Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera)
The primary goal of this research was to determine the concentration and composition of nucleotides, as nucleoside monophosphates and nucleobases, in the underutilized reproductive organs (milt and roe) of Alaska pollock (Gadus chalcogrammus) and yellowfin sole (Limanda aspera). Changes in concentra...
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ftoregonstate:ir.library.oregonstate.edu:jd4733902 2023-06-11T04:03:08+02:00 Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera) Nelson, Kevin A. DeWitt, Christina M. Park, Jae W. Penner, Michael H. Chaplen, Frank W. R. Food Science and Technology 91 pages https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/jd4733902 English [eng] eng Oregon State University Seafood Research and Education Center Website: https://osuseafoodlab.oregonstate.edu/ The Pollock Conservation Cooperative Research Center: https://www.pccrc.org/ https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/jd4733902 All rights reserved Masters Thesis ftoregonstate 2023-05-07T17:53:48Z The primary goal of this research was to determine the concentration and composition of nucleotides, as nucleoside monophosphates and nucleobases, in the underutilized reproductive organs (milt and roe) of Alaska pollock (Gadus chalcogrammus) and yellowfin sole (Limanda aspera). Changes in concentrations of these nucleotides and nucleotide analogs were observed with respect to various storage and processing techniques. Samples were freeze-dried (-40°C), dessicated (70°C) and oven dried in laboratory ovens at two temperatures (100°C and 120°C). Several extraction/digestion techniques were employed to evaluate total nucleotides present. DNA extracts were analyzed for total nucleotide monophosphates and nucleotide composition of the DNA extract. Methods for extracting total DNA from reproductive tissues from pollock were evaluated using various chemical techniques including acid digestion, phenol-chloroform extraction and QIAGEN® extractions kits. Once extracted, DNA was digested into nucleotide monomers (deoxyadenosine monophosphate (dAMP), deoxyguanosine monophosphate (dGMP), deoxycytidine monophosphate (dCMP) and thymidine monophosphate (TMP)) using an enzymatic (endonuclease) digestion. In a different analysis, total nucleobases and nucleobase composition as purine and pyrimidine bases (cytosine, guanine, thymine, adenine, uracil, hypoxanthine and xanthine) were also determined; these results were used to assess total DNA, RNA and free nucleotides in dried milt meal. Quantification of the nucleotides was achieved using a reverse-phase high performance liquid chromatography (RP-HPLC) system. Degradation by-products of adenosine monophosphate (as adenosine monophosphate (AMP), inosine monophosphate (IMP), inosine and hypoxanthine) were also determined via HPLC after perchloric acid digestion. This knowledge will serve as a basis of determining the feasibility of creating a consistent, cost-effective source of nucleotide or nucleotide analogs for potential use in the formulation of aquaculture feed, nutraceuticals ... Master Thesis alaska pollock Alaska ScholarsArchive@OSU (Oregon State University) |
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Open Polar |
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ScholarsArchive@OSU (Oregon State University) |
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ftoregonstate |
language |
English |
description |
The primary goal of this research was to determine the concentration and composition of nucleotides, as nucleoside monophosphates and nucleobases, in the underutilized reproductive organs (milt and roe) of Alaska pollock (Gadus chalcogrammus) and yellowfin sole (Limanda aspera). Changes in concentrations of these nucleotides and nucleotide analogs were observed with respect to various storage and processing techniques. Samples were freeze-dried (-40°C), dessicated (70°C) and oven dried in laboratory ovens at two temperatures (100°C and 120°C). Several extraction/digestion techniques were employed to evaluate total nucleotides present. DNA extracts were analyzed for total nucleotide monophosphates and nucleotide composition of the DNA extract. Methods for extracting total DNA from reproductive tissues from pollock were evaluated using various chemical techniques including acid digestion, phenol-chloroform extraction and QIAGEN® extractions kits. Once extracted, DNA was digested into nucleotide monomers (deoxyadenosine monophosphate (dAMP), deoxyguanosine monophosphate (dGMP), deoxycytidine monophosphate (dCMP) and thymidine monophosphate (TMP)) using an enzymatic (endonuclease) digestion. In a different analysis, total nucleobases and nucleobase composition as purine and pyrimidine bases (cytosine, guanine, thymine, adenine, uracil, hypoxanthine and xanthine) were also determined; these results were used to assess total DNA, RNA and free nucleotides in dried milt meal. Quantification of the nucleotides was achieved using a reverse-phase high performance liquid chromatography (RP-HPLC) system. Degradation by-products of adenosine monophosphate (as adenosine monophosphate (AMP), inosine monophosphate (IMP), inosine and hypoxanthine) were also determined via HPLC after perchloric acid digestion. This knowledge will serve as a basis of determining the feasibility of creating a consistent, cost-effective source of nucleotide or nucleotide analogs for potential use in the formulation of aquaculture feed, nutraceuticals ... |
author2 |
DeWitt, Christina M. Park, Jae W. Penner, Michael H. Chaplen, Frank W. R. Food Science and Technology |
format |
Master Thesis |
author |
Nelson, Kevin A. |
spellingShingle |
Nelson, Kevin A. Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera) |
author_facet |
Nelson, Kevin A. |
author_sort |
Nelson, Kevin A. |
title |
Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera) |
title_short |
Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera) |
title_full |
Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera) |
title_fullStr |
Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera) |
title_full_unstemmed |
Determination of Total Nucleotides, Nitrogenous Bases and Degradation By-Products with Respect to Various Processing and Preservation Techniques for Alaska Pollock (Gadus chalcogrammus) and Yellowfin Sole (Limanada aspera) |
title_sort |
determination of total nucleotides, nitrogenous bases and degradation by-products with respect to various processing and preservation techniques for alaska pollock (gadus chalcogrammus) and yellowfin sole (limanada aspera) |
publisher |
Oregon State University |
url |
https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/jd4733902 |
genre |
alaska pollock Alaska |
genre_facet |
alaska pollock Alaska |
op_relation |
Seafood Research and Education Center Website: https://osuseafoodlab.oregonstate.edu/ The Pollock Conservation Cooperative Research Center: https://www.pccrc.org/ https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/jd4733902 |
op_rights |
All rights reserved |
_version_ |
1768377237003829248 |