented an erratic feeding behavior, whereas Au quantification only indicated transfer to intestine and kidney of NP1‐exposed eels. Sequencing of RNA was performed in NP0 and NP1 eels. A total of 258 genes and 156 genes were significantly differentially transcribed in response to AuNP trophic exposure in the liver and brain, respectively. Enrichment analysis highlighted modifications in the immune system–related processes in the liver. In addition, results pointed out a shared response of both organs regarding 13 genes, most of them being involved in immune functions. This finding may shed light on the mode of action and toxicity of AuNPs in fish. Approches à différentes échelles pour caractériser les interactions cellulaires, le transfert trophique et les impacts toxiques de nanoparticules métalliques chez les organismes aquatiques COntinental To coastal Ecosystems: evolution, adaptability and governance |
format |
Article in Journal/Newspaper |
author |
PERRIER, Fanny BERTUCCI, Anthony PIERRON, Fabien FEURTET‐MAZEL, Agnès SIMON, Olivier KLOPP, Christophe CANDAUDAP, Frédéric POKROVSKI, Oleg ETCHEVERRIA, Bruno MORNET, Stéphane BAUDRIMONT, Magalie |
author_facet |
PERRIER, Fanny BERTUCCI, Anthony PIERRON, Fabien FEURTET‐MAZEL, Agnès SIMON, Olivier KLOPP, Christophe CANDAUDAP, Frédéric POKROVSKI, Oleg ETCHEVERRIA, Bruno MORNET, Stéphane BAUDRIMONT, Magalie |
author_sort |
PERRIER, Fanny |
title |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_short |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_full |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_fullStr |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_full_unstemmed |
Transfer and Transcriptomic Profiling in Liver and Brain of European Eels ( Anguilla anguilla ) After Diet‐borne Exposure to Gold Nanoparticles |
title_sort |
transfer and transcriptomic profiling in liver and brain of european eels ( anguilla anguilla ) after diet‐borne exposure to gold nanoparticles |
publisher |
Wiley |
publishDate |
2020 |
url |
https://doi.org/10.1002/etc.4858 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
0730-7268 doi:10.1002/etc.4858 |
op_doi |
https://doi.org/10.1002/etc.4858 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
39 |
container_issue |
12 |
container_start_page |
2450 |
op_container_end_page |
2461 |
_version_ |
1797578182771081216 |