Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms
Background: Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 h post fertilization, and it has been de...
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ftoregonstate:ir.library.oregonstate.edu:vh53x1868 2023-07-02T03:32:03+02:00 Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms Ventura, Alexander Durland, Evan De Wit, Pierre Langdon, Chris J. Fisheries and Wildlife Oregon State University. Sea Grant College Program https://ir.library.oregonstate.edu/concern/articles/vh53x1868 English [eng] eng BMC Oregon Sea Grant website: http://seagrant.oregonstate.edu/ https://ir.library.oregonstate.edu/concern/articles/vh53x1868 Attribution 4.0 (CC BY 4.0) Aragonite Gene expression Pacific oyster Ocean acidification Calcification Article ftoregonstate 2023-06-11T16:38:15Z Background: Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 h post fertilization, and it has been demonstrated that changes in water chemistry can cause delays in shell formation, shell deformations and higher mortality rates. In this study, we use the delay in shell formation associated with exposure to CO2-acidified seawater to identify genes correlated with initial shell deposition. Results: By fitting linear models to gene expression data in ambient and low aragonite saturation treatments, we are able to isolate 37 annotated genes correlated with initial larval shell formation, which can be categorized into 1) ion transporters, 2) shell matrix proteins and 3) protease inhibitors. Clustering of the gene expression data into co-expression networks further supports the result of the linear models, and also implies an important role of dynein motor proteins as transporters of cellular components during the initial shell formation process. Conclusions: Using an RNA-Seq approach with high temporal resolution allows us to identify a conceptual model for how oyster larval calcification is initiated. This work provides a foundation for further studies on how genetic variation in these identified genes could affect fitness of oyster populations subjected to future environmental changes, such as ocean acidification. Keywords: Crassostrea gigas, Gene expression, Larvae, Ocean acidification, Aragonite, Calcification Article in Journal/Newspaper Crassostrea gigas Ocean acidification Pacific oyster ScholarsArchive@OSU (Oregon State University) Pacific |
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ScholarsArchive@OSU (Oregon State University) |
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ftoregonstate |
language |
English |
topic |
Aragonite Gene expression Pacific oyster Ocean acidification Calcification |
spellingShingle |
Aragonite Gene expression Pacific oyster Ocean acidification Calcification Ventura, Alexander Durland, Evan De Wit, Pierre Langdon, Chris J. Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
topic_facet |
Aragonite Gene expression Pacific oyster Ocean acidification Calcification |
description |
Background: Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 h post fertilization, and it has been demonstrated that changes in water chemistry can cause delays in shell formation, shell deformations and higher mortality rates. In this study, we use the delay in shell formation associated with exposure to CO2-acidified seawater to identify genes correlated with initial shell deposition. Results: By fitting linear models to gene expression data in ambient and low aragonite saturation treatments, we are able to isolate 37 annotated genes correlated with initial larval shell formation, which can be categorized into 1) ion transporters, 2) shell matrix proteins and 3) protease inhibitors. Clustering of the gene expression data into co-expression networks further supports the result of the linear models, and also implies an important role of dynein motor proteins as transporters of cellular components during the initial shell formation process. Conclusions: Using an RNA-Seq approach with high temporal resolution allows us to identify a conceptual model for how oyster larval calcification is initiated. This work provides a foundation for further studies on how genetic variation in these identified genes could affect fitness of oyster populations subjected to future environmental changes, such as ocean acidification. Keywords: Crassostrea gigas, Gene expression, Larvae, Ocean acidification, Aragonite, Calcification |
author2 |
Fisheries and Wildlife Oregon State University. Sea Grant College Program |
format |
Article in Journal/Newspaper |
author |
Ventura, Alexander Durland, Evan De Wit, Pierre Langdon, Chris J. |
author_facet |
Ventura, Alexander Durland, Evan De Wit, Pierre Langdon, Chris J. |
author_sort |
Ventura, Alexander |
title |
Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_short |
Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_full |
Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_fullStr |
Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_full_unstemmed |
Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
title_sort |
gene expression correlated with delay in shell formation in larval pacific oysters (crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms |
publisher |
BMC |
url |
https://ir.library.oregonstate.edu/concern/articles/vh53x1868 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_relation |
Oregon Sea Grant website: http://seagrant.oregonstate.edu/ https://ir.library.oregonstate.edu/concern/articles/vh53x1868 |
op_rights |
Attribution 4.0 (CC BY 4.0) |
_version_ |
1770271514001670144 |